Rapid, instrument-free colorimetric quantification of DNA using Nile Blue

2022 ◽  
Author(s):  
Heather D. Whitehead ◽  
Marya Lieberman
Keyword(s):  
Nucleic Acid ◽  
Human Health ◽  
Rapid Detection ◽  
Nile Blue ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Techniques ◽  
Nucleic Acid Tests

The use of nucleic acid tests (NAT) for sensitive and rapid detection of pathogens relevant to human health has increased due to the ubiquity of nucleic acid amplification techniques such...

scholarly journals Automated multiplex nucleic acid tests for rapid detection of SARS-CoV-2, influenza A and B infection with direct reverse-transcription quantitative PCR (dirRT-qPCR) assay in a centrifugal microfluidic platform

RSC Advances ◽  
2020 ◽  
Vol 10 (56) ◽  
pp. 34088-34098
Author(s):  
Minghui Ji ◽  
Yun Xia ◽  
Jacky Fong-Chuen Loo ◽  
Lang Li ◽  
Ho-Pui Ho ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Quantitative Pcr ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Influenza A ◽  
Qpcr Assay ◽  
Multiplex Detection ◽  
Microfluidic Platform ◽  
Reverse Transcription Quantitative Pcr ◽  
Nucleic Acid Tests

Development of a microfluidic disc-direct reverse-transcription quantitative PCR platform to perform automated multiplex nucleic acid tests for rapid multiplex detection of disease infection.

A collaborative study to establish the first National Standard for HIV-1 RNA nucleic acid amplification techniques (NAT) in Taiwan

2013 ◽  
Vol 191 (2) ◽  
pp. 122-127
Author(s):  
Yi-Chen Yang ◽  
Daniel Yang-Chih Shih ◽  
Mong-Hsun Tsai ◽  
Chia-Hung Cheng ◽  
Hwei-Fang Cheng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Collaborative Study ◽  
Nucleic Acid Amplification ◽  
National Standard ◽  
Nucleic Acid Amplification Techniques ◽  
Hiv 1

Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory.

1997 ◽  
Vol 10 (2) ◽  
pp. 242-256 ◽  
Author(s):  
M Ieven ◽  
H Goossens
Keyword(s):  
Nucleic Acid ◽  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Clinical Laboratory ◽  
Pneumocystis Carinii ◽  
Diagnostic Techniques ◽  
Nucleic Acid Amplification ◽  
Molecular Diagnostic ◽  
Nucleic Acid Amplification Techniques ◽  
Tract Infections

Clinical laboratories are increasingly receiving requests to perform nucleic acid amplification tests for the detection of a wide variety of infectious agents. In this paper, the efficiency of nucleic acid amplification techniques for the diagnosis of respiratory tract infections is reviewed. In general, these techniques should be applied only for the detection of microorganisms for which available diagnostic techniques are markedly insensitive or nonexistent or when turnaround times for existing tests (e.g., viral culture) are much longer than those expected with amplification. This is the case for rhinoviruses, coronaviruses, and hantaviruses causing a pulmonary syndrome, Bordetella pertussis, Chlamydia pneumoniae, Mycoplasma pneumoniae, and Coxiella burnetii. For Legionella spp. and fungi, contamination originating from the environment is a limiting factor in interpretation of results, as is the difficulty in differentiating colonization and infection. Detection of these agents in urine or blood by amplification techniques remains to be evaluated. In the clinical setting, there is no need for molecular diagnostic tests for the diagnosis of Pneumocystis carinii. At present, amplification methods for Mycobacterium tuberculosis cannot replace the classical diagnostic techniques, due to their lack of sensitivity and the absence of specific internal controls for the detection of inhibitors of the reaction. Also, the results of interlaboratory comparisons are unsatisfactory. Furthermore, isolates are needed for susceptibility studies. Additional work remains to be done on sample preparation methods, comparison between different amplification methods, and analysis of results. The techniques can be useful for the rapid identification of M. tuberculosis in particular circumstances, as well as the rapid detection of most rifampin-resistant isolates. The introduction of diagnostic amplification techniques into a clinical laboratory implies a level of proficiency for excluding false-positive and false-negative results.

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