De novo structural determination of oligosaccharide isomers in glycosphingolipids using logically derived sequence tandem mass spectrometry

The Analyst ◽  
2021 ◽  
Author(s):  
Chia Yen Liew ◽  
Chieh-Kai Chan ◽  
Shih-Pei Huang ◽  
Yu Ting Cheng ◽  
Shang-Ting Tsai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
De Novo ◽  
Biological Systems ◽  
Tandem Mass ◽  
Structural Determination

Despite the importance of carbohydrates for biological systems, structural determination of carbohydrates remains difficult because of large number of isomers. In this study, a new mass spectrometry, namely logically derived...

De novo structural determination of mannose oligosaccharides by using a logically derived sequence for tandem mass spectrometry

2019 ◽  
Vol 411 (15) ◽  
pp. 3241-3255 ◽  
Author(s):  
Hsu Chen Hsu ◽  
Shih-Pei Huang ◽  
Chia Yen Liew ◽  
Shang-Ting Tsai ◽  
Chi-Kung Ni
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
De Novo ◽  
Tandem Mass ◽  
Structural Determination

Structural determination of cyclitol derivatives by fast-atom bombardment tandem mass spectrometry

2006 ◽  
Vol 20 (8) ◽  
pp. 1253-1256 ◽  
Author(s):  
Ji Hye Gil ◽  
JungJu Seo ◽  
Kang-Jin Kim ◽  
Jee H. Jung ◽  
Ok-Sang Jung ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Fast Atom Bombardment ◽  
Tandem Mass ◽  
Structural Determination

Automatically Full Glycan Structural Determination with Logically Derived Sequence Tandem Mass Spectrometry

2019 ◽  
Author(s):  
Shang-Ting Tsai ◽  
Chia Yen Liew ◽  
Hsu Chen Hsu ◽  
Shih-Pei Huang ◽  
Wei-Chien Weng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
De Novo ◽  
Bovine Milk ◽  
High Sensitivity ◽  
Tandem Mass ◽  
Structural Determination ◽  
Spectrum Measurement ◽  
Linkage Position

Here we show the full structures of glycans, including diastereomers and anomericity of each monosaccharide and linkage position of each glycosidic bond, can be determined using tandem mass spectrometry guided by a logically derived sequence (LODES). This new method provides de novo oligosaccharide structural identifications with high sensitivity and was applied to automatically in situ structural determination of the oligosaccharides eluted by high performance liquid chromatography. We showed that the structure of a given trisaccharide from trisaccharide mixture and bovine milk were determined from near three thousand isomers by using six to seven logically selected CID spectra. The entire procedure of mass spectrum measurement guided by LODES can be programmed in computer for automatically full glycan structural identification, a goal that remains a great challenge in glycan analysis.

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