Protein Crystallisation Facilitated by Silica Particles to Compensate the Adverse Impact from Protein Impurity

CrystEngComm ◽

10.1039/d1ce00983d ◽

2021 ◽

Author(s):

Xiaoyu Li ◽

Jerry Y. Y. Heng

Keyword(s):

Silica Particles ◽

Target Protein ◽

Adverse Impact ◽

Protein Mixture

In this study, silica particles were used to improve target protein batch crystallisation from a binary protein mixture at 5 mL scale. Lysozyme (40 mg/mL) was used as the target...

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Selective purification and chemical labeling of a target protein on ruthenium photocatalyst-functionalized affinity beads

Chemical Communications ◽

10.1039/c7cc01595j ◽

2017 ◽

Vol 53 (35) ◽

pp. 4838-4841 ◽

Cited By ~ 14

Author(s):

Michihiko Tsushima ◽

Shinichi Sato ◽

Hiroyuki Nakamura

Keyword(s):

Target Protein ◽

Chemical Labeling ◽

Protein Mixture ◽

Affinity Beads

Simultaneous selective purification and chemical labeling of a target protein were achieved on the surface of affinity beads functionalized with a ruthenium photocatalyst and a ligand in a protein mixture.

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Quantification of Silica Uptake by Alveolar Macrophages - An Emperical Scanning Electron Microprobe Method

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100054297 ◽

1982 ◽

Vol 40 ◽

pp. 332-333

Author(s):

V. V. Damiano ◽

R. P. Daniele ◽

H. T. Tucker ◽

J. H. Dauber

Keyword(s):

Quantitative Analysis ◽

Alveolar Macrophages ◽

Bulk Sample ◽

Silica Particles ◽

Empirical Method ◽

Phagocytic Cells ◽

Calibration Standards ◽

X Ray ◽

Accurate Quantitative Analysis ◽

Scanning Electron

An important example of intracellular particles is encountered in silicosis where alveolar macrophages ingest inspired silica particles. The quantitation of the silica uptake by these cells may be a potentially useful method for monitoring silica exposure. Accurate quantitative analysis of ingested silica by phagocytic cells is difficult because the particles are frequently small, irregularly shaped and cannot be visualized within the cells. Semiquantitative methods which make use of particles of known size, shape and composition as calibration standards may be the most direct and simplest approach to undertake. The present paper describes an empirical method in which glass microspheres were used as a model to show how the ratio of the silicon Kα peak X-ray intensity from the microspheres to that of a bulk sample of the same composition correlated to the mass of the microsphere contained within the cell. Irregular shaped silica particles were also analyzed and a calibration curve was generated from these data.

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Is Retest Bias Biased?

Journal of Personnel Psychology ◽

10.1027/1866-5888/a000149 ◽

2016 ◽

Vol 15 (2) ◽

pp. 45-54 ◽

Cited By ~ 4

Author(s):

Jason G. Randall ◽

Anton J. Villado ◽

Christina U. Zimmer

Keyword(s):

Sex Differences ◽

Emotional Stability ◽

Adverse Impact ◽

Mental Ability ◽

Ability Test ◽

General Mental Ability ◽

Psychological Mechanisms

Abstract. The purpose of this study was to test for race and sex differences in general mental ability (GMA) retest performance and to identify the psychological mechanisms underlying these differences. An initial and retest administration of a GMA assessment separated by a six-week span was completed by 318 participants. Contrary to our predictions, we found that race, sex, and emotional stability failed to moderate GMA retest performance. However, GMA assessed via another ability test and conscientiousness both partially explained retest performance. Additionally, we found that retesting may reduce adverse impact ratios by lowering the hiring threshold. Ultimately, our findings reinforce the need for organizations to consider race, sex, ability, and personality when implementing retesting procedures.

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