Nonblinking Carbon Dots for Imaging and Tracking Receptors on Live Cell Membrane

2021 ◽  
Author(s):  
Qian Wang ◽  
Zhenzhen Feng ◽  
Hua He ◽  
Xiang Hu ◽  
Jian Mao ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cell Membrane ◽  
Carbon Dots ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Semiconductor Quantum Dots ◽  
Live Cell

Blinking occurs with nearly all fluorophores including organic dyes, fluorescent proteins, semiconductor quantum dots and carbon dots (CDs). We developed non-blinking and photoresistant fluorescent CDs by introducing multiple aromatic domains...

Semiconductor Quantum Dots for Multicolor Fluorescence Imaging and Spectroscopy of Single Cancer Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
Xiaohu Gao ◽  
Shuming Nie ◽  
Wallace H. Coulter
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Semiconductor Quantum Dots ◽  
Single Cancer ◽  
Imaging And Spectroscopy

AbstractLuminescent quantum dots (QDs) are emerging as a new class of biological labels with unique properties and applications that are not available from traditional organic dyes and fluorescent proteins. Here we report new developments in using semiconductor quantum dots for quantitative imaging and spectroscopy of single cancer cells. We show that both live and fixed cells can be labeled with multicolor QDs, and that single cells can be analyzed by fluorescence imaging and wavelength-resolved spectroscopy. These results raise new possibilities in cancer imaging, molecular profiling, and disease staging.

scholarly journals Photoluminescent Nanomaterials for Medical Biotechnology

Acta Naturae ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 16-31
Author(s):  
Evgenii L. Guryev ◽  
Samah Shanwar ◽  
Andrei Vasilevich Zvyagin ◽  
Sergey M. Deyev ◽  
Irina V. Balalaeva
Keyword(s):  
Quantum Dots ◽  
Porous Silicon ◽  
Sensitivity And Specificity ◽  
Carbon Dots ◽  
Photophysical Properties ◽  
Gold Nanoclusters ◽  
Semiconductor Quantum Dots ◽  
Diagnostic Methods ◽  
Optical Diagnostic ◽  
Therapy Effectiveness

Creation of various photoluminescent nanomaterials has significantly expanded the arsenal of approaches used in modern biomedicine. Their unique photophysical properties can significantly improve the sensitivity and specificity of diagnostic methods, increase therapy effectiveness, and make a theranostic approach to treatment possible through the application of nanoparticle conjugates with functional macromolecules. The most widely used nanomaterials to date are semiconductor quantum dots; gold nanoclusters; carbon dots; nanodiamonds; semiconductor porous silicon; and up-conversion nanoparticles. This paper considers the promising groups of photoluminescent nanomaterials that can be used in medical biotechnology: in particular, for devising agents for optical diagnostic methods, sensorics, and various types of therapy.

scholarly journals Bioluminescence-Based Energy Transfer Using Semiconductor Quantum Dots as Acceptors

Sensors ◽  
2020 ◽  
Vol 20 (10) ◽  
pp. 2909 ◽  
Author(s):  
Anirban Samanta ◽  
Igor L. Medintz
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Organic Dyes ◽  
Stokes Shift ◽  
Resonance Energy Transfer ◽  
Fluorescence Emission ◽  
Resonance Energy ◽  
Semiconductor Quantum Dots ◽  
Renilla Luciferase ◽  
Bioluminescent Protein

Bioluminescence resonance energy transfer (BRET) is the non-radiative transfer of energy from a bioluminescent protein donor to a fluorophore acceptor. It shares all the formalism of Förster resonance energy transfer (FRET) but differs in one key aspect: that the excited donor here is produced by biochemical means and not by an external illumination. Often the choice of BRET source is the bioluminescent protein Renilla luciferase, which catalyzes the oxidation of a substrate, typically coelenterazine, producing an oxidized product in its electronic excited state that, in turn, couples with a proximal fluorophore resulting in a fluorescence emission from the acceptor. The acceptors pertinent to this discussion are semiconductor quantum dots (QDs), which offer some unrivalled photophysical properties. Amongst other advantages, the QD’s large Stokes shift is particularly advantageous as it allows easy and accurate deconstruction of acceptor signal, which is difficult to attain using organic dyes or fluorescent proteins. QD-BRET systems are gaining popularity in non-invasive bioimaging and as probes for biosensing as they don’t require external optical illumination, which dramatically improves the signal-to-noise ratio by avoiding background auto-fluorescence. Despite the additional advantages such systems offer, there are challenges lying ahead that need to be addressed before they are utilized for translational types of research.

Construction and Application of Semiconductor Quantum Dots and Carbon Dots Based 0D/2D Heterojunction Photocatalysts

2021 ◽  
Vol 42 (08) ◽  
pp. 1278-1296
Author(s):  
Dong-qi ZHANG ◽  
◽  
Jun-peng NI ◽  
Qi-tao CHEN ◽  
Yan-hong LIU ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Carbon Dots ◽  
Semiconductor Quantum Dots

scholarly journals Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

2017 ◽  
Vol 8 ◽  
pp. 1238-1249 ◽  
Author(s):  
Jennifer E Francis ◽  
David Mason ◽  
Raphaël Lévy
Keyword(s):  
Quantum Dots ◽  
Fluorescent Dyes ◽  
Fluorescent Proteins ◽  
Semiconductor Quantum Dots ◽  
Secondary Antibody ◽  
Igg Antibodies ◽  
Protein Targets ◽  
Cellular Targets ◽  
Extensive Cell ◽  
Specific Labelling

Semiconductor quantum dots (Qdots) have been utilised as probes in fluorescence microscopy and provide an alternative to fluorescent dyes and fluorescent proteins due to their brightness, photostability, and the possibility to excite different Qdots with a single wavelength. In spite of these attractive properties, their implemenation by biologists has been somewhat limited and only a few Qdot conjugates are commercially available for the labelling of cellular targets. Although many protocols have been reported for the specific labelling of proteins with Qdots, the majority of these relied on Qdot-conjugated antibodies synthesised specifically by the authors (and therefore not widely available), which limits the scope of applications and complicates replication. Here, the specificity of a commercially available, Qdot-conjugated secondary antibody (Qdot-Ab) was tested against several primary IgG antibodies. The antigens were labelled simultaneously with a fluorescent dye coupled to a secondary antibody (Dye-Ab) and the Qdot-Ab. Although, the Dye-Ab labelled all of the intended target proteins, the Qdot-Ab was found bound to only some of the protein targets in the cytosol and could not reach the nucleus, even after extensive cell permeabilisation.

scholarly journals Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

2016 ◽  
Author(s):  
Jennifer E. Francis ◽  
David Mason ◽  
Raphaël Lévy
Keyword(s):  
Quantum Dots ◽  
Fluorescent Dyes ◽  
Fluorescent Proteins ◽  
Fluorescent Microscopy ◽  
Semiconductor Quantum Dots ◽  
Secondary Antibody ◽  
Protein Targets ◽  
Cellular Targets ◽  
Extensive Cell ◽  
Specific Labelling

AbstractSemiconductor quantum dots (Qdots) have been utilised as probes in fluorescent microscopy and provide an alternative to fluorescent dyes and fluorescent proteins, due to their brightness, photostability, and the possibility to excite different Qdots with a single wavelength. In spite of these attractive properties, their take up by biologists has been somewhat limited and only a few Qdot conjugates are commercially available for the labelling of cellular targets. Although, many protocols have been reported for the specific labelling of proteins with Qdots, the majority of these relied on Qdot-conjugated antibodies synthesised specifically by the authors and therefore not broadly available, which limits the scope of applications and complicates replication. Here, the specificity of a commercially available Qdot conjugated secondary antibody (Qdot-Ab), for different antigens, was tested. Antigens were labelled simultaneously with a fluorescent dye coupled to a secondary antibody (Dye-Ab) and the Qdot-Ab. Although, the Dye-Ab labelled all of the intended target proteins, the Qdot-Ab only bound to some of the protein targets in the cytosol and could not reach the nucleus even after extensive cell permeabilisation.

Quantum Dots and FRET-Nanobeads for Probing Genes, Proteins, and Drug Targets in Single Cells

2003 ◽  
Author(s):  
Amit Agrawal ◽  
Xiaohu Gao ◽  
Nitin Nitin ◽  
Gang Bao ◽  
Shuming Nie
Keyword(s):  
Quantum Dots ◽  
Drug Targets ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Length Scale ◽  
Imaging And Spectroscopy

Quantum dots are tiny light-emitting particles on the length scale of 2–10 nm, and FRET-nanobeads for fluorophore-embedded nanoparticles on the length scale of 40–200 nm based on the phenomenon of fluorescence resonance energy transfer (FRET). These materials are emerging as a new class of biological labels with properties and applications that are not available with traditional organic dyes and fluorescent proteins. In this ASME contribution, we report new developments in using semiconductor quantum dots for quantitative imaging and spectroscopy of single cancer cells. We also show results from intracellular staining of actin filaments using FRET-nanobeads. These results raise new possibilities in disease diagnostics, drug and biochemical discovery, cancer imaging, molecular profiling, and disease staging.

Sign in / Sign up

Export Citation Format

Share Document