Biomineralized Zeolitic Imidazolate Framework-8 Nanoparticles Enable Polymerase/Endonuclease Synergetic Amplification Reaction in Living Cells for Sensitive MicroRNA Imaging

2021 ◽  
Author(s):  
Ke Zhang ◽  
Xiayun Le ◽  
Qiaoqin Yu ◽  
Juan Zhang ◽  
Dandan Wang ◽  
...  
Keyword(s):  
Living Cells ◽  
Zeolitic Imidazolate Framework ◽  
Amplification Reaction

In this work, we have developed a simple-prepared nanocomplex probe, which could implement polymerase and endonuclease synergetic amplification reaction in living cells by the biomineralized ZIF-8 NPs for intracellular miRNA...

Sensitive and sustained imaging of intracellular microRNA in living cells by a high biocompatible liposomal vehicle introduced isothermal symmetric exponential amplification reaction

2019 ◽  
Vol 55 (75) ◽  
pp. 11251-11254 ◽  
Author(s):  
Wen Yin ◽  
Jun Chen ◽  
Huihui Yang ◽  
Yanfei Zhang ◽  
Zong Dai ◽  
...  
Keyword(s):  
Living Cells ◽  
Highly Efficient ◽  
Amplification Reaction

A biocompatible liposome was fabricated to introduce highly efficient oligonucleotide amplification in living cells for the sensitive and sustained imaging of microRNA.

Enhancing intracellular microRNA imaging: a new strategy combining double-channel exciting single colour fluorescence with the target cycling amplification reaction

2018 ◽  
Vol 54 (93) ◽  
pp. 13131-13134 ◽  
Author(s):  
Keying Zhang ◽  
Shuting Song ◽  
Lin Yang ◽  
Qianhao Min ◽  
Xingcai Wu ◽  
...  
Keyword(s):  
Living Cells ◽  
New Strategy ◽  
Single Colour ◽  
Amplification Reaction ◽  
Double Channel

Enhancing microRNA imaging in living cells using double-channel exciting single colour fluorescence coupled with the target cycling amplification reaction.

scholarly journals DNA adsorption on nanoscale zeolitic imidazolate framework-8 enabling rational design of a DNA-based nanoprobe for gene detection and regulation in living cells

RSC Advances ◽  
2020 ◽  
Vol 10 (51) ◽  
pp. 31012-31021
Author(s):  
Shengmei Wang ◽  
Linqi Ouyang ◽  
Guiming Deng ◽  
Zhenzhen Deng ◽  
Shengfeng Wang
Keyword(s):  
Rational Design ◽  
Living Cells ◽  
Gene Detection ◽  
Dna Adsorption ◽  
Zeolitic Imidazolate Framework

Boosting DNA-based nanotheranostics for gene detection and regulation by ZIF-8.

Comparison of different zinc precursors for the construction of zeolitic imidazolate framework-8 artificial shells on living cells

Soft Matter ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 270-275 ◽  
Author(s):  
Wei Chen ◽  
Shu Kong ◽  
Meng Lu ◽  
Fangming Chen ◽  
Wen Cai ◽  
...  
Keyword(s):  
Cell Death ◽  
Zinc Acetate ◽  
Zinc Sulfate ◽  
Living Cells ◽  
Zinc Nitrate ◽  
Precipitation Method ◽  
Zeolitic Imidazolate Framework ◽  
Sulfate Lead

The robust zeolitic imidazolate framework-8 (ZIF-8) shell was formed on the living cells by an in situ precipitation method. Compared with zinc nitrate and zinc acetate, ZIF-8 formed from zinc sulfate lead to a higher percentage of cell death.

Multifunctional zeolitic imidazolate framework-8 for real-time monitoring ATP fluctuation in mitochondria during photodynamic therapy

Nanoscale ◽  
2020 ◽  
Vol 12 (29) ◽  
pp. 15663-15669
Author(s):  
Ke Wang ◽  
Manping Qian ◽  
Honglan Qi ◽  
Qiang Gao ◽  
Chengxiao Zhang
Keyword(s):  
Photodynamic Therapy ◽  
Real Time ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Zeolitic Imidazolate Framework

An ATP-responsive and fluorescent Zeolitic imidazolate framework-8 (ZIF-8) is synthesized for real-time monitoring mitochondrial ATP fluctuation in living cells during photodynamic therapy.

Monitoring Telomerase Activity in Living Cells with High Sensitivity Using Cascade Amplification Reaction-Based Nanoprobe

2019 ◽  
Vol 91 (20) ◽  
pp. 13143-13151 ◽  
Author(s):  
Huanhuan Fan ◽  
Huarong Bai ◽  
Qin Liu ◽  
Hang Xing ◽  
Xiao-Bing Zhang ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Telomerase Activity ◽  
Living Cells ◽  
Cascade Amplification ◽  
Amplification Reaction

Fluorescence ratio imaging of dynamic intracellular ionic signals

1988 ◽  
Vol 46 ◽  
pp. 42-43
Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian
Keyword(s):  
Binding Sites ◽  
Living Cells ◽  
Molecular Engineering ◽  
Ph Indicators ◽  
Highly Sensitive ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging ◽  
Technical Advances ◽  
Indicator Dyes ◽  
Fluorescein Dyes

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

Multimode light microscopy and fluorescence-based reagents as tools for the study of chemical and molecular dynamics of living cells and tissues

1992 ◽  
Vol 50 (1) ◽  
pp. 418-419
Author(s):  
D. L. Taylor
Keyword(s):  
Living Cells ◽  
Cellular Level ◽  
Molecular Techniques ◽  
Cellular Functions ◽  
Macromolecular Assemblies ◽  
Cell Functions ◽  
Molecular Events ◽  
Yield Information ◽  
Full Knowledge ◽  
Structural Methods

Cells function through the complex temporal and spatial interplay of ions, metabolites, macromolecules and macromolecular assemblies. Biochemical approaches allow the investigator to define the components and the solution chemical reactions that might be involved in cellular functions. Static structural methods can yield information concerning the 2- and 3-D organization of known and unknown cellular constituents. Genetic and molecular techniques are powerful approaches that can alter specific functions through the manipulation of gene products and thus identify necessary components and sequences of molecular events. However, full knowledge of the mechanism of particular cell functions will require direct measurement of the interplay of cellular constituents. Therefore, there has been a need to develop methods that can yield chemical and molecular information in time and space in living cells, while allowing the integration of information from biochemical, molecular and genetic approaches at the cellular level.

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