scholarly journals DNA adsorption on nanoscale zeolitic imidazolate framework-8 enabling rational design of a DNA-based nanoprobe for gene detection and regulation in living cells

RSC Advances ◽  
2020 ◽  
Vol 10 (51) ◽  
pp. 31012-31021
Author(s):  
Shengmei Wang ◽  
Linqi Ouyang ◽  
Guiming Deng ◽  
Zhenzhen Deng ◽  
Shengfeng Wang
Keyword(s):  
Rational Design ◽  
Living Cells ◽  
Gene Detection ◽  
Dna Adsorption ◽  
Zeolitic Imidazolate Framework

Boosting DNA-based nanotheranostics for gene detection and regulation by ZIF-8.

scholarly journals A rational design of a cancer-specific and lysosome-targeted fluorescence nanoprobe for glutathione imaging in living cells

2020 ◽  
Vol 1 (6) ◽  
pp. 1739-1744
Author(s):  
Hong Wang ◽  
Peisheng Zhang ◽  
Chonghua Zhang ◽  
Shu Chen ◽  
Rongjin Zeng ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
Rational Design ◽  
Living Cells ◽  
Dual Targeting ◽  
Fluorescence Nanoprobe

A dual-targeting (both cancer cell- and lysosome-targeting) fluorescence nanoprobe was rational designed and synthesized for the efficient imaging of lysosomal GSH in cancer cells.

scholarly journals A Universal Chemical Method for Rational Design of Protein-based Nanoreactors

2021 ◽  
Author(s):  
Mullapudi Mohan Reddy ◽  
Punita Bathla ◽  
Britto S. Sandanaraj
Keyword(s):  
Self Assembly ◽  
Rational Design ◽  
Chemical Method ◽  
Living Cells ◽  
Protein Labeling ◽  
Self Assembling ◽  
Naturally Occurring ◽  
General Chemical ◽  
Chemical Strategy ◽  
Artificial Protein

AbstractSelf-assembly of a monomeric protease to form a multi-subunit protein complex “proteasome” enables targeted protein degradation in living cells. The naturally occurring proteasomes serve as an inspiration and blueprint for the design of artificial protein-based nanoreactors. Here we disclose a general chemical strategy for the design of proteasome-like nanoreactors. Micelle-assisted protein labeling (MAPLab) technology along with the N-terminal bioconjugation strategy is utilized for the synthesis of a well-defined monodisperse self-assembling semi-synthetic protease. The designer protein is programmed to self-assemble into a proteasome-like nanostructure which preserves the functional properties of native protease.

scholarly journals Co-transcriptional RNA strand displacement circuits

2021 ◽  
Author(s):  
Samuel W Schaffter ◽  
Elizabeth Strychalski
Keyword(s):  
Simple Model ◽  
Rational Design ◽  
Biological Systems ◽  
Living Cells ◽  
Signaling Cascades ◽  
Strand Displacement ◽  
Model Driven ◽  
Displacement Reactions ◽  
Circuit Components

Engineered molecular circuits that process information in biological systems could address emerging human health and biomanufacturing needs. However, such circuits can be difficult to rationally design and scale. DNA-based strand displacement reactions have demonstrated the largest and most computationally powerful molecular circuits to date but are limited in biological systems due to the difficulty in genetically encoding components. Here, we develop scalable co-transcriptional RNA strand displacement (ctRSD) circuits that are rationally programmed via base pairing interactions. ctRSD addresses the limitations of DNA-based strand displacement circuits by isothermally producing circuit components via transcription. We demonstrate the programmability of ctRSD in vitro by implementing logic and amplification elements, and multi-layer signaling cascades. Further, we show ctRSD kinetics are accurately predicted by a simple model of coupled transcription and strand displacement, enabling model-driven design. We envision ctRSD will enable rational design of powerful molecular circuits that operate in biological systems, including living cells.

Molecular Beacons for Sensitive Gene Detection in Living Cells

2003 ◽  
Author(s):  
Andrew Tsurkas ◽  
Gang Bao
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Molecular Beacon ◽  
Point Mutations ◽  
Living Cells ◽  
Molecular Beacons ◽  
High Signal ◽  
Gene Detection ◽  
Real Time Imaging ◽  
Background Ratio

Real-time imaging of gene expression in living cells has the potential to significantly impact clinical and laboratory studies of cancer, including cancer diagnosis and analysis. Molecular beacons (MBs) provide a simple and promising tool for the detection of target mRNA as tumor markers due to their high signal-to-background ratio, and their improved specificity in detecting point mutations. However, the harsh intracellular environment does limit the sensitivity of MB-based gene detection. Specifically, MBs bound to target mRNAs cannot be distinguished from those degraded by nucleases, or opened due to non-specific interactions. To overcome this difficulty, we have developed a novel dual FRET molecular beacons approach in which a pair of molecular beacons, one with a donor fluorophore and a second with an acceptor fluorophore, hybridize to adjacent regions on the same target resulting in fluorescence resonance energy transfer (FRET). The detection of a FRET signal leads to a substantially increased signal-to-background ratio compared with that in single molecular beacon assays and enables discrimination between fluorescence due to specific probe/target hybridization and a variety of false-positive events. We have performed systematic in-solution and cellular studies of dual FRET molecular beacon and demonstrated that this new approach allows for real-time imaging of gene expression in living cells.

scholarly journals Self-Templating Synthesis of 3D Hierarchical NiCo2O4@NiO Nanocage from Hydrotalcites for Toluene Oxidation

Catalysts ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 352 ◽  
Author(s):  
Dongdong Wang ◽  
Shuangde Li ◽  
Yingchao Du ◽  
Xiaofeng Wu ◽  
Yunfa Chen
Keyword(s):  
Photoelectron Spectroscopy ◽  
Rational Design ◽  
Space Velocity ◽  
Acid Sites ◽  
Step Method ◽  
Zeolitic Imidazolate Framework ◽  
Toluene Oxidation ◽  
Synthetic Strategy ◽  
Temperature Programmed ◽  
High Space

Rational design LDHs (layered double hydroxides) with 3D hierarchical hollow structures have generated widespread interest for catalytic oxidation due to the high complexity in shell architecture and composition. Herein, we reported a handy two-step method to construct a 3D hierarchical NiCo2O4/NiO nanocage. This synthetic strategy contains a partial in situ transformation of ZIF-67 (zeolitic imidazolate framework-67) into Co-NiLDH yolk-shelled structures following ethanol etching, and a structure-preserved transformation from Co-NiLDH@ZIF-67 to a biphase nanocage following calcination. CoNi-yh-T (varied reaction time and calcination temperature) nanocages were investigated systematically by Brunauer–Emmett–Teller (BET), X-ray photoelectron spectroscopy (XPS), H2- temperature-programmed reduction (TPR), NH3-temperature-programmed desorption (TPD) and studied for toluene oxidation. The CoNi-6h-350 sample showed much higher activity with 90% toluene conversion (T90) at 229 °C at a high space velocity (SV = 60,000 mL g−1 h−1) than other catalysts (T90 >240 °C). Abundant surface high valence Co ions caused by the novel hierarchical nanostructures, together with adsorbed oxygen species and abundant medium-strength surface acid sites, played a key role for catalytic activities.

Rational design of a far-red fluorescent probe for endogenous biothiol imbalance induced by hydrogen peroxide in living cells and mice

2020 ◽  
Vol 103 ◽  
pp. 104173
Author(s):  
Zhengliang Lu ◽  
Xin Sun ◽  
Mengzhao Wang ◽  
Hongmei Wang ◽  
Chunhua Fan ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
Rational Design ◽  
Living Cells

Biomineralized Zeolitic Imidazolate Framework-8 Nanoparticles Enable Polymerase/Endonuclease Synergetic Amplification Reaction in Living Cells for Sensitive MicroRNA Imaging

2021 ◽  
Author(s):  
Ke Zhang ◽  
Xiayun Le ◽  
Qiaoqin Yu ◽  
Juan Zhang ◽  
Dandan Wang ◽  
...  
Keyword(s):  
Living Cells ◽  
Zeolitic Imidazolate Framework ◽  
Amplification Reaction

In this work, we have developed a simple-prepared nanocomplex probe, which could implement polymerase and endonuclease synergetic amplification reaction in living cells by the biomineralized ZIF-8 NPs for intracellular miRNA...

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