Graphene Oxide/Perylene-Aniline Electrochemiluminescence Platform for Protein Detection Based on Molecule Recognition

2021 ◽  
Author(s):  
Peng Yin ◽  
Wei Zhang ◽  
Lei Shang ◽  
Rong-Na Ma ◽  
Liping Jia ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Folic Acid ◽  
Small Molecule ◽  
Folate Receptor ◽  
Protein Detection ◽  
Single Strand ◽  
Molecule Recognition

Most biosensors for protein folate receptor(FR) detection based on small molecule folic acid(FA) recognition usually introduced FA linked single strand DNA(FA-ssDNA) and nuclease to promote sensitivity, which increased expenses and...

scholarly journals A DNA Electrochemical Sensor via Terminal Protection of Small-Molecule-Linked DNA for Highly Sensitive Protein Detection

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 451
Author(s):  
Ping Ouyang ◽  
Chenxin Fang ◽  
Jialun Han ◽  
Jingjing Zhang ◽  
Yuxing Yang ◽  
...  
Keyword(s):  
Small Molecule ◽  
Gold Electrode ◽  
Folate Receptor ◽  
Protein Detection ◽  
Disease Diagnosis ◽  
Dna Biosensor ◽  
Target Protein ◽  
Receptor Protein ◽  
Electrochemical Dna Biosensor ◽  
Electrochemical Signal

The qualitative and quantitative determination of marker protein is of great significance in the life sciences and in medicine. Here, we developed an electrochemical DNA biosensor for protein detection based on DNA self-assembly and the terminal protecting effects of small-molecule-linked DNA. This strategy is demonstrated using the small molecule biotin and its receptor protein streptavidin (SA). We immobilized DNA with a designed structure and sequence on the surface of the gold electrode, and we named it M1-Biotin DNA. M1-Biotin DNA selectively combines with SA to generate M1-Biotin-SA DNA and protects M1-Biotin DNA from digestion by EXO III; therefore, M1-Biotin DNA remains intact on the electrode surface. M1-Biotin-SA DNA was modified with methylene blue (MB); the MB reporter molecule is located near the surface of the gold electrode, which generates a substantial electrochemical signal during the detection of SA. Through this strategy, we can exploit the presence or absence of an electrochemical signal to provide qualitative target protein determination as well as the strength of the electrochemical signal to quantitatively analyze the target protein concentration. This strategy has been proven to be used for the quantitative analysis of the interaction between biotin and streptavidin (SA). Under optimal conditions, the detection limit of the proposed biosensor is as low as 18.8 pM, and the linear range is from 0.5 nM to 5 μM, showing high sensitivity. The detection ability of this DNA biosensor in complex serum samples has also been studied. At the same time, we detected the folate receptor (FR) to confirm that this strategy can be used to detect other proteins. Therefore, this electrochemical DNA biosensor provides a sensitive, low-cost, and fast target protein detection platform, which may provide a reliable and powerful tool for early disease diagnosis.

scholarly journals Folate receptor targeted delivery of paclitaxel to breast cancer cells via folic acid conjugated graphene oxide grafted methyl acrylate nanocarrier

2019 ◽  
Vol 110 ◽  
pp. 906-917 ◽  
Author(s):  
Kandasamy Vinothini ◽  
Naresh Kumar Rajendran ◽  
Andy Ramu ◽  
Nandhakumar Elumalai ◽  
Mariappan Rajan
Keyword(s):  
Breast Cancer ◽  
Graphene Oxide ◽  
Folic Acid ◽  
Cancer Cells ◽  
Methyl Acrylate ◽  
Targeted Delivery ◽  
Breast Cancer Cells ◽  
Folate Receptor

Graphene Oxide-Based Fluorescent Biosensor for Protein Detection via Terminal Protection of Small-Molecule-Linked DNA

Small ◽  
2013 ◽  
Vol 9 (12) ◽  
pp. 2097-2101 ◽  
Author(s):  
Yue He ◽  
Xiaojing Xing ◽  
Hongwu Tang ◽  
Daiwen Pang
Keyword(s):  
Graphene Oxide ◽  
Small Molecule ◽  
Protein Detection ◽  
Fluorescent Biosensor

A ratiometric colorimetric detection of the folate receptor based on terminal protection of small-molecule-linked DNA

The Analyst ◽  
2015 ◽  
Vol 140 (4) ◽  
pp. 1260-1264 ◽  
Author(s):  
Yanhong Zhu ◽  
Guangfeng Wang ◽  
Liang Sha ◽  
Yuwei Qiu ◽  
Hong Jiang ◽  
...  
Keyword(s):  
Small Molecule ◽  
Crucial Role ◽  
Folate Receptor ◽  
Low Cost ◽  
Colorimetric Detection ◽  
Cancer Therapeutics ◽  
Selective Detection

Development of strategies for the sensitive and selective detection of the folate receptor (FR) that are simple and low cost is of great importance for assessing cancer therapeutics due to its crucial role in physiological, pharmacological and pathological processes.

Folic acid-conjugated green luminescent carbon dots as a nanoprobe for identifying folate receptor-positive cancer cells

Talanta ◽  
2018 ◽  
Vol 183 ◽  
pp. 39-47 ◽  
Author(s):  
Junli Zhang ◽  
Xuewei Zhao ◽  
Ming Xian ◽  
Chuan Dong ◽  
Shaomin Shuang
Keyword(s):  
Folic Acid ◽  
Cancer Cells ◽  
Carbon Dots ◽  
Folate Receptor

scholarly journals Interplay of cysteine exposure and global protein dynamics in small-molecule recognition by a regulator of G-protein signaling protein

2018 ◽  
Vol 87 (2) ◽  
pp. 146-156 ◽  
Author(s):  
Mohammadjavad Mohammadi ◽  
Hossein Mohammadiarani ◽  
Vincent S. Shaw ◽  
Richard R. Neubig ◽  
Harish Vashisth
Keyword(s):  
Protein Dynamics ◽  
G Protein ◽  
Small Molecule ◽  
G Protein Signaling ◽  
Protein Signaling ◽  
Signaling Protein ◽  
Molecule Recognition

scholarly journals Phosphorylcholine oligomer-grafted graphene oxide for tumor-targeting doxorubicin delivery

RSC Advances ◽  
2017 ◽  
Vol 7 (66) ◽  
pp. 41675-41685 ◽  
Author(s):  
Yu Qin ◽  
Changyu Wang ◽  
Yun Jiang ◽  
Tao Liu ◽  
Jianyong Yang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Graphene Oxide ◽  
Folic Acid ◽  
Tumor Targeting ◽  
Doxorubicin Delivery

Phosphorylcholine oligomer-grafted, folic acid-labeled and doxorubicin-loaded graphene oxide was found to be a potential versatile platform for biomarker-directed drug delivery with optimal biocompatibility.

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