Nutritional targeting modification of silkworm pupae oil catalyzed by a smart hydrogel immobilized lipase

2021 ◽  
Author(s):  
Jin-Zheng Wang ◽  
Cheng-Kun Wu ◽  
Cheng-Hai Yan ◽  
Huan Chen ◽  
Shuai You ◽  
...  
Keyword(s):  
Immobilized Lipase ◽  
Nutritional Supplement ◽  
Relative Content ◽  
Smart Hydrogel ◽  
Microfluidic Reactor ◽  
Silkworm Pupae ◽  
Silkworm Pupa

To prepare a nutritional supplement using silkworm pupa oil (SPO) as a feedstock, a microfluidic reactor with smart hydrogel immobilized lipase was firstly constructed to reduce the relative content of...

scholarly journals Correction: Nutritional targeting modification of silkworm pupae oil catalyzed by a smart hydrogel immobilized lipase

2021 ◽  
Author(s):  
Jin-Zheng Wang ◽  
Cheng-Kun Wu ◽  
Cheng-Hai Yan ◽  
Huan Chen ◽  
Shuai You ◽  
...  
Keyword(s):  
Immobilized Lipase ◽  
Smart Hydrogel ◽  
Silkworm Pupae

Correction for ‘Nutritional targeting modification of silkworm pupae oil catalyzed by a smart hydrogel immobilized lipase’ by Jin-Zheng Wang et al., Food Funct., 2021, 12, 6240–6253, DOI: 10.1039/D1FO00913C.

An Immobilized Lipase Microfluidic Reactor for Enantioselective Hydrolysis of Ester

2009 ◽  
Vol 38 (3) ◽  
pp. 262-263 ◽  
Author(s):  
Yan Gao ◽  
Runtao Zhong ◽  
Jianhua Qin ◽  
Bingcheng Lin
Keyword(s):  
Immobilized Lipase ◽  
Enantioselective Hydrolysis ◽  
Microfluidic Reactor ◽  
Hydrolysis Of

scholarly journals Perbedaan Pertumbuhan Artemia salina Pada Perlakuan Variasi Dosis Pakan Jus Pupa Ulat Sutra (Samia cynthia)

2020 ◽  
Vol 6 (1) ◽  
pp. 10-20
Author(s):  
Agus Dharmawan ◽  
Nurul Hikmah ◽  
Mita Larasati
Keyword(s):  
Culture Media ◽  
Average Length ◽  
Abiotic Factors ◽  
Artemia Salina ◽  
Dose Treatment ◽  
Silkworm Pupae ◽  
Silk Worm ◽  
Silkworm Pupa ◽  
Protein Feed ◽  
Observation Results

High protein feed are very necessary in the cultivation industry of Artemia salina. One of the proteins sources that can be used is silk worm pupae (Samia cynthia). The aim of this study was to determine the differences in growth of Artemia salina with the treatment of silkworm pupa juice feeding at various doses. This study uses 4 levels of treatment, namely 0.16 ml; 0.33 ml; 0.50 ml and 0.67 ml per liter with 6 replications, which are given daily for 21 days. Abiotic factors of culture media (salinity, temperature, pH, and DO) are controlled and in accordance with the needs of Artemia salina's life. Observation results on the 15th day, 17th day, and 19th day showed that there were differences in the number of Artemia salina due to the treatment of variations in the feed dose of silkworm pupae juice (Samia cynthia) with 0.67 ml feed dosage treatment having the highest average number and significantly different from other dosage treatments. Observations on the 13th day, 15th day and 21st day showed differences in the growth length of Artemia salina due to the variation of the dosage treatment of silkworm pupa juice (Samia cynthia) with 0.50 ml dose treatment having the highest average length and significantly different from other treatments. Keywords: Artemia salina, growth, feed, silkworm pupae ABSTRAK Pakan yang mengandung protein tinggi sangat diperlukan dalam budidaya Artemia salina. Salah satu sumber protein yang dapat digunakan adalah pupa ulat sutra (Samia cynthia). Tujuan penelitian ini untuk mengetahui perbedaan pertumbuhan Artemia salina dengan perlakuan pemberian pakan jus pupa ulat sutra pada berbagai dosis. Penelitian ini menggunakan 4 taraf perlakuan yaitu dosis 0,16 ml; 0,33 ml; 0,50 ml dan 0,67 ml per liter dengan 6 ulangan, yang diberikan setiap hari selama 21 hari. Faktor abiotik media kultur (salinitas, suhu, pH, dan DO) terkontrol dan sesuai dengan kebutuhan hidup Artemia salina. Hasil pengamatan hari ke-15, hari ke-17, dan hari ke-19 menunjukkan adanya perbedaan jumlah Artemia salina akibat pemberian perlakuan variasi dosis pakan jus pupa ulat sutra (Samia chyntia) dengan perlakuan dosisi pakan 0,67 ml memiliki retata jumlah tertinggi dan berbeda nyata dengan perlakuan dosis lainnya. Hasil pengamatan pada hari ke-13, hari ke-15 dan hari ke-21 menunjukkan adanya perbedaan pertumbuhan panjang Artemia salina akibat pemberian perlakuan variasi dosis pakan jus pupa ulat sutra (Samia chyntia) dengan perlakuan dosis 0,50 ml memiliki rerata panjang tertinggi dan berbeda nyata dengan perlakuan yang lain. Kata kunci: Artemia salina, pertumbuhan, pakan, pupa ulat sutra

scholarly journals Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Dean Huang ◽  
Yu-Chan Chao ◽  
Zhengbing Lv ◽  
Jia-Tsrong Jan ◽  
Yu-Chih Yang ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Immune Responses ◽  
Avian Influenza Virus ◽  
Matrix Protein ◽  
Haemagglutination Inhibition ◽  
Virus Like Particles ◽  
Silkworm Pupae ◽  
Ifn Γ ◽  
Silkworm Pupa

Abstract Introduction Novel clade 2.3.4.4 H5 highly pathogenic avian influenza virus (HPAIV) outbreaks have occurred since early 2015 in Taiwan and impacted the island economically, like they have many countries. This research investigates the immunogenicity of two HPAIV-like particles to assess their promise as vaccine candidates. Material and Methods The haemagglutinin (HA) gene derived from clade 2.3.4.4 H5 HPAIV and matrix protein 1 (M1) gene were cloned into the pFastBac Dual baculovirus vector. The resulting recombinant viruses were expressed in Spodoptera frugiperda moth (Sf)21 cells and silkworm pupae to generate Sf21 virus-like particles (VLP) and silkworm pupa VLP. Two-week-old specific pathogen–free chickens were immunised and their humoral and cellular immune responses were analysed. Results The silkworm pupa VLP had higher haemagglutination competence. Both VLP types elicited haemagglutination inhibition antibodies, anti-HA antibodies, splenic interferon gamma (IFN-γ) and interleukin 4 (IL-4) mRNA expression, and CD4+/CD8+ ratio elevation. However, chickens receiving silkworm pupa VLP exhibited a significantly higher anti-HA antibody titre in ELISA after vaccination. Although Sf21 VLP recipients expressed more IFN-γ and IL-4, the increase in IFN-γ did not significantly raise the CD4+/CD8+ ratio and the increase in IL-4 did not promote anti-HA antibodies. Conclusion Both VLP systems possess desirable immunogenicity in vivo. However, in respect of immunogenic efficacy and the production cost, pupa VLP may be the superior vaccine candidate against clade 2.3.4.4 H5 HPAIV infection.

scholarly journals Digestibility of dry matter and nutrients from three ingredients by the carps, Labeo fimbriatus (Bloch, 1795) and Cyprinus carpio Linnaeus, 1758 with a note on digestive enzyme activity

2017 ◽  
Vol 64 (3) ◽  
Author(s):  
B. Gangadhar ◽  
H. Umalatha ◽  
H. Ganesh ◽  
S. Saurabh ◽  
N. Sridhar
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Dry Matter ◽  
Digestive Enzyme ◽  
Control Diet ◽  
Protein Digestibility ◽  
Groundnut Oil ◽  
Crude Fibre ◽  
Silkworm Pupae ◽  
Silkworm Pupa

This study was conducted in indoor, aerated plastic tubs to estimate the digestibility of dry matter and nutrients by the carps, Labeo fimbriatus (Bloch, 1795) and Cyprinus carpio Linnaeus, 1758, from three feed ingredients viz., azolla, soybean meal and silkworm pupa incorporated at levels ranging from 0-40% in a diet comprising equal amounts of rice bran and groundnut oil cake using crude fibre as the marker. Activity of the major digestive enzymes was also estimated in the gut of fish at the end of the experimental trial. Utilisation of azolla protein was comparable with that of control diet up to 20% incorporation in both L. fimbriatus and common carp. Major nutrients from soybean were better utilised up to 40% incorporation by the two species. Protein digestibility from silkworm pupae increased at 20% incorporation and decreased at 40% in L. fimbriatus, while fat and nitrogen-free extract digestibility was higher at 20-40% incorporation. In common carp, higher digestibility of protein and fat was recorded at 10-30% inclusion levels of silkworm pupa.

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