Wash-free Paper Diagnostics for the Rapid Detection of Blood Type Antibodies

The Analyst ◽  
2021 ◽  
Author(s):  
Michael J Hertaeg ◽  
Vidhishri Kesarawani ◽  
Heather McLiesh ◽  
Julia Walker ◽  
Simon Robert Corrie ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Rapid Detection ◽  
Diagnostic Procedures ◽  
Blood Type ◽  
Free Paper ◽  
Specific Antibodies ◽  
Paper Diagnostics ◽  
Safe Blood

Identification of specific antibodies in patient plasma is an essential part of many diagnostic procedures and is critical for safe blood transfusion. Current techniques require laboratory infrastructure and long turnaround...

Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum

2014 ◽  
Vol 63 (2) ◽  
pp. 309-312 ◽  
Author(s):  
Georg Härter ◽  
Hagen Frickmann ◽  
Sebastian Zenk ◽  
Dominic Wichmann ◽  
Bettina Ammann ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Symptoms ◽  
Diagnostic Procedures ◽  
Lower Limbs ◽  
Antibody Specificity ◽  
Quantitative Real Time Pcr ◽  
Specific Antibodies ◽  
Routine Diagnosis

We describe the case of a 16-year-old German male expatriate from Ghana who presented with obstipation, dysuria, dysaesthesia of the gluteal region and the lower limbs, bilateral plantar hypaesthesia and paraesthesia without pareses. A serum–cerebrospinal fluid (CSF) Schistosoma spp. specific antibody specificity index of 3.1 was considered highly suggestive of intrathecal synthesis of anti-Schistosoma spp. specific antibodies, although standardization of this procedure has not previously been described. Diagnosis was confirmed by detection of Schistosoma DNA in CSF by semi-quantitative real-time PCR at 100-fold concentration compared with serum. Accordingly the two diagnostic procedures, which have not previously been applied for routine diagnosis, appear to be useful for the diagnosis of neuroschistosomiasis. Clinical symptoms resolved following anthelmintic and anti-inflammatory therapy.

scholarly journals Gambaran Hasil Pemeriksaan HIV pada Darah Donor

2021 ◽  
Vol 2 (1) ◽  
pp. 46-50
Author(s):  
Bagus Triatmojo
Keyword(s):  
Blood Transfusion ◽  
Age Groups ◽  
Blood Type ◽  
Blood Products ◽  
Blood Safety ◽  
Elisa Method ◽  
Donor Blood ◽  
Voluntary Donor ◽  
Filter Test ◽  
Research Type

HIV cases in Jepara District in recent years have demanded the handling of several aspects simultaneously including the Blood Transfusion Unit (BTU). HIV can be transmitted through blood transfusion or other blood products. BTU of PMI Jepara Regency carries out recruitment of voluntary donor from Jepara society. The HIV examination as part of an IMLTD screening test is done for safety of donor blood. The HIV examination in all donor blood uses rapid and elisa method. Examination results are stated as reactive and non reactive. Research objective to find out the description of HIV examination results in donor blood at the BTU of PMI Kabupaten Jepara in 2019. Research method the research type was descriptive using records of donor data at BTU of PMI Kabupaten Jepara in 2019. The examination results of reactive HIV in 12 donor blood (0,08%). non-reactive blood donor of HIV  in age groups 18 years, 18-24, 25-44, 45-59, and ≥ 60 years respectively 9,55%, 22,39%, 51,75%, 16,01% and 0,21%. Reactive HIV in age groups 18-24, 25-44, and 45-59 years were 0,034%, 0,034%, and 0,13%. Non-reactive HIV in men 68,22% and in women 31,70%. Reactive HIV in men 0,07%, and women 0,01%. Non-reactive HIV in voluntary donor blood was 99,779% and substitute donor was 0,127%. HIV of reactive voluntary donor blood was 0,074%, and substitute donor was 0,007%. HIV filter test is indispensable for blood safety because the age, gender and blood type of donors have the potential for reactive HIV.

scholarly journals Haemovigilance: New approach for safe Blood transfusion

2019 ◽  
Vol 7 (4) ◽  
pp. 1-7
Author(s):  
Sravya Sree ◽  
Pasikhanti Shailaja
Keyword(s):  
Blood Transfusion ◽  
Adverse Reactions ◽  
Uttar Pradesh ◽  
Primary Objective ◽  
Family Welfare ◽  
Blood Collection ◽  
Blood Products ◽  
Preventive Actions ◽  
Potential Risks ◽  
Safe Blood

Haemovigilance is an urgent need of the country to identify and prevent occurrence or recurrence of transfusion related adverse reactions, thereby to increase the safety and quality of blood transfusion and blood products administration. Haemovigilance is an organised scheme of monitoring, identifying, reporting, investigating and analysing adverse events and reactions pertinent to transfusion and manufacturing blood products. Thus the information collected will facilitate corrective and preventive actions to minimise the potential risks associated with blood collection, processing and transfusion to patients. Indian Pharmacopoeia Commission has started a Haemovigilance Program of India (HvPI) in 2012 under its Pharmacovigilance Program of India (PvPI) in collaboration with National Institute of Biologicals (NIB), Noida, Uttar Pradesh, under Ministry of Health and Family welfare, Government of India with a primary objective to track adverse reactions/events and incidences associated with blood transfusion and blood product administration. The main objective of this article is to brief (s) about the system which monitors each and every step of transfusion reaction.

A framework to support safe blood transfusion practice

2010 ◽  
Vol 24 (48) ◽  
pp. 35-40 ◽  
Author(s):  
Elizabeth Pirie ◽  
Jan Green
Keyword(s):  
Blood Transfusion ◽  
Transfusion Practice ◽  
Safe Blood

A framework to support safe blood transfusion practice

2010 ◽  
Vol 24 (48) ◽  
pp. 35-40
Author(s):  
Elizabeth Pirie ◽  
Jan Green
Keyword(s):  
Blood Transfusion ◽  
Transfusion Practice ◽  
Safe Blood

Red Blood Cell Exchange Transfusion (RBCET) in the Management of Acute Hemolytic Transfusion Reaction (AHTR).

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4021-4021 ◽  
Author(s):  
Shelonitda S. Rose ◽  
Simi George ◽  
Serena Wong ◽  
Grace Tenorino ◽  
Mercy Kuriyan
Keyword(s):  
Coronary Artery ◽  
Blood Transfusion ◽  
Serum Creatinine ◽  
Supportive Care ◽  
Blood Cells ◽  
Urine Output ◽  
Type A ◽  
Blood Type ◽  
Transfusion Reaction ◽  
Hemolytic Transfusion Reaction

Abstract Acute hemolytic transfusion reaction (AHTR) is a potentially serious complication associated with the transfusion of ABO incompatible blood. It results in disseminated intravascular coagulation (DIC), shock, renal failure and death. Patient misidentification, sample mislabeling are some of the causes of an AHTR. To-date there is no data available on the use of RBCET in the management of an AHTR. We report the clinical course and successful management of an AHTR following massive ABO incompatible blood transfusion. The patient is a 68-year-old female with known coronary artery disease and type O Rh negative blood, who presented to the emergency room with unstable angina. She was taken emergently to the operating room for a quadruple coronary artery bypass graft. Due to sample mislabeling, she received 7 units of incompatible type A Rh negative packed red blood cells (PRBC) intra-operatively. During the immediate post-operative period, she was noted to have increased drainage from the surgical site, decreased urine output, hematuria and a precipitous drop in hemoglobin to 6.5 g/dL. She underwent re-exploration and received an additional 2 units of incompatible type A Rh negative PRBC and other blood products including fresh frozen plasma (FFP), platelets and cryoprecipitate that were ABO incompatible. In the recovery room, family members confirmed her blood type as type O Rh negative. Immediate re-typing utilizing pre-transfusion blood samples collected for CBC and chemistry, and obtaining blood bank records from her previous admission from another hospital a diagnosis of ABO incompatible AHTR was established. Further work-up revealed a strong positive DAT (3+ IgG) with a positive eluate (patient’s anti-A antibodies coating the transfused type A RBC’s). Hemolysis parameters showed elevated LDH 753 IU, bilirubin 2.1 mg/dL, and decreased haptoglobin 29.4 mg/dL. Her urine output continued to decrease with increase in serum creatinine to 2.4mg/dL. A continuous veno-venous hemodialysis (CVVHD) was started along with fluid resuscitation and urine alkalinization. A RBCET was performed within 11 hours after the transfusion of the last incompatible unit, which was followed by a second RBCET 24 hours later. Each RBCET consisted of 8 units of type O Rh negative blood. Following the two RBCET her blood type became type O Rh negative. Hemolysis parameters improved over the next several days and became normal. Serum creatinine progressively improved with increasing urine output. Dialysis was discontinued on day 13. Patient continued to do well and was discharged home in good condition with a normal renal function three weeks after her initial admission. The severity and course of an AHTR is dictated by the load of incompatible blood cells in the circulation. The high mortality rate associated with an AHTR warrants an aggressive approach in addition to supportive care. RBCET is used in the management of severe hemolytic disease of the new born, complications of sickle cell disease, and to lower the parasite load in malaria and babesiosis. The role of RBCET in AHTR is not established. There are two case reports in the Japanese literature where a RBCET was performed in the management of an AHTR with successful outcome. The course of this patient suggests that aggressive management including supportive care, timely RBCET, and renal replacement treatment may prevent DIC, organ damage and alter the grave prognosis associated with AHTR following incompatible blood transfusion.

Examining Knowledge and Information Seeking Behaviors towards Blood Transfusion Among Individuals with Cancer

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 4762-4762
Author(s):  
Ahmad B Naim ◽  
Danielle Walls ◽  
Jan Gollins ◽  
Chuck Reynolds
Keyword(s):  
Blood Transfusion ◽  
Information Seeking ◽  
Online Survey ◽  
Informed Choice ◽  
Blood Type ◽  
Blood Transfusions ◽  
Sources Of Information ◽  
Nationally Representative ◽  
Doctor's Office ◽  
Information Seeking Behaviors

Abstract Abstract 4762 Objective: Examine knowledge and information seeking behaviors towards blood transfusions among individuals with metastatic or recurrent cancer. Methods: An online survey was conducted from a nationally representative patient panel in 1Q2011. All respondents were ≥18 years and diagnosed with cancer by a physician. Participants were asked about their blood transfusion history, information seeking behaviors, and knowledge about blood transfusion. Results: 206 individuals responded to survey. 65% (n=133) were female and 25% (n=52) were over 55 years. 55% (n=114) were anemic and 45% (n=92) not anemic. 62% (n=128) had received blood transfusion, whereas, 38% (n=78) had no transfusions. Top two sources of information are doctor (85%) and Internet (78%). Among those previously transfused, 74% received right amount of information, whereas, 11% received too little information, and 15% received too much information. More than 90% of transfused indicated they knew the reasons for and benefits of getting a blood transfusion. Less than two-thirds received information about the costs. Over 65% of not transfused said that it is extremely important to know risks of infections, right blood type and screening techniques. Among previously transfused, about 70% agreed that they made an informed choice about receiving blood transfusions. Among the previously transfused, 78% agree that they knew the benefits compared with 68% not transfused. About 68% of transfused and not transfused agree they knew the risks of blood transfusion. Conclusions: Doctor's office and internet are primary sources of information about blood transfusions. Gaps in knowledge exist about benefits, risks, and costs of blood transfusions. A significant number feel that they need more information about blood transfusion to make an informed choice. Providers should consider adopting shared-decision making with their patients. Disclosures: Naim: Janssen Scientific Affairs, LLC: Employment. Walls:Janssen Scientific Affairs, LLC: Consultancy. Gollins:Janssen Scientific Affairs, LLC: Consultancy. Reynolds:Janssen Scientific Affairs, LLC: Consultancy.

scholarly journals Safe Blood Transfusion Process Improvement

2015 ◽  
Vol 144 (suppl 2) ◽  
pp. A197-A197
Author(s):  
Cindy Parrish ◽  
Sarah Long ◽  
Kevin Dryanski
Keyword(s):  
Blood Transfusion ◽  
Process Improvement ◽  
Safe Blood
Sign in / Sign up

Export Citation Format

Share Document