Experimental studies on combined production of CH4 and safe long-term storage of CO2 in the form of solid hydrate in sediment

2021 ◽  
Author(s):  
Navid Saeidi ◽  
Derek Dunn-Rankin ◽  
Bjørn Kvamme ◽  
Yu-Chien Chien
Keyword(s):  
Liquid State ◽  
Methane Hydrate ◽  
Experimental Studies ◽  
Long Term Storage ◽  
Term Storage ◽  
Fast Mechanism

34 mole% CO2 is substituted into the methane hydrate by adding N2 from injection fluid for increasing the performance. Liquid state is the fast mechanism for replacing in situ CH4 with CO2 and involves the formation of a new CO2 hydrate.

scholarly journals Effects of Long-Term Storage on the Detection of Proteins, DNA, and mRNA in Tissue Microarray Slides

2011 ◽  
Vol 59 (12) ◽  
pp. 1113-1121 ◽  
Author(s):  
Christina Karlsson ◽  
Mats G. Karlsson
Keyword(s):  
Gene Copy Number ◽  
Tissue Microarrays ◽  
Storage Conditions ◽  
Gene Copy ◽  
Histological Techniques ◽  
Long Term Storage ◽  
Term Storage ◽  
Formalin Fixed

Storage of tissue slides has been claimed to induce dramatically reduced antigen detection particularly for immunohistochemistry (IHC). With tissue microarrays, the necessity to serially cut blocks in order to obtain as much material as possible is obvious. The presumed adverse effect of storage might hamper such an approach. The authors designed an experimental setting consisting of four different storage conditions with storage time of tissue slides of up to 1 year. Detection of proteins, DNA, and mRNA was performed using IHC and in situ hybridization techniques. Slight but significant changes in IHC occurred over time. The most important factor is the primary antibody used: four showed no significant changes, whereas limited decreases in 8 antibodies could be detected by image analysis. Whether the antigen was nuclear or cytoplasmic/membranous did not matter. No major differences between different storage conditions could be shown, but storage at 4C was overall the best procedure. Furthermore, gene copy number aberrations, chromosomal translocations, and the presence of mRNA could be detected on slides stored up to 1 year. In conclusion, in tissues optimally formalin fixed and using modern histological techniques, only minute changes in tissue antigenicity are induced by long-term storage.

An Experimental Approach to the Function of Classic Maya Chultuns

1971 ◽  
Vol 36 (3) ◽  
pp. 322-335 ◽  
Author(s):  
Dennis E. Puleston
Keyword(s):  
Experimental Approach ◽  
Experimental Studies ◽  
Water Storage ◽  
Food Storage ◽  
Food Crop ◽  
Traditional Foods ◽  
Long Term Storage ◽  
Brosimum Alicastrum ◽  
Term Storage

AbstractExperimental techniques have provided an exciting breakthrough for the functional analysis of Maya chultuns. While deep cistern-like chultuns, common at certain sites in the northern lowlands, have been shown to be functional for water storage, smaller lateral-chambered chultuns characteristic of certain parts of the southern lowlands probably had a very different function. Excavation and examination of the latter features, in light of a whole range of possibilities, suggest that they were constructed to be used for food storage. Experimental studies, however, reveal them to be unsuitable for the storage of most traditional foods, including maize. At least one local food crop, the seed of the ramon (Brosimum alicastrum, Moraceae), appears to be ideally suited for long-term storage under these conditions. Chambers constructed beneath platforms in the northern lowlands may have been used for the storage of maize. A need for more experimental work is indicated.

Population genetics and conservation of the critically endangered Clematis acerifolia (Ranunculaceae)

2005 ◽  
Vol 83 (10) ◽  
pp. 1248-1256 ◽  
Author(s):  
J. López-Pujol ◽  
F.-M. Zhang ◽  
S. Ge
Keyword(s):  
Genetic Diversity ◽  
Population Genetic ◽  
Critically Endangered ◽  
Ex Situ ◽  
Genetic Structuring ◽  
Long Term Storage ◽  
Hardy Weinberg Equilibrium ◽  
Term Storage

Allozyme electrophoresis was used to evaluate the levels of genetic diversity and population genetic structure of the critically endangered Clematis acerifolia Maximowicz (Ranunculaceae), a narrow endemic species in China. On the basis of variation at 19 putative loci in nine populations covering the entire distribution of this species, low values of genetic diversity were detected (P = 20.5%, A = 1.27, and He = 0.072). A significant deficiency of heterozygotes was found in all populations. Most loci showed deviations from the Hardy–Weinberg equilibrium, probably as a result of population genetic structuring. The high genetic divergence among populations (FST = 0.273) can be interpreted as an effect of the extinction of local populations and genetic drift within extant populations, and has probably been enhanced by habitat fragmentation in recent decades. Threats to this species are mainly anthropogenic (road works, construction of holiday resorts, and extraction activities), although stochastic risks cannot be ignored. Therefore, to preserve extant genetic variation of C. acerifolia, in situ strategies, such as the preservation of its habitat or at least the most diverse populations, and ex situ measures, such as the collection and long-term storage of seeds, should be adopted.

scholarly journals Тhe quality of potatoes affects consumer properties of processed foods

2021 ◽  
pp. 99-103
Author(s):  
A. A. Molyavko ◽  
A. V. Marukhlenko ◽  
N. P. Borisova
Keyword(s):  
Mineral Nutrition ◽  
Dry Matter ◽  
Reducing Sugars ◽  
Starch Content ◽  
Experimental Studies ◽  
Long Term Storage ◽  
Almost All ◽  
Term Storage

Experimental studies have shown that with an increased level of mineral nutrition (N120P180K240) all varieties increased the yield (by 1.7–4.1 tons/ha, or by 10–25%), while the most productive varieties being Bryansky Nadezhny, Bryanskaya Novinka, Slava Bryanshchiny (21.9–22.9 tons/ha). In terms of dry substances content almost all varieties met the requirements of processing, which called for for a dry substance level in tubers of at least 20–24%. With an increase in the dose of fertilizers the amount of starch and dry matter in tubers decreased. On an average background, the starch content compared to the control decreased by 0.7–1.1%, dry substances — by 0.7– 1.2%, on an increased background respectively — by 1.2–1.7%, dry substances — by 1.4–2.7%. The taste of potatoes on an increased background of fertilizers worsened regardless of variety. In terms of darkening of the flesh, raw cleaned tubers of all varieties are not suitable for long-term storage (for example, for 24 hours), while boiled tubers of all varieties did not reduce the quality. Growing potatoes on an increased background of mineral nutrition increased the darkening of raw tubers of the studied varieties compared to control. In the studied varieties the content of reducing sugars in many cases exceeded the amount allowed for the manufacture of crispy potatoes, and therefore its quality was reduced, mainly due to the color of the slices. Almost all varieties gave puree of good quality. An increase in the background of fertilizers slightly worsened the consistency of puree, which reduced the overall estimate.

scholarly journals Assessing the efficiency of a “buried wall” barrier in the establishment of near-surface long-term storage and disposal facilities for RW

2021 ◽  
Vol 14 (1) ◽  
pp. 96-105
Author(s):  
V. V. Suskin ◽  
◽  
I. V. Kapyrin ◽  
F. V. Grigorev ◽  
◽  
...  
Keyword(s):  
Near Surface ◽  
Transfer Processes ◽  
Long Term Storage ◽  
Mass Transfer Processes ◽  
High Degree ◽  
The Impact ◽  
Term Storage

The article evaluates the impact of a “buried wall” barrier on the long-term safety during the long-term storage1 or in-situ disposal of nuclear legacy facilities, in particular, industrial reservoirs, as well as during the development of near-surface disposal facilities for radioactive waste (RWDF). For assessment purposes, filtration and mass transfer processes have been numerically modelled in the GeRa code based on a case study of a reference near-surface facility. The study explores in which way the available covering screen affects the dynamics of contaminant spread. It evaluates the sensitivity of the results to the dispersion parameter commonly characterized by a high degree of uncertainty.

scholarly journals Ensuring long storage of raw sugar products using electrophysical installation

2021 ◽  
pp. 56-61
Author(s):  
M. A. Yakiyayeva ◽  
A. N. Zhakatayeva ◽  
A. I. Iztayev ◽  
M. M. Маyемеrоv ◽  
B Zh. Muldabekova
Keyword(s):  
Shelf Life ◽  
Experimental Studies ◽  
Sugar Beets ◽  
Sugar Factory ◽  
Long Term Storage ◽  
Raw Sugar ◽  
Term Storage ◽  
Optimal Regimen ◽  
Long Storage

The article examined the efficiency of using an electrophysical installation to ensure the long-term storage of raw sugar products. Experimental studies of sugar beets of the second degree of infection of the Koksu sugar factory. As a result, it was found that in order to increase the shelf life of sugar products, the most optimal regimen is treatment with ozone with a concentration of 8 g/m3 for 20 minutes and an overpressure of 3 or 5 ati.

scholarly journals RISE microscopy for evaluation of compounded disinfection products

2018 ◽  
Vol 2018 (6) ◽  
pp. 30-33
Author(s):  
Олег Татарчук ◽  
Oleg Tatarchuk
Keyword(s):  
Emergency Preparedness ◽  
Comprehensive Evaluation ◽  
Chemical Characteristics ◽  
Pharmaceutical Samples ◽  
Long Term Storage ◽  
In Situ Generation ◽  
Non Destructive ◽  
Term Storage

Multicomponent powdered disinfection products with in situ generation of biocidal substances are stable in long-term storage and therefore well suited for emergency preparedness stockpiling to use in outbreaks of infectious diseases. Certain structural and chemical characteristics of the powdered biocidal mixtures might alter their efficacy, safety, or handling properties. The RISE microscopy is a new non-destructive method for comprehensive evaluation of pharmaceutical samples by combining confocal Raman imaging with scanning electron microscopy. The RISE microscopy can be used for evaluation of compounded disinfection products.

scholarly journals Recycling and Long-Term Storage of Fluorescence In Situ Hybridization Slides

2014 ◽  
Vol 141 (3) ◽  
pp. 374-380 ◽  
Author(s):  
Susumu Wakai ◽  
Yasuo Shibuki ◽  
Karin Yokozawa ◽  
Shoko Nakamura ◽  
Yuko Adegawa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Long Term Storage ◽  
Term Storage
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