Application of nanoparticles in bone tissue engineering; a review on the molecular mechanisms driving osteogenesis

2021 ◽  
Author(s):  
Azam Bozorgi ◽  
Mozafar Khazaei ◽  
Mansoureh Soleimani ◽  
Zahra Jamalpoor
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Fate ◽  
Molecular Mechanisms ◽  
Bone Defects ◽  
Large Bone ◽  
Large Bone Defects

The introduction of nanoparticles into bone tissue engineering strategies is beneficial to govern cell fate into osteogenesis and the regeneration of large bone defects. The present study explored the role...

Study of the Effect of Mechanical Loading on Cell Cultures in Bone Tissue Engineering

2012 ◽  
Author(s):  
Magali Cruel ◽  
Morad Bensidhoum ◽  
Laure Sudre ◽  
Guillaume Puel ◽  
Virginie Dumas ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Defects ◽  
Improved Design ◽  
Large Bone ◽  
Large Bone Defects

Bone tissue engineering currently represents one of the most interesting alternatives to autologous transplants and their drawbacks in the treatment of large bone defects. Mesenchymal stem cells are used to build new bone in vitro in a bioreactor. Their stimulation and our understanding of the mechanisms of mechanotransduction need to be improved in order to optimize the design of bioreactors. In this study, several geometries of bioreactor were analyzed experimentally and biological results were linked with numerical simulations of the flow inside the bioreactor. These results will constitute a base for an improved design of the existing bioreactor.

Biomimetic hydroxyapatite-containing composite nanofibrous substrates for bone tissue engineering

2010 ◽  
Vol 368 (1917) ◽  
pp. 2065-2081 ◽  
Author(s):  
J. Venugopal ◽  
Molamma P. Prabhakaran ◽  
Yanzhong Zhang ◽  
Sharon Low ◽  
Aw Tar Choon ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Natural Polymer ◽  
Natural Polymers ◽  
Biomimetic Hydroxyapatite ◽  
Large Bone ◽  
Large Bone Defects

The fracture of bones and large bone defects owing to various traumas or natural ageing is a typical type of tissue malfunction. Surgical treatment frequently requires implantation of a temporary or permanent prosthesis, which is still a challenge for orthopaedic surgeons, especially in the case of large bone defects. Mimicking nanotopography of natural extracellular matrix (ECM) is advantageous for the successful regeneration of damaged tissues or organs. Electrospun nanofibre-based synthetic and natural polymer scaffolds are being explored as a scaffold similar to natural ECM for tissue engineering applications. Nanostructured materials are smaller in size falling, in the 1–100 nm range, and have specific properties and functions related to the size of the natural materials (e.g. hydroxyapatite (HA)). The development of nanofibres with nano-HA has enhanced the scope of fabricating scaffolds to mimic the architecture of natural bone tissue. Nanofibrous substrates supporting adhesion, proliferation, differentiation of cells and HA induce the cells to secrete ECM for mineralization to form bone in bone tissue engineering. Our laboratory (NUSNNI, NUS) has been fabricating a variety of synthetic and natural polymer-based nanofibrous substrates and synthesizing HA for blending and spraying on nanofibres for generating artificial ECM for bone tissue regeneration. The present review is intended to direct the reader’s attention to the important subjects of synthetic and natural polymers with HA for bone tissue engineering.

scholarly journals Clinical Applications of Cell-Scaffold Constructs for Bone Regeneration Therapy

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2687
Author(s):  
Venkata Suresh Venkataiah ◽  
Yoshio Yahata ◽  
Akira Kitagawa ◽  
Masahiko Inagaki ◽  
Yusuke Kakiuchi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Defects ◽  
Significant Loss ◽  
Osteogenic Potential ◽  
Large Bone ◽  
Large Bone Defects

Bone tissue engineering (BTE) is a process of combining live osteoblast progenitors with a biocompatible scaffold to produce a biological substitute that can integrate into host bone tissue and recover its function. Mesenchymal stem cells (MSCs) are the most researched post-natal stem cells because they have self-renewal properties and a multi-differentiation capacity that can give rise to various cell lineages, including osteoblasts. BTE technology utilizes a combination of MSCs and biodegradable scaffold material, which provides a suitable environment for functional bone recovery and has been developed as a therapeutic approach to bone regeneration. Although prior clinical trials of BTE approaches have shown promising results, the regeneration of large bone defects is still an unmet medical need in patients that have suffered a significant loss of bone function. In this present review, we discuss the osteogenic potential of MSCs in bone tissue engineering and propose the use of immature osteoblasts, which can differentiate into osteoblasts upon transplantation, as an alternative cell source for regeneration in large bone defects.

scholarly journals The Potential of FGF-2 in Craniofacial Bone Tissue Engineering: A Review

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 932
Author(s):  
Anita Novais ◽  
Eirini Chatzopoulou ◽  
Catherine Chaussain ◽  
Caroline Gorin
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Current Knowledge ◽  
The Body ◽  
Bone Homeostasis ◽  
Craniofacial Bone ◽  
Bone Damage ◽  
Large Bone ◽  
Large Bone Defects

Bone is a hard-vascularized tissue, which renews itself continuously to adapt to the mechanical and metabolic demands of the body. The craniofacial area is prone to trauma and pathologies that often result in large bone damage, these leading to both aesthetic and functional complications for patients. The “gold standard” for treating these large defects is autologous bone grafting, which has some drawbacks including the requirement for a second surgical site with quantity of bone limitations, pain and other surgical complications. Indeed, tissue engineering combining a biomaterial with the appropriate cells and molecules of interest would allow a new therapeutic approach to treat large bone defects while avoiding complications associated with a second surgical site. This review first outlines the current knowledge of bone remodeling and the different signaling pathways involved seeking to improve our understanding of the roles of each to be able to stimulate or inhibit them. Secondly, it highlights the interesting characteristics of one growth factor in particular, FGF-2, and its role in bone homeostasis, before then analyzing its potential usefulness in craniofacial bone tissue engineering because of its proliferative, pro-angiogenic and pro-osteogenic effects depending on its spatial-temporal use, dose and mode of administration.

Strontium-calcium phosphate hybrid cement with enhanced osteogenic and angiogenic properties for vascularised bone regeneration

2021 ◽  
Author(s):  
Xiexing Wu ◽  
Ziniu Tang ◽  
Kang Wu ◽  
Yanjie Bai ◽  
X. LIN ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Calcium Phosphate Cement ◽  
Treatment Options ◽  
Bone Defects ◽  
Large Bone ◽  
Large Bone Defects ◽  
Vascularized Bone

Vascularized bone tissue engineering is regarded as one of the optimal treatment options for large bone defects. The lack of angiogenic property and unsatisfactory physicochemical performance restricts calcium phosphate cement...

scholarly journals Dual Network Composites of Poly(vinyl alcohol)-Calcium Metaphosphate/Alginate with Osteogenic Ions for Bone Tissue Engineering in Oral and Maxillofacial Surgery

2021 ◽  
Vol 8 (8) ◽  
pp. 107
Author(s):  
Lilis Iskandar ◽  
Lucy DiSilvio ◽  
Jonathan Acheson ◽  
Sanjukta Deb
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Vinyl Alcohol ◽  
Maxillofacial Surgery ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Poly Vinyl Alcohol ◽  
Oral And Maxillofacial Surgery

Despite considerable advances in biomaterials-based bone tissue engineering technologies, autografts remain the gold standard for rehabilitating critical-sized bone defects in the oral and maxillofacial (OMF) region. A majority of advanced synthetic bone substitutes (SBS’s) have not transcended the pre-clinical stage due to inferior clinical performance and translational barriers, which include low scalability, high cost, regulatory restrictions, limited advanced facilities and human resources. The aim of this study is to develop clinically viable alternatives to address the challenges of bone tissue regeneration in the OMF region by developing ‘dual network composites’ (DNC’s) of calcium metaphosphate (CMP)—poly(vinyl alcohol) (PVA)/alginate with osteogenic ions: calcium, zinc and strontium. To fabricate DNC’s, single network composites of PVA/CMP with 10% (w/v) gelatine particles as porogen were developed using two freeze–thawing cycles and subsequently interpenetrated by guluronate-dominant sodium alginate and chelated with calcium, zinc or strontium ions. Physicochemical, compressive, water uptake, thermal, morphological and in vitro biological properties of DNC’s were characterised. The results demonstrated elastic 3D porous scaffolds resembling a ‘spongy bone’ with fluid absorbing capacity, easily sculptable to fit anatomically complex bone defects, biocompatible and osteoconductive in vitro, thus yielding potentially clinically viable for SBS alternatives in OMF surgery.

Fully Interconnected Globular Porous Biphasic Calcium Phosphate Ceramic Scaffold Facilitates Osteogenic Repair

2007 ◽  
Vol 361-363 ◽  
pp. 119-122 ◽  
Author(s):  
J.H. Lim ◽  
J.H. Park ◽  
Eui Kyun Park ◽  
Hae Jung Kim ◽  
Il Kyu Park ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Substitute ◽  
Biphasic Calcium Phosphate ◽  
Bone Defects ◽  
Calcium Phosphate Ceramic ◽  
Ceramic Scaffold ◽  
Porous Biphasic Calcium Phosphate

An appropriate scaffold, which provides structural support for transplanted cells and acts as a vehicle for the delivery of biologically active molecules, is critical for tissue engineering. We developed a fully interconnected globular porous biphasic calcium phosphate ceramic scaffold by adopting a foaming method, and evaluated its efficiency as a bone substitute and a scaffold for bone tissue engineering by in vitro and in vivo biocompatible analysis and its osteogenic healing capacity in rat tibial bone defects. They have spherical pores averaging 400um in diameter and interconnecting interpores averaging 70um in diameter with average 85% porosity. They elicited no cytotoxicity and noxious effect on cellular proliferation and osteoblastic differentiation during the cell-scaffold construct formation. Also the bone defects grafted with fully interconnected globular porous biphasic calcium phosphate ceramic blocks revealed excellent bone healing within 3 weeks. These findings suggest that the fully interconnected porous biphasic calcium phosphate scaffold formed by the foaming method can be a promising bone substitute and a scaffold for bone tissue engineering.

Comparison among Four Kinds of Bone Tissue Engineering Scaffold

2007 ◽  
Vol 330-332 ◽  
pp. 963-966 ◽  
Author(s):  
Lei Liu ◽  
Run Liang Chen ◽  
Yun Feng Lin ◽  
Cai Li ◽  
Wei Dong Tian ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Bone Defects ◽  
Marrow Stromal Cells ◽  
Autogenous Bone ◽  
Calvarial Defects ◽  
Reconstruction Of Bone Defects

Bone tissue engineering is a promising way to repair of bone defects. To choose a proper scaffold is still a disputable problem in bone tissue engineering. This study aimed to compare the effects of repairing critical calvarial defects with the compounds of autogenous bone marrow stromal cells (BMSCs) and coral hydroxyapatite(CHA), hydroxyapatite/ tricalcium phosphate (HA/TCP), poly(lactide-co-glycolide) (PLGA) and alginate (AG). The results showed that CHA and AG were satisfactory bone tissues engineering scaffolds among the four kinds of materials. BMSCs/CHA and BMSCs/AG are promising techniques for reconstruction of bone defects.

Preparation and characterization of calcium phosphate/pectin scaffolds for bone tissue engineering

RSC Advances ◽  
2016 ◽  
Vol 6 (67) ◽  
pp. 62071-62082 ◽  
Author(s):  
Lisheng Zhao ◽  
Junjie Li ◽  
Liang Zhang ◽  
Yu Wang ◽  
Jiexin Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Compressive Strength ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Clinical Application ◽  
Calcium Phosphate Cement ◽  
Bone Defects

A calcium phosphate cement (CPC) scaffold has been used to repair bone defects, but its low compressive strength and poor osteogenesis greatly hinder its clinical application.

Reconstruction of Cranial Bone Defects Using a Compound of Bone Marrow Stromal Cells and Hydroxyapatite-Tricalcium Phosphate

2008 ◽  
Vol 368-372 ◽  
pp. 1235-1237 ◽  
Author(s):  
Lei Liu ◽  
Run Liang Chen ◽  
Yun Feng Lin ◽  
Wei Dong Tian ◽  
Sheng Wei Li
Keyword(s):  
Electron Microscopy ◽  
Tissue Engineering ◽  
Bone Marrow ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Stromal Cells ◽  
Tricalcium Phosphate ◽  
Bone Marrow Stromal Cells ◽  
Bone Defects ◽  
Marrow Stromal Cells

Hydroxyapatite-tricalcium phosphate (HA-TCP) is a new kind of material which shows good biocompatibility, biological degradability, and porosity. This study aimed to determine the effectiveness of HA-TCP as a bone tissue engineering scaffold. In this study, critical size cranial defects were reconstructed with compounds of autogenous bone marrow stromal cells (BMSCs) and HA-TCP. The resulting grafts were examined by X-ray, histological examination, semi-quantitative analysis of osteogenesis, immunochemical examination (collagen type I and III), scanning electron microscopy and transmission electron microscopy. The results showed that HA-TCP is a good bone tissue engineering scaffold and BMSCs/HA-TCP is a promising technique for reconstruction of bone defects.

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