Ultrasensitive immunochromatographic strip assay for the detection of diminazene

The Analyst ◽  
2021 ◽  
Author(s):  
Mengjia chao ◽  
Xin-Xin Xu ◽  
Liqiang Liu ◽  
aihong wu ◽  
Shanshan Song ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Gold Nanoparticle ◽  
Immunosorbent Assay ◽  
Lateral Flow ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip ◽  
Flow Test ◽  
Lateral Flow Test

We prepared monoclonal antibodies (mAbs) against diminazene and used them in the development of a gold nanoparticle-based lateral-flow test (GNT) strip and indirect competitive enzyme-linked immunosorbent assay for the detection...

scholarly journals Wax-Assisted One-Step Enzyme-Linked Immunosorbent Assay on Lateral Flow Test Devices

2018 ◽  
Vol 34 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Masanori ISHII ◽  
Pattarachaya PREECHAKASEDKIT ◽  
Kentaro YAMADA ◽  
Orawon CHAILAPAKUL ◽  
Koji SUZUKI ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Lateral Flow ◽  
Enzyme Linked Immunosorbent Assay ◽  
Flow Test ◽  
One Step ◽  
Lateral Flow Test

Development of a monoclonal antibody for the detection of xylazine in milk and its use in an immunochromatographic strip

2021 ◽  
Author(s):  
Mengjia Chao ◽  
Liqiang Liu ◽  
Aihong Wu ◽  
Shanshan Song ◽  
Xinxin Xu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Gold Nanoparticle ◽  
Limit Of Detection ◽  
Lateral Flow ◽  
Immunochromatographic Strip ◽  
Naked Eye ◽  
Milk Samples ◽  
Flow Test ◽  
Lateral Flow Test

A gold nanoparticle-based lateral-flow test (GNT) strip was developed to detect xylazine (XYL) in milk. And the limit of detection (LOD) and cut-off value of the GNT assay were evaluated to be 20 and 200 ng mL−1 in milk samples by the naked eye.

scholarly journals Development of a Sensitive Enzyme-Linked Immunosorbent Assay and Rapid Gold Nanoparticle Immunochromatographic Strip for Detecting Citrinin in Monascus Fermented Food

Toxins ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 354 ◽  
Author(s):  
Shih-Wei Wu ◽  
Yao-An Yu ◽  
Biing-Hui Liu ◽  
Feng-Yih Yu
Keyword(s):  
Detection Limit ◽  
Gold Nanoparticle ◽  
Immunosorbent Assay ◽  
Keyhole Limpet Hemocyanin ◽  
Fermented Food ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fermented Foods ◽  
Close Inspection ◽  
Immunochromatographic Strip ◽  
Rapid Antibody

Antibodies against citrinin (CTN) were generated from rabbits, which were injected with CTN-keyhole limpet hemocyanin (KLH). This work involved the development of a sensitive competitive direct enzyme-linked immunosorbent assay (cdELISA) and a rapid gold nanoparticle immunochromatographic strip (immunostrip) method for analyzing CTN in Monascus-fermented food. CTN at a concentration of 5.0 ng/mL caused 50% inhibition (IC50) of CTN-horseradish peroxidase (CTN-HRP) binding to the antibodies in the cdELISA. The capable on-site detection of CTN was accomplished by a rapid antibody-gold nanoparticle immunostrip with a detection limit of 20 ng/mL and that was completed within 15 min. A close inspection of 19 Monascus-fermented foods by cdELISA confirmed that 14 were contaminated with citrinin at levels from 28.6–9454 ng/g. Further analysis with the immunostrip is consistent with those results obtained using cdELISA. Both means are sensitive enough for the rapid examination of CTN in Monascus-fermented food products.

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