Low-cost laser-cut patterned chips for acoustic concentration of micro to nano particles and cells by operating over a wide frequency range

The Analyst ◽  
2021 ◽  
Author(s):  
Jingui Qian ◽  
Wei Huang ◽  
Renhua Yang ◽  
Raymond H. W. Lam ◽  
Joshua Lee
Keyword(s):  
Acoustic Field ◽  
Particle Concentration ◽  
Low Cost ◽  
Wide Frequency Range ◽  
Nano Particles ◽  
Cell Manipulation ◽  
Label Free ◽  
Frequency Range ◽  
Spatial Asymmetry

Acoustofluidic platforms for cell manipulation benefit from being contactless and label-free at potentially low cost. Particle concentration in a droplet relies on augmenting spatial asymmetry in the acoustic field, which...

scholarly journals LOW-COST, INTELLIGENT DATA ACQUISITION SYSTEM FOR QCM AND OTHER RESONATOR-BASED BIO- AND CHEMICAL SENSORS

2014 ◽  
pp. 9-17
Author(s):  
Sergey Y. Yurish
Keyword(s):  
Data Acquisition ◽  
Integrated Circuit ◽  
Low Cost ◽  
Data Acquisition System ◽  
Wide Frequency Range ◽  
Acquisition System ◽  
Bulk Acoustic Wave ◽  
Frequency Range ◽  
Modified Method ◽  
Intelligent Data Acquisition

A low-cost, intelligent data acquisition system for quartz crystal microbalance (QCM) and other (superficial SAW and bulk acoustic wave) resonator-based chemical and biosensors described in this article. The system is based on the novel modified method of the dependent count and allows obtaining high metrological performances: programmable and constant in all frequency range relative error of measurement up to 0,0005 % (5 ppm); nonredundant, reduced conversion time of measurement and wide frequency range from 0.05 Hz to 9 MHz (144 MHz with prescaling). The developed system allows measuring variations of the resonance frequency shift for QCM sensors working in liquids and gases. The described low-cost data acquisition system is based on novel integrated circuit of universal frequency-to-digital converter. This design approach has eliminated much of the hardware: there is no need for a microcontroller and complex sensor interfacing electronics, as it is directly compatible with PC computers.

scholarly journals Gel-Like Human Mimicking Phantoms: Realization Procedure, Dielectric Characterization and Experimental Validations on Microwave Wearable Body Sensors

Biosensors ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 111
Author(s):  
Sandra Costanzo ◽  
Vincenzo Cioffi ◽  
Adil Masoud Qureshi ◽  
Antonio Borgia
Keyword(s):  
Low Cost ◽  
Wide Frequency Range ◽  
Frequency Range ◽  
Dielectric Parameters ◽  
Perfect Agreement ◽  
Dielectric Characterization ◽  
Human Arm ◽  
Body Sensors ◽  
Experimental Validations ◽  
5 Ghz

A simple and low-cost procedure for gel-like time-durable biological phantoms is presented in this work. Easily accessible materials are adopted, which are able to provide a flexible and controllable method to rapidly realize different kind of tissues. The proposed technique is applied to fabricate various tissue-mimicking phantoms, namely skin, muscle, blood and fat. Their effectiveness is first tested by performing dielectric characterization on a wide frequency range, from 500 MHz up to 5 GHz, and validating the measured dielectric parameters (dielectric constant and conductivity) by comparison with reference models in the literature. Then, a multi-layer phantom simulating the human arm is realized, and a wearable body sensor is adopted to prove the perfect agreement of the biometric response achieved in the presence of the fabricated phantom and that provided by a real human arm.

scholarly journals Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein

Biosensors ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 4
Author(s):  
Donggee Rho ◽  
Seunghyun Kim
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Optical Cavity ◽  
Sample Volume ◽  
Small Sample ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Cavity Structure

An optical cavity-based biosensor (OCB) has been developed for point-of-care (POC) applications. This label-free biosensor employs low-cost components and simple fabrication processes to lower the overall cost while achieving high sensitivity using a differential detection method. To experimentally demonstrate its limit of detection (LOD), we conducted biosensing experiments with streptavidin and C-reactive protein (CRP). The optical cavity structure was optimized further for better sensitivity and easier fluid control. We utilized the polymer swelling property to fine-tune the optical cavity width, which significantly improved the success rate to produce measurable samples. Four different concentrations of streptavidin were tested in triplicate, and the LOD of the OCB was determined to be 1.35 nM. The OCB also successfully detected three different concentrations of human CRP using biotinylated CRP antibody. The LOD for CRP detection was 377 pM. All measurements were done using a small sample volume of 15 µL within 30 min. By reducing the sensing area, improving the functionalization and passivation processes, and increasing the sample volume, the LOD of the OCB are estimated to be reduced further to the femto-molar range. Overall, the demonstrated capability of the OCB in the present work shows great potential to be used as a promising POC biosensor.

scholarly journals Integrating high-performing electrochemical transducers in lateral flow assay

2021 ◽  
Author(s):  
Antonia Perju ◽  
Nongnoot Wongkaew
Keyword(s):  
High Performance ◽  
Point Of Care ◽  
Low Cost ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Analytical Performance ◽  
Label Free ◽  
High Performing ◽  
Lateral Flow Assays ◽  
Electrochemical Transducers

AbstractLateral flow assays (LFAs) are the best-performing and best-known point-of-care tests worldwide. Over the last decade, they have experienced an increasing interest by researchers towards improving their analytical performance while maintaining their robust assay platform. Commercially, visual and optical detection strategies dominate, but it is especially the research on integrating electrochemical (EC) approaches that may have a chance to significantly improve an LFA’s performance that is needed in order to detect analytes reliably at lower concentrations than currently possible. In fact, EC-LFAs offer advantages in terms of quantitative determination, low-cost, high sensitivity, and even simple, label-free strategies. Here, the various configurations of EC-LFAs published are summarized and critically evaluated. In short, most of them rely on applying conventional transducers, e.g., screen-printed electrode, to ensure reliability of the assay, and additional advances are afforded by the beneficial features of nanomaterials. It is predicted that these will be further implemented in EC-LFAs as high-performance transducers. Considering the low cost of point-of-care devices, it becomes even more important to also identify strategies that efficiently integrate nanomaterials into EC-LFAs in a high-throughput manner while maintaining their favorable analytical performance.

scholarly journals Modifications of the PAMONO-Sensor Help to Size and Quantify Low Number of Individual Biological and Non-Biological Nano-Particles

2021 ◽  
Vol 6 (1) ◽  
pp. 26
Author(s):  
Rahat Morad Talukder ◽  
Al Shahriar Hossain Rakib ◽  
Julija Skolnik ◽  
Zohair Usfoor ◽  
Katharina Kaufmann ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Nano Particles ◽  
Label Free ◽  
Specific Detection ◽  
Virus Like Particles ◽  
Low Concentrations ◽  
Challenging Tasks ◽  
Image Area

In a series of recently published works, we demonstrated that the plasmon-assisted microscopy of nano-objects (PAMONO) technique can be successfully employed for the sizing and quantification of single viruses, virus-like particles, microvesicles and charged non-biological particles. This approach enables label-free, but specific detection of biological nano-vesicles. Hence, the sensor, which was built up utilizing plasmon-assisted microscopy, possesses relative versatility and it can be used as a platform for cell-based assays. However, one of the challenging tasks for such a sensor was the ability to reach a homogeneous illumination of the whole surface of the gold sensor slide. Moreover, in order to enable the detection of even relatively low concentrations of nano-particles, the focused image area had to be expanded. Both tasks were solved via modifications of previously described PAMONO-sensor set ups. Taken together, our latest findings can help to develop a research and diagnostic platform based on the principles of the surface plasmon resonance (SPR)-assisted microscopy of nano-objects.

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