Visualization of lipophagy using a supramolecular FRET pair

2021 ◽  
Author(s):  
Ara Lee ◽  
Meng Li ◽  
Young Ho Ko ◽  
Sanghwang Park ◽  
Jongcheol Seo ◽  
...  
Keyword(s):  
Boron Dipyrromethene ◽  
Fret Pair

A rationally designed supramolecular FRET pair consisting of cyanine3-cucurbit[7]uril (Cy3-CB[7]) and boron-dipyrromethene 630/650-adamantylammonium (BDP-AdA) can be used to visualize organelle-specific autophagy events. The intracellular accumulations of Cy3-CB[7] in lysosome and...

Using FRET to Measure the Time it Takes for a Cell to Destroy a Virus

2019 ◽  
Author(s):  
Candace E. Benjamin ◽  
Zhuo Chen ◽  
Olivia Brohlin ◽  
Hamilton Lee ◽  
Stefanie Boyd ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Rhodamine B ◽  
Virus Like Particle ◽  
A Cell ◽  
Viral Nanoparticles ◽  
The Stability ◽  
Open Question ◽  
Viral Surface ◽  
Fret Pair

<div><div><div><p>The emergence of viral nanotechnology over the preceding two decades has created a number of intellectually captivating possible translational applications; however, the in vitro fate of the viral nanoparticles in cells remains an open question. Herein, we investigate the stability and lifetime of virus-like particle (VLP) Qβ - a representative and popular VLP for several applications - following cellular uptake. By exploiting the available functional handles on the viral surface, we have orthogonally installed the known FRET pair, FITC and Rhodamine B, to gain insight of the particle’s behavior in vitro. Based on these data, we believe VLPs undergo aggregation in addition to the anticipated proteolysis within a few hours of cellular uptake.</p></div></div></div>

scholarly journals Ready Access to Molecular Rotors Based on Boron Dipyrromethene Dyes-Coumarin Dyads Featuring Broadband Absorption

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 781
Author(s):  
Ernesto Enríquez-Palacios ◽  
Teresa Arbeloa ◽  
Jorge Bañuelos ◽  
Claudia I. Bautista-Hernández ◽  
José G. Becerra-González ◽  
...  
Keyword(s):  
Molecular Rotors ◽  
Fluorescence Signal ◽  
Access Method ◽  
Broadband Absorption ◽  
Boron Dipyrromethene ◽  
Conformational Freedom ◽  
Meso Position ◽  
Photophysical Study ◽  
Ready Access

Herein we report on a straightforward access method for boron dipyrromethene dyes (BODIPYs)-coumarin hybrids linked through their respective 8- and 6- positions, with wide functionalization of the coumarin fragment, using salicylaldehyde as a versatile building block. The computationally-assisted photophysical study unveils broadband absorption upon proper functionalization of the coumarin, as well as the key role of the conformational freedom of the coumarin appended at the meso position of the BODIPY. Such free motion almost suppresses the fluorescence signal, but enables us to apply these dyads as molecular rotors to monitor the surrounding microviscosity.

Boron dipyrromethene fluorophore based fluorescence sensor for the selective imaging of Zn(ii) in living cells

2005 ◽  
Vol 3 (8) ◽  
pp. 1387 ◽  
Author(s):  
Yunkou Wu ◽  
Xiaojun Peng ◽  
Binchen Guo ◽  
Jiangli Fan ◽  
Zhichao Zhang ◽  
...  
Keyword(s):  
Living Cells ◽  
Fluorescence Sensor ◽  
Boron Dipyrromethene

Emission of boron dipyrromethene dyes through energy transfer to their S2 state from polysilane S1 state

2012 ◽  
Vol 94 (2) ◽  
pp. 183-186 ◽  
Author(s):  
Qiuhong Wang ◽  
Hua Lu ◽  
Lizhi Gai ◽  
Weifeng Chen ◽  
Guoqiao Lai ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Boron Dipyrromethene ◽  
S2 State

Boron-dipyrromethene based multi-anionic sensor and a specific cationic sensor for Fe3+

2014 ◽  
Vol 2 (28) ◽  
pp. 5576 ◽  
Author(s):  
Vellanki Lakshmi ◽  
Mangalampalli Ravikanth
Keyword(s):  
Boron Dipyrromethene
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