Comparison of whole blood, plasma and nails as monitors for the dietary intake of selenium

1998 ◽  
Vol 236 (1-2) ◽  
pp. 29-34 ◽  
Author(s):  
M. M. Mason ◽  
J. S. Morris ◽  
V. L. Spate ◽  
C. K. Baskett ◽  
T. A. Nichols ◽  
...  
Keyword(s):  
Dietary Intake ◽  
Blood Plasma ◽  
Whole Blood

scholarly journals Thiamine Diphosphate Status and Dialysis-Related Losses in End-Stage Kidney Disease Patients Treated with Hemodialysis

2017 ◽  
Vol 44 (4) ◽  
pp. 294-300 ◽  
Author(s):  
Magdalena Jankowska ◽  
Paweł Rudnicki-Velasquez ◽  
Hanna Storoniak ◽  
Przemysław Rutkowski ◽  
Bolesław Rutkowski ◽  
...  
Keyword(s):  
Body Weight ◽  
Kidney Disease ◽  
Dietary Intake ◽  
Whole Blood ◽  
Vitamin B1 ◽  
Thiamine Diphosphate ◽  
End Stage Kidney Disease ◽  
Before And After ◽  
End Stage ◽  
The Impact

Aim: (1) To describe the whole blood content of thiamine diphosphate (TDP), a biologically active form of vitamin B1 in end-stage kidney disease patients treated with hemodialysis (HD); (2) to establish the impact of a single HD procedure on TDP blood concentrations; and (3) to describe potential explanatory variables influencing TDP dialysis related losses, including dialysis prescription, vitamin B1 dietary intake and supplementation. Methods: Single-center, cross-sectional study in 50 clinically stable maintenance HD patients. The assessment of whole blood TDP with the High Performance Liquid Chromatography method, before and after a single, middle-week dialysis session and analysis of clinical and laboratory parameters potentially influencing TDP status Results: We report a significant difference in TDP levels before and after HD sessions - 42.5 (95% CI 38.7-46.2) μg/L and 23.6 (95% CI 18.9-28.2) μg/L, respectively (p = 0.000). The magnitude of intradialytic TDP changes is highly variable among individuals and is negatively associated only with the body weight of the patients (p < 0.013). Vitamin B1 dietary intake and supplementation do not influence whole blood TDP and dialysis-related loss of TDP. Conclusions: TDP, a bioactive compound of vitamin B1, is substantially lost during the HD procedure, and the magnitude of its loss is associated with the patient's body weight but it is not influenced by vitamin B1 dietary intake and standard supplementation dose.

scholarly journals Effect of the cholesterol content of small unilamellar liposomes on their stability in vivo and in vitro

1980 ◽  
Vol 186 (2) ◽  
pp. 591-598 ◽  
Author(s):  
Christopher Kirby ◽  
Jacqui Clarke ◽  
Gregory Gregoriadis
Keyword(s):  
Blood Plasma ◽  
Intravenous Injection ◽  
Whole Blood ◽  
Cholesterol Content ◽  
Molar Ratio ◽  
Phosphatidylcholine Liposomes ◽  
Effective Use ◽  
Positively Charged

Small unilamellar neutral, negatively and positively charged liposomes composed of egg phosphatidylcholine, various amounts of cholesterol and, when appropriate, phosphatidic acid or stearylamine and containing 6-carboxyfluorescein were injected into mice, incubated with mouse whole blood, plasma or serum or stored at 4°C. Liposomal stability, i.e. the extent to which 6-carboxyfluorescein is retained by liposomes, was dependent on their cholesterol content. (1) Cholesterol-rich (egg phosphatidylcholine/cholesterol, 7:7 molar ratio) liposomes, regardless of surface charge, remained stable in the blood of intravenously injected animals for up to at least 400min. In addition, stability of cholesterol-rich liposomes was largely maintained in vitro in the presence of whole blood, plasma or serum for at least 90min. (2) Cholesterol-poor (egg phosphatidylcholine/cholesterol, 7:2 molar ratio) or cholesterol-free (egg phosphatidylcholine) liposomes lost very rapidly (at most within 2min) much of their stability after intravenous injection or upon contact with whole blood, plasma or serum. Whole blood and to some extent plasma were less detrimental to stability than was serum. (3) After intraperitoneal injection, neutral cholesterol-rich liposomes survived in the peritoneal cavity to enter the blood circulation in their intact form. Liposomes injected intramuscularly also entered the circulation, although with somewhat diminished stability. (4) Stability of neutral and negatively charged cholesterol-rich liposomes stored at 4°C was maintained for several days, and by 53 days it had declined only moderately. Stored liposomes retained their unilamellar structure and their ability to remain stable in the blood after intravenous injection. (5) Control of liposomal stability by adjusting their cholesterol content may help in the design of liposomes for effective use in biological systems in vivo and in vitro.

scholarly journals In-line whole blood fractionation for Raman analysis of blood plasma

The Analyst ◽  
2019 ◽  
Vol 144 (2) ◽  
pp. 602-610 ◽  
Author(s):  
Moritz Matthiae ◽  
Xiaolong Zhu ◽  
Rodolphe Marie ◽  
Anders Kristensen
Keyword(s):  
Blood Flow ◽  
Blood Plasma ◽  
Whole Blood ◽  
Raman Analysis ◽  
Free Plasma

Raman studies of dynamically expanded cell-free plasma domains in microfluidic blood flow.

scholarly journals Reality and perspective of instrumental diagnostics hemostasis system functional state in critical medicine

2009 ◽  
Vol 8 (4(2)) ◽  
pp. 189-193
Author(s):  
M. N. Shpisman ◽  
I. I. Tyutrin ◽  
V. V. Udut ◽  
Ye. G. Ripp ◽  
V. O. Sorokozsherdiyev
Keyword(s):  
Blood Plasma ◽  
Functional State ◽  
Whole Blood ◽  
Critical State ◽  
Plasma Cells ◽  
Diagnostic Value ◽  
Regulation System ◽  
Instrumental Method ◽  
Hemostasis System ◽  
Hemostasis Factors

In article to discuss diagnostic value of blood aggregate regulation system (BARS) and complexity of diagnostics, particularly, in critical state.Advantages and disadvantages of instrumental diagnostics technique were analyzed: thromboelastographia and lowfrequency piezoelectric hemocoagulographia of whole blood. Estimates new next-generation instrumental method of research - lowfrequency contact conductometry. This method permits to evaluate role blood plasma, cells hemostasis factors and wall of vessels in critical state BARS dysfunction.

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