Development of a monoclonal antibody for the detection of xylazine in milk and its use in an immunochromatographic strip

2021 ◽  
Author(s):  
Mengjia Chao ◽  
Liqiang Liu ◽  
Aihong Wu ◽  
Shanshan Song ◽  
Xinxin Xu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Gold Nanoparticle ◽  
Limit Of Detection ◽  
Lateral Flow ◽  
Immunochromatographic Strip ◽  
Naked Eye ◽  
Milk Samples ◽  
Flow Test ◽  
Lateral Flow Test

A gold nanoparticle-based lateral-flow test (GNT) strip was developed to detect xylazine (XYL) in milk. And the limit of detection (LOD) and cut-off value of the GNT assay were evaluated to be 20 and 200 ng mL−1 in milk samples by the naked eye.

Ultrasensitive immunochromatographic strip assay for the detection of diminazene

The Analyst ◽  
2021 ◽  
Author(s):  
Mengjia chao ◽  
Xin-Xin Xu ◽  
Liqiang Liu ◽  
aihong wu ◽  
Shanshan Song ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Gold Nanoparticle ◽  
Immunosorbent Assay ◽  
Lateral Flow ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip ◽  
Flow Test ◽  
Lateral Flow Test

We prepared monoclonal antibodies (mAbs) against diminazene and used them in the development of a gold nanoparticle-based lateral-flow test (GNT) strip and indirect competitive enzyme-linked immunosorbent assay for the detection...

scholarly journals Comparative evaluation of rapid isothermal amplification and antigen assays for screening testing of SARS-CoV-2

2021 ◽  
Author(s):  
Nol Salcedo ◽  
Brena F Sena ◽  
Xiying Qu ◽  
Bobby Brooke Herrera
Keyword(s):  
High Throughput Screening ◽  
Limit Of Detection ◽  
Low Cost ◽  
Lateral Flow ◽  
Isothermal Amplification ◽  
Clinical Samples ◽  
Peak Period ◽  
Percent Agreement ◽  
Flow Test ◽  
Lateral Flow Test

Human transmission of SARS-CoV-2 and emergent variants of concern has continued to occur globally, despite mass vaccination campaigns. Public health strategies to reduce virus spread should therefore rely, in part, on frequent screening with rapid, inexpensive, and sensitive tests. We evaluated two digitally integrated rapid tests and assessed their performance using stored nasal swab specimens collected from individuals with or without COVID-19. An isothermal amplification assay combined with a lateral flow test had a limit of detection of 10 RNA copies per reaction, and a positive percent agreement (PPA)/negative percent agreement (NPA) during the asymptomatic and symptomatic phases of 100%/100% and 95.83/100%, respectively. Comparatively, an antigen-based lateral flow test, had a limit of detection of 30,000 copies, and a PPA/NPA during the asymptomatic and symptomatic phases of 82.86%/98.68% and 91.67/100%, respectively. Both the isothermal amplification and antigen-based lateral flow tests had optimized detection of SARS-CoV-2 during the peak period of transmission; however, the antigen-based test had reduced sensitivity in clinical samples with qPCR Ct values greater than 29.8. Low-cost, high-throughput screening enabled by isothermal amplification or antigen-based techniques have value for outbreak control.

Validation of a Rapid Lateral Flow Test for the Simultaneous Determination of β-Lactam Drugs and Flunixin in Raw Milk

2012 ◽  
Vol 75 (7) ◽  
pp. 1270-1277 ◽  
Author(s):  
DAVID DOUGLAS ◽  
KATIE BANASZEWSKI ◽  
RIMA JUSKELIS ◽  
FADWA AL-TAHER ◽  
YANG CHEN ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Limit Of Detection ◽  
Agricultural Practices ◽  
Raw Milk ◽  
Lateral Flow ◽  
Penicillin G ◽  
Screening Programs ◽  
Flow Test ◽  
Lateral Flow Test

β-Lactam antibiotics are the most commonly used drugs on dairy farms. β-Lactam residues in milk are kept out of the human milk supply with good agricultural practices and mandatory truck screening performed by the dairy industry under Appendix N of the Pasteurized Milk Ordinance. Flunixin, a nonsteroidal and anti-inflammatory drug, appears in dairy cattle tissue residues with a frequency similar to the occurrence of penicillin G. This creates concern that flunixin residues could be in milk and would go undetected under current milk screening programs. A single test that combines mandatory β-lactam screening with voluntary flunixin screening is an economical approach for monitoring and controlling for potential flunixin or 5-hydroxyflunixin, the primary flunixin metabolite marker in milk. The objective of this study was to validate a β-lactam and flunixin rapid lateral flow test (LFT) and compare the results obtained with a liquid chromatography-triple quadrupole tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of flunixin and 5-hydroxyflunixin in raw milk with a limit of detection of <1 ppb, equivalent to 1 ng/ml. Using the LFT, three combined manufactured lots of test strips detected penicillin G at 2.0 ppb, ampicillin at 6.8 ppb, amoxicillin at 5.9 ppb, cephapirin at 13.4 ppb, ceftiofur (total metabolites) at 63 ppb, and 5-hydroxyflunixin at 1.9 ppb at least 90% of the time with 95% confidence. The LFT also detected incurred flunixin milk samples that were analyzed with the LC-MS/MS and diluted to tolerance in raw milk. The detection levels for the LFT are lower than the U.S. safe levels or tolerances and qualify the test to be used in compliance with U.S. milk screening programs.

A Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Treponema pallidum subsp. pertenue

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S128-S128
Author(s):  
Laud Anthony Basing
Keyword(s):  
Gold Standard ◽  
Limit Of Detection ◽  
Treponema Pallidum ◽  
Lamp Assay ◽  
Test Strip ◽  
Lateral Flow ◽  
Isothermal Amplification ◽  
Clinical Samples ◽  
Flow Test ◽  
Lateral Flow Test

Abstract Objectives The eradication of yaws, a childhood disease caused by Treponema pallidum subsp. pertenue, is constrained by the lack of rapid, accurate diagnosis. We sought to develop a molecular point-of-care test for the diagnosis of yaws. Methods A loop-mediated isothermal amplification (LAMP) assay with primers targeting the conserved gene, tp0967, with visual detection by lateral flow test strip was developed. The assay was optimized by varying the concentrations of reagents as well as the temperature and time of the reaction. The limit of detection and selectivity of the assay were evaluated. Subsequently, 63 clinical samples from yaws-infected lesions were used to determine the sensitivity of the assay from both unextracted and DNA extracted swab samples as compared to the current molecular testing protocol (CDC PCR assay) as the gold standard. A further five clinical samples from lesions containing PCR-confirmed syphilis pathogen (Treponema pallidum subsp. pallidum) were tested to ensure specificity of the assay for yaws alone. Results The developed LAMP assay was found to be optimal when run at 65oC for 30 minutes. The limit of detection was 2.7*104 copies DNA per mL. Out of the 63 yaws samples tested, the CDC assay, extracted DNA LAMP assay, and unextracted DNA using the LAMP assay resulted in 12, 14, and 8 positive results, respectively. None of the syphilis samples tested positive in any of the assays. Conclusion We show the development of a fast and sensitive LAMP assay for Treponema pallidum subsp. pertenue detected by lateral flow test strip. Using extracted DNA, the assay sensitivity is on par with gold standard detection. Further, the assay can be adapted to minimal sample processing required for in-field detection without DNA extraction.

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