Activatable fluorescent probes for in situ imaging of enzymes

2022 ◽  
Author(s):  
Xiaofeng Wu ◽  
Rui Wang ◽  
Nahyun Kwon ◽  
Huimin Ma ◽  
Juyoung Yoon
Keyword(s):  
Fluorescent Probes ◽  
Design Strategies ◽  
In Situ Imaging

This tutorial review provides an overview of activatable fluorescent probes for in situ imaging of enzymes, including design strategies, sensing mechanisms, and bioapplications.

In Situ Imaging of Zinc with Synthetic Fluorescent Probes

2019 ◽  
pp. 365-388
Author(s):  
Jiyao Yu ◽  
Christoph J. Fahrni
Keyword(s):  
Fluorescent Probes ◽  
In Situ Imaging

scholarly journals A de novo strategy to develop NIR precipitating fluorochrome for long-term in situ cell membrane bioimaging

2021 ◽  
Vol 118 (8) ◽  
pp. e2018033118
Author(s):  
Ke Li ◽  
Yifan Lyu ◽  
Yan Huang ◽  
Shuai Xu ◽  
Hong-Wen Liu ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
De Novo ◽  
Membrane Surface ◽  
In Situ Monitoring ◽  
Fluorescence Signal ◽  
In Situ Imaging

Cell membrane–targeted bioimaging is a prerequisite for studying the roles of membrane-associated biomolecules in various physiological and pathological processes. However, long-term in situ bioimaging on the cell membrane with conventional fluorescent probes leads to diffusion into cells from the membrane surface. Therefore, we herein proposed a de novo strategy to construct an antidiffusion probe by integrating a fluorochrome characterized by strong hydrophobicity and low lipophilicity, with an enzyme substrate to meet this challenge. This precipitating fluorochrome HYPQ was designed by conjugating the traditionally strong hydrophobic solid-state fluorochrome 6-chloro–2-(2-hydroxyphenyl) quinazolin-4(3H)-one (HPQ) with a 2-(2-methyl–4H-chromen–4-ylidene) malononitrile group to obtain closer stacking to lower lipophilicity and elongate emission to the far-red to near-infrared wavelength. As proof-of-concept, the membrane-associated enzyme γ-glutamyltranspeptidase (GGT) was selected as a model enzyme to design the antidiffusion probe HYPQG. Then, benefiting from the precipitating and stable signal properties of HYPQ, in situ imaging of GGT on the membrane was successfully realized. Moreover, after HYPQG was activated by GGT, the fluorescence signal on the cell membrane remained unchanged, with incubation time even extending to 6 h, which is significant for in situ monitoring of enzymatic activity. In vivo testing subsequently showed that the tumor region could be accurately defined by this probe after long-term in situ imaging of tumor-bearing mice. The excellent performance of HYPQ indicates that it may be an ideal alternative for constructing universal antidiffusion fluorescent probes, potentially providing an efficient tool for accurate imaging-guided surgery in the future.

Development of in situ Imaging Probe for Surgical Operation of Deep Brain Stimulation

2011 ◽  
Vol 131 (12) ◽  
pp. 427-428
Author(s):  
Toshihiko Noda ◽  
Pan Yi-Li ◽  
Ayato Tagawa ◽  
Takuma Kobayashi ◽  
Kiyotaka Sasagawa ◽  
...  
Keyword(s):  
Deep Brain Stimulation ◽  
Brain Stimulation ◽  
Surgical Operation ◽  
Imaging Probe ◽  
Deep Brain ◽  
In Situ Imaging

Single cell capture, isolation, and long‐term in‐situ imaging using quantitative self‐interference spectroscopy

2021 ◽  
Author(s):  
Rongxin Fu ◽  
Ya Su ◽  
Ruliang Wang ◽  
Xue Lin ◽  
Xiangyu Jin ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Capture ◽  
In Situ Imaging

scholarly journals Polarity-active NIR probes with strong two-photon absorption and ultrahigh binding affinity of insulin amyloid fibrils

2021 ◽  
Author(s):  
Li Li ◽  
Zheng Lv ◽  
Zhongwei Man ◽  
Zhenzhen Xu ◽  
YuLing Wei ◽  
...  
Keyword(s):  
Neurodegenerative Diseases ◽  
Binding Affinity ◽  
Fluorescent Probes ◽  
Amyloid Fibrils ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Medical Diagnostic

Amyloid fibrils are associated with many neurodegenerative diseases. In-situ and in-vivo visualization of amyloid fibrils is important for medical diagnostic and requires fluorescent probes with both excitation and emission wavelengths in...

Persistent luminescent nanoparticles for super-long time in vivo and in situ imaging with repeatable excitation

2014 ◽  
Vol 145 ◽  
pp. 838-842 ◽  
Author(s):  
Meng Sun ◽  
Zhan-Jun Li ◽  
Chun-Lin Liu ◽  
Hai-Xia Fu ◽  
Jiang-Shan Shen ◽  
...  
Keyword(s):  
Long Time ◽  
Luminescent Nanoparticles ◽  
In Situ Imaging
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