Gas pressure-assisted ratiometric atomic flame assay for point-of-care testing of tumor cell-derived exosomes

The Analyst ◽  
2021 ◽  
Author(s):  
Shengqiang Hu ◽  
Xue ting Fang ◽  
Guijing Liu ◽  
Guixiang Ma ◽  
Fanggui Ye ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Tumor Cell ◽  
Point Of Care ◽  
Resonance Energy ◽  
Gas Pressure ◽  
Point Of Care Testing ◽  
Vital Importance ◽  
Fluorescence Resonance ◽  
Tumor Growth And Metastasis

Multicolor-based point-of-care testing (POCT) of tumor cell-derived exosomes is of vital importance for understanding tumor growth and metastasis. Multicolor-based ratiometric signals focused on molecular optics, such as fluorescence resonance energy...

Point-of-care testing of melamine via gas pressure readout using polythymine-coated Au@Pt nanoparticles through specific triple hydrogen-bonding recognition

The Analyst ◽  
2021 ◽  
Author(s):  
Ling Li ◽  
Hanwen Deng ◽  
Zhongshuai Zhao ◽  
Zhongde Liu
Keyword(s):  
Hydrogen Bonding ◽  
Point Of Care ◽  
Pt Nanoparticles ◽  
Gas Pressure ◽  
Point Of Care Testing ◽  
Highly Sensitive

On the basis of the target-responsive aggregation occurrence of Au@PtNPs, a pressure-based signaling strategy was developed for highly sensitive and specific melamine detection not only in laboratory but also in point-of-care (POC) settings.

scholarly journals Inhibition of endothelial FAK activity prevents tumor metastasis by enhancing barrier function

2014 ◽  
Vol 204 (2) ◽  
pp. 247-263 ◽  
Author(s):  
Christine Jean ◽  
Xiao Lei Chen ◽  
Ju-Ock Nam ◽  
Isabelle Tancioni ◽  
Sean Uryu ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Tumor Cell ◽  
Stromal Cells ◽  
Barrier Function ◽  
Tumor Metastasis ◽  
Vascular Endothelial ◽  
Vascular Endothelial Cadherin ◽  
Tumor Growth And Metastasis ◽  
Endothelial Growth Factor

Pharmacological focal adhesion kinase (FAK) inhibition prevents tumor growth and metastasis, via actions on both tumor and stromal cells. In this paper, we show that vascular endothelial cadherin (VEC) tyrosine (Y) 658 is a target of FAK in tumor-associated endothelial cells (ECs). Conditional kinase-dead FAK knockin within ECs inhibited recombinant vascular endothelial growth factor (VEGF-A) and tumor-induced VEC-Y658 phosphorylation in vivo. Adherence of VEGF-expressing tumor cells to ECs triggered FAK-dependent VEC-Y658 phosphorylation. Both FAK inhibition and VEC-Y658F mutation within ECs prevented VEGF-initiated paracellular permeability and tumor cell transmigration across EC barriers. In mice, EC FAK inhibition prevented VEGF-dependent tumor cell extravasation and melanoma dermal to lung metastasis without affecting primary tumor growth. As pharmacological c-Src or FAK inhibition prevents VEGF-stimulated c-Src and FAK translocation to EC adherens junctions, but FAK inhibition does not alter c-Src activation, our experiments identify EC FAK as a key intermediate between c-Src and the regulation of EC barrier function controlling tumor metastasis.

scholarly journals A novel quantification platform for point-of-care testing of circulating MicroRNAs based on allosteric spherical nanoprobe

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Huiyan Tian ◽  
Changjing Yuan ◽  
Yu Liu ◽  
Zhi Li ◽  
Ke Xia ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Reaction Cross ◽  
Detection Sensitivity ◽  
Point Of Care Testing ◽  
Bioanalytical Application ◽  
Target Probe ◽  
Nsclc Patients

Abstract MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer (NSCLC), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC. Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield “signal on” fluorescence. In the manner of multipoint fluorescence detection, the target-bound allosteric spherical nanoprobe could provide high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method can distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

scholarly journals A Novel Quantification Platform for Point-of-care testing of Circulating MicroRNAs Based on Allosteric Spherical Nanoprobe

2020 ◽  
Author(s):  
Huiyan Tian ◽  
Changjing Yuan ◽  
Yu Liu ◽  
Zhi Li ◽  
Ke Xia ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Reaction Cross ◽  
Detection Sensitivity ◽  
Point Of Care Testing ◽  
Bioanalytical Application ◽  
Target Probe ◽  
Nsclc Patients

Abstract MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer ( NSCLC ), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC . Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield a “signal on” fluorescence. In the manner of multipoint fluorescence detection , the target-bound allosteric spherical nanoprobe could provide a high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method could distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

scholarly journals Time-resolved Fluorescence Resonance Energy Transfer Assay for Point-of-Care Testing of Urinary Albumin

2003 ◽  
Vol 49 (7) ◽  
pp. 1105-1113 ◽  
Author(s):  
Qiu-Ping Qin ◽  
Olli Peltola ◽  
Kim Pettersson
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Time Resolved ◽  
Urine Albumin ◽  
Europium Chelate ◽  
Fluorescence Resonance

Abstract Background: Microalbuminuria is an established early marker of diabetic nephropathy and an important cardiovascular risk factor in diabetes and hypertension. We aimed to develop a rapid point-of-care assay for the measurement of urine albumin. Methods: The competitive homogeneous assay used an albumin-specific monoclonal antibody labeled with a stable fluorescent europium chelate as donor and an albumin labeled with cyanine 5 (Cy5) as acceptor. The assay was performed at room temperature in single microtitration wells that contained all the required dry-form reagents. The close proximity between the two labels in the immune complex allowed fluorescence resonance energy to be transferred from the pulse-excited europium chelate to the acceptor Cy5. The emission of long-lived energy transfer signal from the sensitized Cy5 was measured at 665 nm with time-resolved fluorometry that eliminated short-lived background. Results: The assay procedure required 12 min for a 10-μL urine sample. The working range was from 10 to ∼320 mg/L, and the lower limit of detection was 5.5 mg/L. The within- and between-run CVs were 6.9–10% and 7.5–13%, respectively. Recovery was 103–122%. The assay correlated well (r2 = 0.98; n = 37) with a laboratory-based immunoassay, although mean (SD) results were 7 (29)% lower. Conclusions: The speed and ease of performance of this assay recommend it for near-patient use. The assay is the first to combine a fluorescence resonance energy transfer-type rapid competitive assay with an all-in-one dry reagent.

scholarly journals Tumor cell surface heparan sulfate as cryptic promoters or inhibitors of tumor growth and metastasis

2002 ◽  
Vol 99 (2) ◽  
pp. 568-573 ◽  
Author(s):  
D. Liu ◽  
Z. Shriver ◽  
G. Venkataraman ◽  
Y. El Shabrawi ◽  
R. Sasisekharan
Keyword(s):  
Cell Surface ◽  
Tumor Growth ◽  
Tumor Cell ◽  
Heparan Sulfate ◽  
Tumor Growth And Metastasis ◽  
Cryptic Promoters

scholarly journals A Sample-to-Answer Quantification Platform for Point-of-Care Testing of Circulating MicroRNAs Based on Allosteric Spherical Nanoprobe

2020 ◽  
Author(s):  
Huiyan Tian ◽  
Changjing Yuan ◽  
Yu Liu ◽  
Ke Xia ◽  
Mengya Li ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Reaction Cross ◽  
Detection Sensitivity ◽  
Point Of Care Testing ◽  
Bioanalytical Application ◽  
Target Probe ◽  
Nsclc Patients

Abstract MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer (NSCLC), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC. Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield a “signal on” fluorescence. In the manner of multipoint fluorescence detection, the target-bound allosteric spherical nanoprobe could provide a high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method can distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

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