Smartphone Digital Image Colorimetric Determination of the Total Monomeric Anthocyanin Content in Black Rice via the pH Differential Method

2021 ◽  
Author(s):  
Chonlada Bennett ◽  
Phumon Sookwong ◽  
Jaroon Jakmunee ◽  
Sugunya Mahatheeranont
Keyword(s):  
Mobile Phone ◽  
Digital Image ◽  
Differential Method ◽  
Anthocyanin Content ◽  
Colorimetric Determination ◽  
Black Rice ◽  
System P ◽  
First Time

In this research, the pH differential method was explored for the first time using a mobile phone as a detector, replacing UV-Vis spectrophotometry. A smartphone digital image colorimetric (SDIC) system...

Sphere-like CoS with nanostructures as peroxidase mimics for colorimetric determination of H2O2 and mercury ions

RSC Advances ◽  
2016 ◽  
Vol 6 (71) ◽  
pp. 66963-66970 ◽  
Author(s):  
Haiguan Yang ◽  
Junqi Zha ◽  
Peng Zhang ◽  
Yuhao Xiong ◽  
Linjing Su ◽  
...  
Keyword(s):  
Solvothermal Method ◽  
Analytical Techniques ◽  
Colorimetric Determination ◽  
Mercury Ions ◽  
Peroxidase Mimics ◽  
First Time ◽  
Facile Solvothermal Method

CoS, which was prepared using a facile solvothermal method, and characterized using various analytical techniques, was demonstrated for the first time to exhibit intrinsic peroxidase-like activity.

Convenient and sensitive colorimetric determination of alendronate sodium with Ce4+-triggered oxidation of TMB

2020 ◽  
Vol 44 (30) ◽  
pp. 12962-12966
Author(s):  
Jing Sun ◽  
Rui Wang ◽  
Meng Xia ◽  
Shuyun Zhu ◽  
Xian-En Zhao
Keyword(s):  
Colorimetric Assay ◽  
Colorimetric Determination ◽  
Selective Detection ◽  
Alendronate Sodium ◽  
First Time

A facile colorimetric assay for the sensitive and selective detection of alendronate sodium has been developed based on Ce4+-triggered oxidation of TMB for the first time.

Colorimetric determination of thiol compounds in serum based on Fe-MIL-88NH2 metal–organic framework as peroxidase mimetics

2014 ◽  
Vol 6 (15) ◽  
pp. 5647-5651 ◽  
Author(s):  
Zhongwei Jiang ◽  
Yali Liu ◽  
Xiaoli Hu ◽  
Yuanfang Li
Keyword(s):  
Catalytic Oxidation ◽  
Metal Organic Framework ◽  
New Method ◽  
Colorimetric Determination ◽  
Competitive Reaction ◽  
Thiol Compounds ◽  
System P ◽  
Metal Organic ◽  
Oxidation System

A new method for rapid and sensitive detection of biothiols has been developed based on the competitive reaction between TMB and thiol compounds with ˙OH in Fe-MIL-88NH2/H2O2 catalytic oxidation system.

scholarly journals New insight on the simultaneous H2 and HNO2 production in concentrated HNO3 aqueous solutions under alpha radiation

RSC Advances ◽  
2021 ◽  
Vol 11 (20) ◽  
pp. 12141-12152
Author(s):  
Raluca M. Musat ◽  
Jean-Luc Roujou ◽  
Vincent Dauvois ◽  
Muriel Ferry ◽  
Carole Marchand ◽  
...  
Keyword(s):  
Aqueous Solutions ◽  
Precise Determination ◽  
Alpha Radiation ◽  
System P ◽  
Set Up ◽  
First Time

For the first time ever, a specially designed set-up, coupled to the CEMHTI cyclotron allowed in situ monitoring of HNO2 and precise determination of H2 production in the external α radiolysis of HNO3 solution, while ensuring no perturbation of the investigated system.

scholarly journals Developing a novel paper-based enzymatic biosensor assisted by digital image processing and first-order multivariate calibration for rapid determination of nitrate in food samples

RSC Advances ◽  
2018 ◽  
Vol 8 (41) ◽  
pp. 23411-23420 ◽  
Author(s):  
Ali R. Jalalvand ◽  
Majid Mahmoudi ◽  
Hector C. Goicoechea
Keyword(s):  
Image Processing ◽  
Digital Image Processing ◽  
Digital Image ◽  
Multivariate Calibration ◽  
Rapid Determination ◽  
Food Samples ◽  
First Order ◽  
Enzymatic Biosensor ◽  
First Time

For the first time, a novel analytical method based on a paper based enzymatic biosensor assisted by digital image processing and first-order multivariate calibration has been reported for rapid determination of nitrate in food samples.

scholarly journals Near-Infrared Spectroscopy as a Rapid Screening Method for the Determination of Total Anthocyanin Content in Sambucus Fructus

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4983
Author(s):  
Stefan Stuppner ◽  
Sophia Mayr ◽  
Anel Beganovic ◽  
Krzysztof Beć ◽  
Justyna Grabska ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Near Infrared ◽  
Screening Method ◽  
Differential Method ◽  
Anthocyanin Content ◽  
Cyanogenic Glycosides ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Tof Ms

Elderberry (Sambucus nigra L., fructus) is a very potent herbal drug, deriving from traditional European medicine (TEM). Ripe elderberries are rich in anthocyanins, flavonols, flavonol esters, flavonol glycosides, lectins, essential oils, unsaturated fatty acids and vitamins. Nevertheless, unripe elderflower fruits contain a certain amount of sambunigrin, a toxic cyanogenic glycoside, whose concentration decreases in the ripening process. Therefore, quality assurance must be carried out. The standard method described in literature is the photometric determination (pH-differential method) of the total anthocyanin content (TAC) that is the highest when the berries are ripe. The drawback of the pH-differential method is the extensive sample preparation and the low accuracy of the method. Therefore, the goal of this publication was to develop a fast non invasive near-infrared (NIR) method for the determination of TAC in whole berries. TAC of elderberries was measured using pH-differentiation method where TAC values of 632.87 mg/kg to 4342.01 mg/kg were measured. Additionally, cyanidin-3-O-glucoside, cyanidin-3-O-sambubioside and cyanidin-3-O-sambubioside-5-O-glucoside which represent more than 98% of TAC in elderberry were quantified using ultra high performance liquid chromatography-multiple wavelength detection—ultra high resolution-quadrupole-time of flight-mass spectrometry (UHPLC-MWD-UHR-Q-TOF-MS) and their sum parameter was determined, ranging between 499.43 mg/kg and 8199.07 mg/kg. Using those two methods as reference, whole elderberries were investigated by NIR spectroscopy with the Büchi NIRFlex N-500 benchtop spectrometer. According to the constructed partial least squares regression (PLSR) models the performance was as follows: a relative standard deviation (RSDPLSR) of 13.5% and root mean square error of calibration (RMSECV/RMSEC) of 1.31 for pH-differentiation reference and a RSDPLSR of 12.9% and RMSECV/RMSEC of 1.28 for the HPLC reference method. In this study, we confirm that it is possible to perform a NIR screening for TAC in whole elderberries. Using quantum chemical calculations, we obtained detailed NIR band assignments of the analyzed compounds and interpreted the wavenumber regions established in PLSR models as meaningful for anthocyanin content. The NIR measurement turned out to be a fast and cost-efficient alternative for the determination of TAC compared to pH-differential method and UHPLC-MWD-UHR-Q-TOF-MS. Due to the benefit of no sample preparation and extraction the technology can be considered as sustainable green technology. With the above mentioned inversely proportional ratio of TAC to total amount of toxic cyanogenic glycosides, NIR proves to be a reliable screening method for the ideal harvest time with maximal content of TAC and lowest content of cyanogenic glycosides in elderberry.

scholarly journals Screening Out the Phytochemicals and Colour Values in White and Black Rice Lines and Its Interrelationship

2020 ◽  
pp. 34-41
Author(s):  
T. Vasantha Theiventhiran ◽  
S. Amutha ◽  
J. Ramalingam ◽  
G. Hemalatha ◽  
T. Umamaheswari
Keyword(s):  
Tamil Nadu ◽  
Rice Variety ◽  
Maximum Level ◽  
Differential Method ◽  
Anthocyanin Content ◽  
Black Rice ◽  
Total Anthocyanin ◽  
Rice Line ◽  
White Rice ◽  
Therapeutic Properties

Problem: Kavuni is a traditional black rice variety of Tamil Nadu, India it rich in nutritional and therapeutic properties.  These traits it is not being cultivated widely, due to long duration, poor tillering and low yielding agronomic characters. Objectives: In the  study, twenty three breeding rice lines from the cross between black kavuni and Co 50  were investigated for colour values and phytochemical viz., total phenols content (TPC), total flavonoids content (TFC) and total anthocyanin content (TAC). Methods: Twenty five rice samples were grouped as black and white rice based on colour of aleuronic layer by visual inspection. The color values expressed as L* (lightness), a* (redness), b* (yellowness), H° (hue) and C (chroma). TPC by the Folin–Ciocalteu method and expressed as milligram of gallic acid equivalent (mg GAE/g) per g. TFC by aluminum chloride method and expressed as mg catechin equivalent (mg CATE) per 100 g. TAC was measured by pH differential method, expressed as mg cyaniding 3-glucoside per 100 g (c3-glucoside/100 g). Main results: The black phenotype rice had low value in L*, b*, C, H° and high values in a* than the white color rice. In the black rice group, 145-6 breeding line had darker black aleuronic layer than other black rice. The TPC, TFC and TAC ranged between 0.74 -3.24 mg GAE/g, 12.79 – 82.58 mg CATE/100 g and 0.4-223.76 mg c3-glucoside/100 g, respectively.  High level of TPC, TFC and TAC were recorded in black phenotypic rice, where as lower levels in white rice. The maximum amount of TPC and TAC were observed in 145-6 rice line, which on par with parent black kavuni rice.  The maximum level TFC was observed in 144-2 rice line than the parent rice kavuni. The TPC, TFC and TAC were negatively correlated with L*, b*, C and H° values and positively correlated with a* values at 0.01 probability level. Among the 23 rice lines, based on TPC, TAC and TFC the superior breeding rice lines were 145-6 and 144-2 respectively. The results showed that dark black grain has higher phytochemicals than white grain. Main recommendation:  The results indicate provide the opportunities to further increase the nutritional benefits of some food products from black rice in terms of therapeutic properties.

scholarly journals STABILITAS ANTOSIANIN JANTUNG PISANG KEPOK (Musa paradisiaca L) TERHADAP CAHAYA SEBAGAI PEWARNA AGAR-AGAR

2015 ◽  
Vol 34 (04) ◽  
pp. 374 ◽  
Author(s):  
Lydia Ninan Lestario ◽  
Matius Kristiarso Wibowo Catur Yoga ◽  
Augustinus Ignatius Kristijanto
Keyword(s):  
Citric Acid ◽  
Light Intensity ◽  
Tartaric Acid ◽  
Degradation Rate ◽  
Differential Method ◽  
Anthocyanin Content ◽  
K Value ◽  
Musa Paradisiaca ◽  
Color Degradation

The objectives of this study were to determine the solution that could extract highest anthocyanin from banana bract, to determine the concentration of banana bract extract in jelly which preferred best by panelists, to determine color degradation rate in jelly colored with banana bract against light on various light intensity, and to determine the maximum light intensity which could be accepted by the panelists based on the hedonic test. Anthocyanin of banana bract was extracted by tartaric acid, citric acid, and acetate acid. Anthocyanin content of banana bract was measured by pH differential method. For the hedonic test, jelly with concentration of banana bract 15%, 20%, 25%, 30%, 40% and 50%, were used with 20 panelists. Then jelly with 25% concentration of banana bract was used for determination of color degradation rate caused by light with the intensity of 780, 1,850, 2,214 dan 10,340 lux. The results showed thatthe best solution that extracted highest anthocyanin was tartaric acid, the concentration of banana bract extract in jelly that preferred best by panelists was 25%, the color degradation rate was increased along with the increasing of light intensity, with the k value 0.0042 – 0.0096 AU/hour. After 10 hours under light with intensity of 780-2,214 lux, the jelly colored with 25 % of banana bract extract was still could be accepted by panelists, whereas under light intensity of 10,340 lux could not be accepted by panelists.Keywords: Anthocyanin, banana bract, jelly, light intensity, color degradation rate ABSTRAKTujuan dari penelitian ini adalah untuk menentukan larutan pengekstrak jantung pisang kepok yang menghasilkan antosianin tertinggi, menentukan konsentrasi ekstrak jantung pisang kepok dalam agar-agar yang paling disukai panelis, menentukan laju degradasi warna agar-agar dengan pewarna jantung pisang kepok akibat penyinaran pada berbagaiintensitas cahaya, dan menentukan intensitas cahaya maksimum yang masih dapat diterima oleh panelis berdasarkan uji hedonik setelah penyinaran. Antosianin jantung pisang kepok diekstraksi menggunakan pelarut asam tartarat, asam sitrat, dan asam asetat. Kadar antosianin diukur dengan metode perbedaan pH. Untuk uji hedonik digunakan agar-agar dengan kadar ekstrak jantung pisang kepok 15%, 20%, 25%, 30%, 40% dan 50 %. Uji stabilitas warna dilakukan terhadap agar-agar dengan pewarna jantung pisang kepok 25%, dan disinari selama 10 jam dengan intensitas cahaya 780, 1.850, 2.214, dan 10.340 lux. Hasil penelitian menunjukkan bahwa larutan pengekstrak yang menghasilkanantosianin tertinggi adalah asam tartarat. Konsentrasi ekstrak jantung pisang kepok dalam agar-agar yang paling disukai panelis adalah 25 %. Laju degradasi warna agar-agar dengan pewarna jantung pisang kepok meningkat seiring dengan meningkatnya intensitas cahaya, dengan nilai k berkisar antara 0,0042 - 0,0096 AU/Jam. Agar-agar dengan pewarnajantung pisang kepok yang disinari dengan intensitas 780-2.214 lux selama 10 jam masih disukai panelis, sedangkan yang disinari dengan intensitas 10.340 sudah tidak disukai panelis.Kata kunci: Antosianin, jantung pisang, agar-agar, intensitas cahaya, laju degradasi warna

Digital Image Analysis for the Colorimetric Determination of Aluminum, Total Iron, Nitrite and Soluble Phosphorus in Waters

2016 ◽  
Vol 50 (2) ◽  
pp. 414-430 ◽  
Author(s):  
Juan A. V. A. Barros ◽  
Fagner Moreira de Oliveira ◽  
Guilherme de O. Santos ◽  
Célio Wisniewski ◽  
Pedro Orival Luccas
Keyword(s):  
Image Analysis ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Total Iron ◽  
Colorimetric Determination ◽  
Soluble Phosphorus
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