scholarly journals Protective Mechanism of Dried Blood Spheroids: Stabilization of Labile Analytes in Whole Blood, Plasma, and Serum

The Analyst ◽  
2021 ◽  
Author(s):  
Benjamin Frey ◽  
Deidre E Damon ◽  
Danyelle M. Allen ◽  
Jill Baker ◽  
Abraham K. Badu-Tawiah ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Whole Blood ◽  
Three Dimensional ◽  
Ambient Air ◽  
Protective Mechanism ◽  
Hydrophobic Paper

Three-dimensional (3D) dried blood spheroids form when whole blood is deposited onto hydrophobic paper and allowed to dry in ambient air. The adsorbed 3D dried blood spheroid present at the...

Comparison of whole blood, plasma and nails as monitors for the dietary intake of selenium

1998 ◽  
Vol 236 (1-2) ◽  
pp. 29-34 ◽  
Author(s):  
M. M. Mason ◽  
J. S. Morris ◽  
V. L. Spate ◽  
C. K. Baskett ◽  
T. A. Nichols ◽  
...  
Keyword(s):  
Dietary Intake ◽  
Blood Plasma ◽  
Whole Blood

scholarly journals Effect of the cholesterol content of small unilamellar liposomes on their stability in vivo and in vitro

1980 ◽  
Vol 186 (2) ◽  
pp. 591-598 ◽  
Author(s):  
Christopher Kirby ◽  
Jacqui Clarke ◽  
Gregory Gregoriadis
Keyword(s):  
Blood Plasma ◽  
Intravenous Injection ◽  
Whole Blood ◽  
Cholesterol Content ◽  
Molar Ratio ◽  
Phosphatidylcholine Liposomes ◽  
Effective Use ◽  
Positively Charged

Small unilamellar neutral, negatively and positively charged liposomes composed of egg phosphatidylcholine, various amounts of cholesterol and, when appropriate, phosphatidic acid or stearylamine and containing 6-carboxyfluorescein were injected into mice, incubated with mouse whole blood, plasma or serum or stored at 4°C. Liposomal stability, i.e. the extent to which 6-carboxyfluorescein is retained by liposomes, was dependent on their cholesterol content. (1) Cholesterol-rich (egg phosphatidylcholine/cholesterol, 7:7 molar ratio) liposomes, regardless of surface charge, remained stable in the blood of intravenously injected animals for up to at least 400min. In addition, stability of cholesterol-rich liposomes was largely maintained in vitro in the presence of whole blood, plasma or serum for at least 90min. (2) Cholesterol-poor (egg phosphatidylcholine/cholesterol, 7:2 molar ratio) or cholesterol-free (egg phosphatidylcholine) liposomes lost very rapidly (at most within 2min) much of their stability after intravenous injection or upon contact with whole blood, plasma or serum. Whole blood and to some extent plasma were less detrimental to stability than was serum. (3) After intraperitoneal injection, neutral cholesterol-rich liposomes survived in the peritoneal cavity to enter the blood circulation in their intact form. Liposomes injected intramuscularly also entered the circulation, although with somewhat diminished stability. (4) Stability of neutral and negatively charged cholesterol-rich liposomes stored at 4°C was maintained for several days, and by 53 days it had declined only moderately. Stored liposomes retained their unilamellar structure and their ability to remain stable in the blood after intravenous injection. (5) Control of liposomal stability by adjusting their cholesterol content may help in the design of liposomes for effective use in biological systems in vivo and in vitro.

scholarly journals In-line whole blood fractionation for Raman analysis of blood plasma

The Analyst ◽  
2019 ◽  
Vol 144 (2) ◽  
pp. 602-610 ◽  
Author(s):  
Moritz Matthiae ◽  
Xiaolong Zhu ◽  
Rodolphe Marie ◽  
Anders Kristensen
Keyword(s):  
Blood Flow ◽  
Blood Plasma ◽  
Whole Blood ◽  
Raman Analysis ◽  
Free Plasma

Raman studies of dynamically expanded cell-free plasma domains in microfluidic blood flow.

scholarly journals Three-Dimensional High-Resolution Digital Inline Hologram Reconstruction with a Volumetric Deconvolution Method

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 2918 ◽  
Author(s):  
Junseong Eom ◽  
Sangjun Moon
Keyword(s):  
High Resolution ◽  
Whole Blood ◽  
Tissue Sample ◽  
Three Dimensional ◽  
Kidney Tissue ◽  
Wide Field ◽  
Deconvolution Technique ◽  
Micron Size ◽  
Volumetric Images ◽  
Focus Image

The digital in-line holographic microscope (DIHM) was developed for a 2D imaging technology and has recently been adapted to 3D imaging methods, providing new approaches to obtaining volumetric images with both a high resolution and wide field-of-view (FOV), which allows the physical limitations to be overcome. However, during the sectioning process of 3D image generation, the out-of-focus image of the object becomes a significant impediment to obtaining evident 3D features in the 2D sectioning plane of a thick biological sample. Based on phase retrieved high-resolution holographic imaging and a 3D deconvolution technique, we demonstrate that a high-resolution 3D volumetric image, which significantly reduces wave-front reconstruction and out-of-focus artifacts, can be achieved. The results show a 3D volumetric image that is more finely focused compared to a conventional 3D stacked image from 2D reconstructed images in relation to micron-size polystyrene beads, a whole blood smear, and a kidney tissue sample. We believe that this technology can be applicable for medical-grade images of smeared whole blood or an optically cleared tissue sample for mobile phytological microscopy and laser sectioning microscopy.

Sign in / Sign up

Export Citation Format

Share Document