Coupling 6-chloro-3-methyluracil with copper: structural features, theoretical analysis, and biofunctional properties

2021 ◽  
Vol 50 (38) ◽  
pp. 13533-13542
Author(s):  
Brajesh Kumar ◽  
Tushar Das ◽  
Subhadeep Das ◽  
Waldemar Maniukiewicz ◽  
Dmytro S. Nesterov ◽  
...  

A unique copper(ii) complex with 6-chloro-3-methyluracil was assembled and fully characterized, revealing a notable antibacterial and antioxidant activity.

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2888
Author(s):  
Carmen M. S. Ambrosio ◽  
Gloria L. Diaz-Arenas ◽  
Leidy P. A. Agudelo ◽  
Elena Stashenko ◽  
Carmen J. Contreras-Castillo ◽  
...  

Essential oils (EOs) from Citrus are the main by-product of Citrus-processing industries. In addition to food/beverage and cosmetic applications, citrus EOs could also potentially be used as an alternative to antibiotics in food-producing animals. A commercial citrus EO—Brazilian Orange Terpenes (BOT)—was fractionated by vacuum fractional distillation to separate BOT into various fractions: F1, F2, F3, and F4. Next, the chemical composition and biological activities of BOT and its fractions were characterized. Results showed the three first fractions had a high relative amount of limonene (≥10.86), even higher than the whole BOT. Conversely, F4 presented a larger relative amount of BOT’s minor compounds (carvone, cis-carveol, trans-carveol, cis-p-Mentha-2,8-dien-1-ol, and trans-p-Mentha-2,8-dien-1-ol) and a very low relative amount of limonene (0.08–0.13). Antibacterial activity results showed F4 was the only fraction exhibiting this activity, which was selective and higher activity on a pathogenic bacterium (E. coli) than on a beneficial bacterium (Lactobacillus sp.). However, F4 activity was lower than BOT. Similarly, F4 displayed the highest antioxidant activity among fractions (equivalent to BOT). These results indicated that probably those minor compounds that detected in F4 would be more involved in conferring the biological activities for this fraction and consequently for the whole BOT, instead of the major compound, limonene, playing this role exclusively.


Author(s):  
FAVIAN BAYAS-MOREJON ◽  
ANGELICA TIGRE ◽  
RIVELINO RAMON ◽  
DANILO YANEZ

Objective: The increase in chronic and degenerative diseases and the use of synthetic antioxidants such as (butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT)) are being restricted because they can be considered carcinogenic. Therefore, there is a growing interest in the search for natural antioxidants, especially from plants, due to their content in different bioactive compounds, such as antioxidants and antimicrobials. To evaluate the antibacterial and antioxidant activity of Baccharislatifolia extracts. Methods: For the determination of the antimicrobial activity of extracts of leaves, root, stem and flowers of Baccharislatifolia (Bl), the disk plate diffusion method was used, the strains of Listeria, Salmonella and E. coli were studied; antibiotics Penicillin G and Ciprofloxacin were the controls. For the antioxidant activity, a solution of H2O2 (Abs at 230 nm) was prepared in Potassium Phosphate Monobasic-Sodium Hydroxide buffer. Results: The antimicrobial activity against Listeria and Salmonella, showed that the extracts of leaves and flowers were more effective with inhibition zones>15 mm and>20 mm respectively. In front of E. coli, the extracts of flowers and stem were the best with zones>7.0 mm. Antibiotics studied inhibited the development of Listeria and Salmonella. However, E. coli isolates were resistant. In the antioxidant activity, the flower extract of Bl in 60 mg/ml presents a higher effect with 47.25%. Conclusion: Bl extracts from leaves and flowers were more efficient both in their antimicrobial and antioxidant capacity.


2020 ◽  
Vol 2 (1) ◽  
pp. 43

Huge amounts of feathers are discarded as wastage, and it has always been environmentally concerned as they are difficult to destroy. Feather establishes over 90% protein, which gives it a rigid structure. Biotechnological techniques can help to degrade the feathers and use as biofertilizer. The best strategy is by utilizing keratinase producing keratinolytic microorganisms from the poultry waste to deteriorate the feathers. The poultry sample was collected at the local poultry farm. Using skimmed milk agar, enriched proteolytic bacteria were isolated, and the colony morphology assessed. The isolated bacteria were assessed for keratinolytic ability by using carbon and nitrogen sources. Liquid protein hydrolysate (LPH) was prepared and added as fertilizer to determine the growth effect on Capsicum annum. The antibacterial and antioxidant activity was assessed. The isolated Proteus sp. from the poultry waste has the ability to disintegrate the feathers completely on the 10th day. The enzymatic activity from Proteus sp. was observed increased with the presence of fructose (1.435 U/mL) and yeast extract (2.045 U/mL). The optimum temperature was at 40 °C (0.664 U/mL), pH value 7 (0.871 U/mL), and feather concentration at 1.5% (1.2 U/mL). LPH promoted the growth of Capsicum annum and increased total chlorophyll content (5.7341mg/g) in test plants. The antimicrobial activity displayed that Escherichia coli is susceptible to LPH, and also increased antioxidant activity was demonstrated in the test plants. Thus, the addition of liquid protein hydrolysate exhibited that it has the capability to aid plant development.


2012 ◽  
Vol 5 (4) ◽  
pp. 221-225
Author(s):  
N. Hemashenpagam ◽  
◽  
S. Aswathy ◽  
T. Selvaraj ◽  
A. Panneerselvam ◽  
...  

Polymers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2092 ◽  
Author(s):  
Miaomiao Xu ◽  
Chao Wang ◽  
Gaojin Lyu ◽  
Lei Zhong ◽  
Liyuan Yang ◽  
...  

Xylose residue (XR), after diluted acid treatment of corncob, consists of cellulose and lignin. However, structural changes of XR lignin have not been investigated comprehensively, and this has seriously hindered the efficient utilization of lignin. In this study, corncob milled wood lignin (CC MWL), and xylose residue milled wood lignin (XR MWL) were isolated according to the modified milled wood lignin (MWL) method. The structural features of two lignin fractions were thoroughly investigated via fourier transform infrared spectroscopy (FTIR), gel permeation chromatography (GPC), thermogravimetric analysis (TGA) and two dimensional nuclear magnetic resonance (2D NMR) spectroscopy techniques. XR MWL with higher yield and lower bound carbohydrate contents presented more phenolic OH contents than CC MWL due to partial cleavage of β-O-4. Furthermore, the molecular weights of XR MWL were increased, possibly because of condensation of the lignin during the xylose production. A study on antioxidant activity showed that XR lignin had better radical scavenging ability than that of 2,6-Di-tert-butyl-4-methyl-phenol (BHT) and CC MWL. The results suggested that the lignin in xylose residue, showing great antioxidant properties, has potential applications in food additives.


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