In vitro fecal fermentation profiles and microbiota responses of pulse cell wall polysaccharides: Enterotype effect

2021 ◽  
Author(s):  
Miaomiao Yu ◽  
Seda Arıoğlu-Tuncil ◽  
Zhuqing Xie ◽  
Xiong Fu ◽  
Qiang Huang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Gut Microbiota ◽  
Cell Wall Polysaccharides

Gut microbiota community of individuals is predominated by diverse fiber-utilizing bacteria, and might have distinct fermentation outcomes for a given dietary substrate. In this research, we isolated pea cell wall...

scholarly journals Procyanidin—Cell Wall Interactions within Apple Matrices Decrease the Metabolization of Procyanidins by the Human Gut Microbiota and the Anti-Inflammatory Effect of the Resulting Microbial Metabolome In Vitro

Nutrients ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 664 ◽  
Author(s):  
Carine Le Bourvellec ◽  
Priscilla Bagano Vilas Boas ◽  
Pascale Lepercq ◽  
Sophie Comtet-Marre ◽  
Pauline Auffret ◽  
...  
Keyword(s):  
Cell Wall ◽  
Gut Microbiota ◽  
Biological Effects ◽  
Bioactive Metabolites ◽  
Cell Wall Polysaccharides ◽  
Covalent Bonds ◽  
The Matrix ◽  
Anti Inflammatory ◽  
The Impact

B-type oligomeric procyanidins in apples constitute an important source of polyphenols in the human diet. Their role in health is not known, although it is suggested that they generate beneficial bioactive compounds upon metabolization by the gut microbiota. During apple processing, procyanidins interact with cell-wall polysaccharides and form stable complexes. These interactions need to be taken into consideration in order to better assess the biological effects of fruit constituents. Our objectives were to evaluate the impact of these interactions on the microbial metabolization of cell walls and procyanidins, and to investigate the potential anti-inflammatory activity of the resulting metabolome, in addition to analyzing the taxonomical changes which the microbiota undergo. In vitro fermentation of three model apple matrices with microbiota from 4 healthy donors showed that the binding of procyanidins to cell-wall polysaccharides, whether covalently or non-covalently, substantially reduced procyanidin degradation. Although cell wall-unbound procyanidins negatively affected carbohydrate fermentation, they generated more hydroxyphenylvaleric acid than bound procyanidins, and increased the abundance of Adlercreutzia and Gordonibacter genera. The best results in terms of production of anti-inflammatory bioactive metabolites were observed from the apple matrix with no bonds between procyanidins and cell wall polysaccharides, although the matrix with non-covalent bonds was not far behind.

scholarly journals PUL-Mediated Plant Cell Wall Polysaccharide Utilization in the Gut Bacteroidetes

2021 ◽  
Vol 22 (6) ◽  
pp. 3077
Author(s):  
Zhenzhen Hao ◽  
Xiaolu Wang ◽  
Haomeng Yang ◽  
Tao Tu ◽  
Jie Zhang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Microbial Community ◽  
Gut Microbiota ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Polysaccharide ◽  
Prominent Feature ◽  
Cell Wall Polysaccharides ◽  
Gut Microbial Community

Plant cell wall polysaccharides (PCWP) are abundantly present in the food of humans and feed of livestock. Mammalians by themselves cannot degrade PCWP but rather depend on microbes resident in the gut intestine for deconstruction. The dominant Bacteroidetes in the gut microbial community are such bacteria with PCWP-degrading ability. The polysaccharide utilization systems (PUL) responsible for PCWP degradation and utilization are a prominent feature of Bacteroidetes. In recent years, there have been tremendous efforts in elucidating how PULs assist Bacteroidetes to assimilate carbon and acquire energy from PCWP. Here, we will review the PUL-mediated plant cell wall polysaccharides utilization in the gut Bacteroidetes focusing on cellulose, xylan, mannan, and pectin utilization and discuss how the mechanisms can be exploited to modulate the gut microbiota.

Effects of simulated digestion in vitro on cell wall polysaccharides from kiwifruit (Actinidia spp.)

2012 ◽  
Vol 133 (1) ◽  
pp. 132-139 ◽  
Author(s):  
Susan M. Carnachan ◽  
Tracey J. Bootten ◽  
Suman Mishra ◽  
John A. Monro ◽  
Ian M. Sims
Keyword(s):  
Cell Wall ◽  
Cell Wall Polysaccharides ◽  
Simulated Digestion ◽  
Actinidia Spp

scholarly journals Species-Specific Immunological Reactivities Depend on the Cell-Wall Organization of the Two Aspergillus, Aspergillus fumigatus and A. flavus

2021 ◽  
Vol 11 ◽  
Author(s):  
Sarah Sze Wah Wong ◽  
Lakshmi Prabha Venugopalan ◽  
Audrey Beaussart ◽  
Anupama Karnam ◽  
Mohammed Razeeth Shait Mohammed ◽  
...  
Keyword(s):  
Cell Wall ◽  
Aspergillus Fumigatus ◽  
Inflammatory Responses ◽  
Treatment Strategies ◽  
Specific Treatment ◽  
Antifungal Drugs ◽  
Cell Wall Polysaccharides ◽  
Superficial Infection ◽  
Species Specific

Although belong to the same genus, Aspergillus fumigatus is primarily involved in invasive pulmonary infection, whereas Aspergillus flavus is a common cause of superficial infection. In this study, we compared conidia (the infective propagules) of these two Aspergillus species. In immunocompetent mice, intranasal inoculation with conidia of A. flavus resulted in significantly higher inflammatory responses in the lungs compared to mice inoculated with A. fumigatus conidia. In vitro assays revealed that the dormant conidia of A. flavus, unlike A. fumigatus dormant conidia, are immunostimulatory. The conidial surface of A. fumigatus was covered by a rodlet-layer, while that of A. flavus were presented with exposed polysaccharides. A. flavus harbored significantly higher number of proteins in its conidial cell wall compared to A. fumigatus conidia. Notably, β-1,3-glucan in the A. flavus conidial cell-wall showed significantly higher percentage of branching compared to that of A. fumigatus. The polysaccharides ensemble of A. flavus conidial cell wall stimulated the secretion of proinflammatory cytokines, and conidial cell wall associated proteins specifically stimulated IL-8 secretion from the host immune cells. Furthermore, the two species exhibited different sensitivities to antifungal drugs targeting cell wall polysaccharides, proposing the efficacy of species-specific treatment strategies. Overall, the species-specific organization of the conidial cell wall could be important in establishing infection by the two Aspergillus species.

scholarly journals Retention of Primary Bile Acids by Lupin Cell Wall Polysaccharides Under In Vitro Digestion Conditions

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 2117 ◽  
Author(s):  
Naumann ◽  
Schweiggert-Weisz ◽  
Haller ◽  
Eisner
Keyword(s):  
Cell Wall ◽  
Bile Acid ◽  
Bile Acids ◽  
Molecular Interactions ◽  
Enterohepatic Circulation ◽  
In Vitro Digestion ◽  
Adsorptive Capacity ◽  
Cell Wall Polysaccharides ◽  
Dietary Fibres

Interference of dietary fibres with the enterohepatic circulation of bile acids is proposed as a mechanism for lowering cholesterol. We investigated how lupin hull and cotyledon dietary fibres interact with primary bile acids using an in vitro model under simulated upper gastrointestinal conditions. Cell wall polysaccharides were isolated and extracted to separate pectin-like, hemicellulosic, and lignocellulosic structures. Lupin hull consisted mainly of structural components rich in cellulose. The viscosity of the in vitro digesta of lupin hull was low, showing predominantly liquid-like viscoelastic properties. On the other hand, lupin cotyledon fibre retarded bile acid release due to increased viscosity of the in vitro digesta, which was linked with high contents of pectic polymers forming an entangled network. Molecular interactions with bile acids were not measured for the hull but for the cotyledon, as follows: A total of 1.29 µmol/100 mg DM of chenodesoxycholic acids were adsorbed. Molecular interactions of cholic and chenodesoxycholic acids were evident for lignin reference material but did not account for the adsorption of the lupin cotyledon. Furthermore, none of the isolated and fractionated cell wall materials showed a significant adsorptive capacity, thus disproving a major role of lupin cell wall polysaccharides in bile acid adsorption.

In vitro microbial digestion of straw cell wall polysaccharides in response to supplementation with different sources of carbohydrates

2000 ◽  
Vol 51 (3) ◽  
pp. 393 ◽  
Author(s):  
A. Barrios Urdaneta ◽  
M. Fondevila ◽  
J. Balcells ◽  
C. Dapoza ◽  
C. Castrillo
Keyword(s):  
Cell Wall ◽  
Bacterial Adhesion ◽  
Cell Walls ◽  
Cellulase Activity ◽  
Cellulolytic Bacteria ◽  
Cell Wall Polysaccharides ◽  
Carbohydrate Supplementation ◽  
Purine Bases ◽  
Different Sources

The effect of carbohydrate supplementation on microbial fibre digestion was studied in vitro, by measuring the disappearance of cell wall monosaccharides, bacterial adhesion (mmol purine bases per g residue), and total (per g residue) and bacterial (per mmol purine bases) polysaccharidase activity. Straw cell walls (CW, 0.5% w/v) were cultured in medium supplemented with (0.275% w/v) or without starch, a sugar mixture, or pectin. Supplementation with these constituents did not cause a drop in pH below 6.1, and increased all parameters investigated with the exception of bacterial polysaccharidase activity, which was higher for CW cultures, suggesting a higher proportion of fibrolytic bacteria in the adherent population. By comparison with starch and sugar, pectin supplementation resulted in a lower proportion of residual sugars remaining from cell walls after 60 and 72 h (P < 0.05), which resulted in greater bacterial adhesion after 8 and 12 h (P < 0.05) and higher total cellulase activity after 8 h (P < 0.01). This was perhaps because pectin may cover particle surfaces, protecting the digestive area from external factors, or may act as a substrate for cellulolytic bacteria. The lack of differences in bacterial enzymatic activities suggests the absence of qualitative or quantitative differences in the adherent fibrolytic population.

scholarly journals The role of L-arabinose metabolism for Escherichia coli O157:H7 in edible plants

2021 ◽  
Author(s):  
Louise Emma Crozier ◽  
Jacqueline Marshall ◽  
Ashleigh Holmes ◽  
Kathryn Wright ◽  
Yannick Rossez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Escherichia Coli O157 ◽  
In Planta ◽  
Cell Wall Degrading Enzymes ◽  
Cell Wall Polysaccharides ◽  
E Coli ◽  
Degrading Enzymes ◽  
Food Borne

Arabinose is a major plant aldopentose in the form of arabinans complexed in cell wall polysaccharides or glycoproteins (AGP), but comparatively rare as a monosaccharide. L-arabinose is an important bacterial metabolite, accessed by pectolytic microorganisms such as Pectobacterium atrosepticum via pectin and hemicellulose degrading enzymes. However, not all plant-associated microbes encode cell wall degrading enzymes, yet can metabolise L-arabinose, raising questions about their use of and access to the glycan in plants. Therefore, we examined L-arabinose metabolism in the food-borne pathogen Escherichia coli O157:H7 (isolate Sakai) during its colonisation of plants. L-arabinose metabolism (araBA) and transport (araF) genes were activated at 18 C in vitro by L-arabinose and expressed over prolonged periods in planta. Although deletion of araBAD did not impact the colonisation ability of E. coli O157:H7 (Sakai) on plants, araA was induced on exposure to spinach cell wall polysaccharides. Furthermore, debranched and arabinan oligosaccharides induced ara metabolism gene expression in vitro, and stimulated modest proliferation, while immobilised pectin did not. Thus, E. coli O157:H7 (Sakai) can utilise pectin/AGP-derived L-arabinose as a metabolite, but differs fundamentally in ara gene organisation, transport and regulation from the related pectinolytic species P. atrosepticum, reflective of distinct plant-associated lifestyles.

scholarly journals Structural Properties and Macrophage Activation of Cell Wall Polysaccharides from the Fruiting Bodies of Hericium erinaceus

Polymers ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 850
Author(s):  
Di Wu ◽  
Shan Yang ◽  
Chuan Tang ◽  
Yanfang Liu ◽  
Qiaozhen Li ◽  
...  
Keyword(s):  
Cell Wall ◽  
Structural Properties ◽  
Fruiting Body ◽  
Macrophage Activation ◽  
Water Soluble ◽  
Fruiting Bodies ◽  
Cell Wall Polysaccharides ◽  
Hericium Erinaceus ◽  
Soluble Cell

In this study, water-soluble and alkali-soluble cell wall polysaccharides were obtained from fruiting body extracted residual micropowders of Hericium erinaceus, harvested at seven different growing stages. The structural properties and in vitro immunity activities of cell wall polysaccharides extracted successively by hot water and sodium hydroxide solution were studied, and the results indicated that the yield and content of polysaccharides increased during the reproductive growth stage and decreased with the maturity of the fruiting body. Water-soluble cell wall polysaccharides mainly composed of glucose and galactose at a molar ratio of 3.4–14:1.0, and also contained a small ratio of glucuronic acid. The alkali-soluble cell wall polysaccharides were glucans with lower molecular weight and higher macrophage activation activity in vitro than water-soluble ones. Our findings suggest that the growth stages (H4 and H5) are suitable for harvesting H. erinaceus fruiting bodies with higher cell wall polysaccharide yield and functional benefits.

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