Tailored rigidity of W/O Pickering emulsions using diacylglycerol-based surface-active solid lipid nanoparticles

2021 ◽  
Author(s):  
Guoyan Li ◽  
Wan Jun Lee ◽  
Chin Ping Tan ◽  
Oi-Ming Lai ◽  
Yong Wang ◽  
...  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Colloidal Stability ◽  
Lipid Nanoparticles ◽  
Pickering Emulsions ◽  
Solid Lipid ◽  
Surface Active ◽  
Long Chain

Pickering water-in-oil (W/O) emulsions were fabricated by using medium-long chain diacylglycerol (MLCD)-based solid lipid nanoparticles (SLNs) and the connection between the characteristics of the SLNs and the colloidal stability of...

scholarly journals Correction: Synthetic ultra-long chain fatty acyl based amphiphilic lipids as a dual function excipient for the production of surfactant-free solid lipid nanoparticles (SF-SLNs): a physico-chemical study

2016 ◽  
Vol 18 (15) ◽  
pp. 4312-4312
Author(s):  
Wei Wei ◽  
Xiaonan Lu ◽  
Zegao Wang ◽  
Mingdong Dong ◽  
Fengqin Feng ◽  
...  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Chemical Study ◽  
Lipid Nanoparticles ◽  
Fatty Acyl ◽  
Dual Function ◽  
Solid Lipid ◽  
Physico Chemical ◽  
Long Chain

Correction for ‘Synthetic ultra-long chain fatty acyl based amphiphilic lipids as a dual function excipient for the production of surfactant-free solid lipid nanoparticles (SF-SLNs): a physico-chemical study’ by Wei Wei et al., Green Chem., 2016, DOI: 10.1039/c6gc00866f.

scholarly journals Microemulsion-Based Solid Lipid Nanoparticles As Emulsion Stabilisers

2021 ◽  
Author(s):  
Elham H. Hazfi
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Continuous Phase ◽  
Tween 20 ◽  
Microemulsion System ◽  
Solid Lipid ◽  
Oil In Water ◽  
Phase Crystal ◽  
Surface Active ◽  
Oil Water

The preparation and properties of water-in-oil (W/O) emulsions stabilised solely by adsorbed surface-active solid lipid nanoparticles (SLNs) at the oil-water interface were studied. Monostearin-based SLNs were prepared using food-grade micoremulsions as nanoscle 'reactors'. Hot oil-in-water (O/W) microemulsions (70°C) consisting of monostearin, Tween 20, ethanol and water were crash-cooled to 4°C to promote the liquid-solid transition of the monostearin and thus develop sub-micron solid lipid particles. SLNs obtained from the cooled microemulsions were partially stabilised with addition to lecithin (0.5% w/w) to the microemulsion system. With 2% (w/w) added monstearin, the W/O emulsion was stable for the 14 days of study. The microstructure of the emulsions revealed the presence of two stabilisation mechanisms, namely Pickering-type and continuous phase crystal network stabilisation, which both contributed to slowing dispersed droplet coalescence. Overall, this study demonstrated that surface-active SLNs developed using a microemulsion technique could effectively kinetically stabilise model W/O emulsions.

scholarly journals Solid Lipid Nanoparticles for Dibucaine Sustained Release

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 231 ◽  
Author(s):  
Raquel de M. Barbosa ◽  
Ligia Ribeiro ◽  
Bruna Casadei ◽  
Camila da Silva ◽  
Viviane Queiróz ◽  
...  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Encapsulation Efficiency ◽  
Colloidal Stability ◽  
Lipid Nanoparticles ◽  
In Vitro Cytotoxicity ◽  
Tail Flick ◽  
Release Kinetic ◽  
Solid Lipid

Dibucaine (DBC) is among the more potent long-acting local anesthetics (LA), and it is also one of the most toxic. Over the last decades, solid lipid nanoparticles (SLN) have been developed as promising carriers for drug delivery. In this study, SLN formulations were prepared with the aim of prolonging DBC release and reducing its toxicity. To this end, SLN composed of two different lipid matrices and prepared by two different hot-emulsion techniques (high-pressure procedure and sonication) were compared. The colloidal stability of the SLN formulations was tracked in terms of particle size (nm), polydispersity index (PDI), and zeta potential (mV) for 240 days at 4 °C; the DBC encapsulation efficiency was determined by the ultrafiltration/centrifugation method. The formulations were characterized by differential scanning calorimetry (DSC), electron paramagnetic resonance (EPR), and release kinetic experiments. Finally, the in vitro cytotoxicity against 3T3 fibroblast and HaCaT cells was determined, and the in vivo analgesic action was assessed using the tail flick test in rats. Both of the homogenization procedures were found suitable to produce particles in the 200 nm range, with good shelf stability (240 days) and high DBC encapsulation efficiency (~72–89%). DSC results disclosed structural information on the nanoparticles, such as the lower crystallinity of the lipid core vs. the bulk lipid. EPR measurements provided evidence of DBC partitioning in both SLNs. In vitro (cytotoxicity) and in vivo (tail flick) experiments revealed that the encapsulation of DBC into nanoparticles reduces its intrinsic cytotoxicity and prolongs the anesthetic effect, respectively. These results show that the SLNs produced are safe and have great potential to extend the applications of dibucaine by enhancing its bioavailability.

Synthetic ultra-long chain fatty acyl based amphiphilic lipids as a dual function excipient for the production of surfactant-free solid lipid nanoparticles (SF-SLNs): a physico-chemical study

2016 ◽  
Vol 18 (14) ◽  
pp. 3962-3971 ◽  
Author(s):  
Wei Wei ◽  
Xiaonan Lu ◽  
Zegao Wang ◽  
Mingdong Dong ◽  
Fengqin Feng ◽  
...  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Chemical Study ◽  
Lipid Nanoparticles ◽  
Fatty Acyl ◽  
Dual Function ◽  
Solid Lipid ◽  
Physico Chemical ◽  
Long Chain

Behenoyl (22 : 0) based amphiphilic lipids are synthesized, some of which demonstrate excellent dual functionality as both solid excipients and emulsifiers in producing uniform solid lipid nanoparticles (SLNs).

scholarly journals Lyophilized Drug-Loaded Solid Lipid Nanoparticles Formulated with Beeswax and Theobroma Oil

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 908
Author(s):  
Hilda Amekyeh ◽  
Nashiru Billa
Keyword(s):  
Drug Release ◽  
Solid Lipid Nanoparticles ◽  
Colloidal Stability ◽  
Fold Increase ◽  
Active Pharmaceutical Ingredient ◽  
Lipid Nanoparticles ◽  
Systemic Availability ◽  
Solid Lipid ◽  
And Storage ◽  
Higuchi Model

Solid lipid nanoparticles (SLNs) have the potential to enhance the systemic availability of an active pharmaceutical ingredient (API) or reduce its toxicity through uptake of the SLNs from the gastrointestinal tract or controlled release of the API, respectively. In both aspects, the responses of the lipid matrix to external challenges is crucial. Here, we evaluate the effects of lyophilization on key responses of 1:1 beeswax–theobroma oil matrix SLNs using three model drugs: amphotericin B (AMB), paracetamol (PAR), and sulfasalazine (SSZ). Fresh SLNs were stable with sizes ranging between 206.5–236.9 nm. Lyophilization and storage for 24 months (4–8 °C) caused a 1.6- and 1.5-fold increase in size, respectively, in all three SLNs. Zeta potential was >60 mV in fresh, stored, and lyophilized SLNs, indicating good colloidal stability. Drug release was not significantly affected by lyophilization up to 8 h. Drug release percentages at end time were 11.8 ± 0.4, 65.9 ± 0.04, and 31.4 ± 1.95% from fresh AMB-SLNs, PAR-SLNs, and SSZ-SLNs, respectively, and 11.4 ± 0.4, 76.04 ± 0.21, and 31.6 ± 0.33% from lyophilized SLNs, respectively. Thus, rate of release is dependent on API solubility (AMB < SSZ < PAR). Drug release from each matrix followed the Higuchi model and was not affected by lyophilization. The above SLNs show potential for use in delivering hydrophilic and lipophilic drugs.

scholarly journals Co-Encapsulation of Mitoxantrone and β-Elemene in Solid Lipid Nanoparticles to Overcome Multidrug Resistance in Leukemia

Pharmaceutics ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 191 ◽  
Author(s):  
Kambere Amerigos Daddy J.C. ◽  
Minglei Chen ◽  
Faisal Raza ◽  
Yanyu Xiao ◽  
Zhigui Su ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Cellular Uptake ◽  
Solid Lipid Nanoparticles ◽  
Colloidal Stability ◽  
Fluorescence Probe ◽  
Synergistic Effects ◽  
K562 Cells ◽  
Lipid Nanoparticles ◽  
In Vivo Studies ◽  
Solid Lipid

Multidrug resistance (MDR) due to P-glycoprotein (P-gp) overexpression is a major obstacle to successful leukemia chemotherapy. The combination of anticancer chemotherapy with a chemosensitizer of P-gp inhibitor is promising to overcome MDR, generate synergistic effects, and maximize the treatment effect. Herein, we co-encapsulated a chemotherapeutic drug of mitoxantrone (MTO) and a P-gp inhibitor of β-elemene (βE) in solid lipid nanoparticles (MTO/βE-SLNs) for reversing MDR in leukemia. The MTO/βE-SLNs with about 120 nm particle size possessed good colloidal stability and sustained release behavior. For the cellular uptake study, doxorubicin (DOX) was used as a fluorescence probe to construct SLNs. The results revealed that MTO/βE-SLNs could be effectively internalized by both K562/DOX and K562 cells through the pathway of caveolate-mediated endocytosis. Under the optimized combination ratio of MTO and βE, the in vitro cytotoxicity study indicated that MTO/βE-SLNs showed a better antitumor efficacy in both K562/DOX and K562 cells than other MTO formulations. The enhanced cytotoxicity of MTO/βE-SLNs was due to the increased cellular uptake and blockage of intracellular ATP production and P-gp efflux by βE. More importantly, the in vivo studies revealed that MTO/βE-SLNs could significantly prolong the circulation time and increase plasma half-life of both MTO and βE, accumulate into tumor and exhibit a much higher anti-leukemia effect with MDR than other MTO formulations. These findings suggest MTO/βE-SLNs as a potential combined therapeutic strategy for overcoming MDR in leukemia.

scholarly journals Microemulsion-Based Solid Lipid Nanoparticles As Emulsion Stabilisers

2021 ◽  
Author(s):  
Elham H. Hazfi
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Continuous Phase ◽  
Tween 20 ◽  
Microemulsion System ◽  
Solid Lipid ◽  
Oil In Water ◽  
Phase Crystal ◽  
Surface Active ◽  
Oil Water

The preparation and properties of water-in-oil (W/O) emulsions stabilised solely by adsorbed surface-active solid lipid nanoparticles (SLNs) at the oil-water interface were studied. Monostearin-based SLNs were prepared using food-grade micoremulsions as nanoscle 'reactors'. Hot oil-in-water (O/W) microemulsions (70°C) consisting of monostearin, Tween 20, ethanol and water were crash-cooled to 4°C to promote the liquid-solid transition of the monostearin and thus develop sub-micron solid lipid particles. SLNs obtained from the cooled microemulsions were partially stabilised with addition to lecithin (0.5% w/w) to the microemulsion system. With 2% (w/w) added monstearin, the W/O emulsion was stable for the 14 days of study. The microstructure of the emulsions revealed the presence of two stabilisation mechanisms, namely Pickering-type and continuous phase crystal network stabilisation, which both contributed to slowing dispersed droplet coalescence. Overall, this study demonstrated that surface-active SLNs developed using a microemulsion technique could effectively kinetically stabilise model W/O emulsions.

scholarly journals Influence of Process Design on the Preparation of Solid Lipid Nanoparticles by an Ultrasonic-Nanoemulsification Method

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1265
Author(s):  
Agata Pucek-Kaczmarek
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Colloidal Stability ◽  
Lipid Nanoparticles ◽  
Biological Properties ◽  
Ionic Surfactant ◽  
Lipid Phase ◽  
Scanning Calorimetry ◽  
Present Contribution ◽  
Stabilizing Agents ◽  
Solid Lipid

In recent years, lipid-based nanosystems have emerged as a promising class of nanocarriers for encapsulating many active agents. Solid lipid nanoparticles (SLNs) provide good stability (colloidal as well as physical) and high biocompatibility. Appropriate design of the carrier structure through a selection of components and preparation methods allows us to obtain formulations with desired physicochemical parameters and biological properties. The present contribution has been carried out to investigate SLNs containing biocompatible phosphatidylcholine mixed with non-ionic surfactant Tween 60 as stabilizing agents. The internal lipid phase consisted of glyceryl monostearate was confirmed as safe for drug delivery by the Food and Drug Administration. The SLNs were fabricated by ultrasonic-nanoemulsification method. The preparation process was optimized in regard to variable parameters such as ultrasonication time and used amplitude and number of cycles. The sizes of the studied nanoparticles along with the size distribution were determined by dynamic light scattering (DLS), while shape and morphology were determined by atomic force microscopy (AFM) and transmission electron microscopy (TEM). The colloidal stability was measured by a turbidimetric method. The physical state of SLNs was characterized using differential scanning calorimetry (DSC). The obtained results indicate that the proposed SLNs may provide great potential for design and preparation of novel delivery nanosystems with a variety of possible applications.

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