scholarly journals Mycoplasma pneumoniae: Current Knowledge on Nucleic Acid Amplification Techniques and Serological Diagnostics

2016 ◽  
Vol 7 ◽  
Author(s):  
Katherine Loens ◽  
Margareta Ieven
Keyword(s):  
Nucleic Acid ◽  
Mycoplasma Pneumoniae ◽  
Current Knowledge ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Techniques ◽  
Serological Diagnostics

A collaborative study to establish the first National Standard for HIV-1 RNA nucleic acid amplification techniques (NAT) in Taiwan

2013 ◽  
Vol 191 (2) ◽  
pp. 122-127
Author(s):  
Yi-Chen Yang ◽  
Daniel Yang-Chih Shih ◽  
Mong-Hsun Tsai ◽  
Chia-Hung Cheng ◽  
Hwei-Fang Cheng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Collaborative Study ◽  
Nucleic Acid Amplification ◽  
National Standard ◽  
Nucleic Acid Amplification Techniques ◽  
Hiv 1

Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory.

1997 ◽  
Vol 10 (2) ◽  
pp. 242-256 ◽  
Author(s):  
M Ieven ◽  
H Goossens
Keyword(s):  
Nucleic Acid ◽  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Clinical Laboratory ◽  
Pneumocystis Carinii ◽  
Diagnostic Techniques ◽  
Nucleic Acid Amplification ◽  
Molecular Diagnostic ◽  
Nucleic Acid Amplification Techniques ◽  
Tract Infections

Clinical laboratories are increasingly receiving requests to perform nucleic acid amplification tests for the detection of a wide variety of infectious agents. In this paper, the efficiency of nucleic acid amplification techniques for the diagnosis of respiratory tract infections is reviewed. In general, these techniques should be applied only for the detection of microorganisms for which available diagnostic techniques are markedly insensitive or nonexistent or when turnaround times for existing tests (e.g., viral culture) are much longer than those expected with amplification. This is the case for rhinoviruses, coronaviruses, and hantaviruses causing a pulmonary syndrome, Bordetella pertussis, Chlamydia pneumoniae, Mycoplasma pneumoniae, and Coxiella burnetii. For Legionella spp. and fungi, contamination originating from the environment is a limiting factor in interpretation of results, as is the difficulty in differentiating colonization and infection. Detection of these agents in urine or blood by amplification techniques remains to be evaluated. In the clinical setting, there is no need for molecular diagnostic tests for the diagnosis of Pneumocystis carinii. At present, amplification methods for Mycobacterium tuberculosis cannot replace the classical diagnostic techniques, due to their lack of sensitivity and the absence of specific internal controls for the detection of inhibitors of the reaction. Also, the results of interlaboratory comparisons are unsatisfactory. Furthermore, isolates are needed for susceptibility studies. Additional work remains to be done on sample preparation methods, comparison between different amplification methods, and analysis of results. The techniques can be useful for the rapid identification of M. tuberculosis in particular circumstances, as well as the rapid detection of most rifampin-resistant isolates. The introduction of diagnostic amplification techniques into a clinical laboratory implies a level of proficiency for excluding false-positive and false-negative results.

Nucleic acid amplification techniques (NAATs) for early diagnosis of HIV-1 and HIV-2 infections

2015 ◽  
Author(s):  
Regina El Dib ◽  
Mariska MG Leeflang ◽  
Joseph L Mathew ◽  
Ricardo AMB Almeida ◽  
David S Lewi ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Early Diagnosis ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Techniques ◽  
Hiv 1
Sign in / Sign up

Export Citation Format

Share Document