An easily available endoplasmic reticulum targeting near-infrared fluorescent probe for esterase imaging in vitro and in vivo

The Analyst ◽  
2022 ◽  
Author(s):  
Chunbai Xiang ◽  
Jingjing Xiang ◽  
Xing Yang ◽  
Baode Zhu ◽  
Quanyi Mo ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Endoplasmic Reticulum Targeting

Here, we report an easily available endoplasmic reticulum-targeting near-infrared fluorescent probe (ER-CE), which can detect the esterase in the endoplasmic reticulum and monitor the changes of esterase amount in tumor...

A novel colorimetric and near-infrared fluorescent probe for hydrogen peroxide imaging in vitro and in vivo

RSC Advances ◽  
2015 ◽  
Vol 5 (104) ◽  
pp. 85957-85963 ◽  
Author(s):  
Peng Wang ◽  
Ke Wang ◽  
Dan Chen ◽  
Yibo Mao ◽  
Yueqing Gu
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
Near Infrared

A novel NIR fluorescent probe (DCM-B2) based on dicyanomethylene-4H-pyran was synthesized for the detection of H2O2.

A NIR fluorescent probe for the detection of fluoride ions and its application in in vivo bioimaging

2017 ◽  
Vol 5 (10) ◽  
pp. 2002-2009 ◽  
Author(s):  
Qiuyun Yang ◽  
Chunman Jia ◽  
Qing Chen ◽  
Wei Du ◽  
Yile Wang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Fluoride Ions

A near-infrared fluorescent probe has been developed, which is available for visualizing exogenous fluoride ions in vitro and in vivo.

A novel near-infrared xanthene-based fluorescent probe for detection of thiophenol in vitro and in vivo

2020 ◽  
Vol 44 (40) ◽  
pp. 17360-17367
Author(s):  
Yongquan Wu ◽  
Aiping Shi ◽  
Huiying Liu ◽  
Yuanyan Li ◽  
Weican Lun ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Living Cells

A novel near-infrared xanthene-based fluorescent probe for detection of thiophenol in living cells and mice.

An RGD modified water-soluble fluorophore probe for in vivo NIR-II imaging of thrombosis

2020 ◽  
Vol 8 (16) ◽  
pp. 4438-4446
Author(s):  
Yanping Wu ◽  
Chao Wang ◽  
Jiaqi Guo ◽  
Abdlay Carvalho ◽  
Yuyu Yao ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Water Soluble

Synthesis of an organic near-infrared second-window fluorescent probe targeting in vivo and in vitro thrombi.

Near-Infrared Fluorescent Probe Activated by Nitroreductase for In Vitro and In Vivo Hypoxic Tumor Detection

2021 ◽  
Author(s):  
Sanu Karan ◽  
Mi Young Cho ◽  
Hyunseung Lee ◽  
Hwunjae Lee ◽  
Hye Sun Park ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Tumor Detection ◽  
Hypoxic Tumor

A novel fibroblast activation protein-targeted near-infrared fluorescent off–on probe for cancer cell detection, in vitro and in vivo imaging

2018 ◽  
Vol 6 (10) ◽  
pp. 1449-1451 ◽  
Author(s):  
Jie Xing ◽  
Qiuyu Gong ◽  
Ruifen Zou ◽  
Zihou Li ◽  
Yuanzhi Xia ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fibroblast Activation Protein ◽  
Cell Detection ◽  
Fibroblast Activation ◽  
Design And Synthesis ◽  
Cancer Cell Detection

Design and synthesis of a novel fibroblast activation protein “off–on” near-infrared fluorescent probe for cell detection, in vitro and in vivo imaging.

Abstract 156: Development of a New Bioactivatable Fluorescent Probe for the Study of the Proteolytic Activities Degrading Apolipoprotein A-I in vitro and in vivo

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Foued Maafi ◽  
Baoqiang Li ◽  
Catherine Gebhard ◽  
Mathieu Brodeur ◽  
Louis Villeneuve ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Protease Inhibitors ◽  
Near Infrared ◽  
Ex Vivo ◽  
Fold Increase ◽  
High Density Lipoproteins ◽  
Apolipoprotein A ◽  
Proteolytic Activities

Introduction and Objective: The possible benefits of high-density lipoproteins (HDL) against atherosclerosis have been largely attributed to its major protein component, apolipoprotein A-I (apoA-I). However, apoA-I can be degraded by diverse processes, including proteases localized in atherosclerotic plaques, which could reduce the effectiveness of HDL-based therapies. Here we describe the development of a new bioactivatable near-infrared apoA-I-Cy5.5 fluorescent probe and its initial use in the assessment of proteolytic activities that degrade apoA-I in vitro, in vivo and ex vivo. Methods and Results: Fluorescence emission of our probe is quenched by saturation of Cy5.5 fluorophore molecules on the full-length apoA-I protein. In vitro proteolysis of the apoA-I probe showed up to 11-fold increase of near-infrared fluorescence (n=5, P ≤ 0.05). Using this apoA-I-Cy5.5 probe, we were able to quantify proteolytic activities from a wide range of proteases targeting serine (chymase), cysteine (cathepsin S) and metalloproteases (MMP-12). Also, we detected activation of the apoA-I-Cy5.5 probe on aortic cryosections from Ldlr-/--Tg for human apoB atherosclerotic (ATX) mice using an in situ zymography assay and observed that broad-spectrum protease inhibitors protect the probe from protease activities, as shown by decreased fluorescence compared to conditions without protease inhibitors (-54%, n= 6 per group, P ≤ 0.001). In vivo, using a combined Fluorescence Molecular Tomography-Magnetic Resonance imaging system, the injected probe exhibited a trend for increased fluorescence in the aorta when infused in ATX mice compared to C57BL/6J wild-type mice. Ex vivo imaging of these aortas showed a 10-fold increase of fluorescence in ATX (n=5) mice compared to CTL (n=3) mice (P ≤ 0.05). Conclusion: Given the potential importance of HDL functionality in the assessment of cardiovascular risk, this novel protease-activatable apoA-I probe may help to improve HDL-based therapies through better characterization of the alterations of functionality of apoA-I or lipid-poor HDL particles in different pathophysiological settings.

A near-infrared multifunctional fluorescent probe with an inherent tumor-targeting property for bioimaging

2015 ◽  
Vol 51 (58) ◽  
pp. 11721-11724 ◽  
Author(s):  
Xu Zhao ◽  
Yang Li ◽  
Di Jin ◽  
Yuzhi Xing ◽  
Xilong Yan ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Tumor Targeting ◽  
Near Infrared ◽  
Mitochondria Targeting

A mitochondria-targeting near-infrared switchable probe for in vitro and in vivo tumor-targeting imaging.

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