A hairpin probe-mediated DNA circuit for the detection of mecA gene of Staphylococcus aureus based on exonuclease III and DNAzyme-mediated signal amplification

The Analyst ◽  
2021 ◽  
Author(s):  
Jiafeng Pan ◽  
Dongqin Bao ◽  
Enhu Bao ◽  
Junhua Chen
Keyword(s):  
Staphylococcus Aureus ◽  
Human Health ◽  
Signal Amplification ◽  
Meca Gene ◽  
Health And Safety ◽  
Exonuclease Iii ◽  
Hairpin Probe ◽  
Highly Sensitive

A simple, highly sensitive biosensor for S. aureus detection is becoming increasingly important in human health and safety. In this work, a hairpin probe-mediated DNA circuit for the detection of...

Ultrasensitive and simple fluorescence biosensor for detection of the mecA gene of Staphylococcus aureus by using an exonuclease III-assisted cascade signal amplification strategy

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5670-5675 ◽  
Author(s):  
Qiong Li ◽  
Danhua Zhou ◽  
Jiafeng Pan ◽  
Zhi Liu ◽  
Junhua Chen
Keyword(s):  
Staphylococcus Aureus ◽  
Signal Amplification ◽  
Meca Gene ◽  
Exonuclease Iii ◽  
G Quadruplex ◽  
Exo Iii ◽  
Fluorescence Biosensor ◽  
Amplification Strategy

An ultrasensitive biosensor was developed for the detection of the mecA gene of Staphylococcus aureus based on the Exo III and G-quadruplex.

A highly sensitive fluorescence biosensor for detection of Staphylococcus aureus based on HCR-mediated three-way DNA junction nicking enzyme assisted signal amplification

The Analyst ◽  
2021 ◽  
Author(s):  
Chuyan Zhang ◽  
Zewei Luo ◽  
Mengfan Wu ◽  
Wei Ning ◽  
Ziyi Tian ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Food Safety ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Safety Control ◽  
Food Borne ◽  
Highly Sensitive ◽  
Fluorescence Biosensor ◽  
Highly Sensitive Detection ◽  
Nicking Enzyme

Sensitive and efficient monitoring of food-borne bacteria is of great importance for food safety control. Herein, a novel biosensor for highly sensitive detection of Staphylococcus aureus (S. aureus) was constructed...

scholarly journals Occurrence of Methicillin-Resistant Coagulase-Negative Staphylococci (MRCoNS) and Methicillin-Resistant Staphylococcus aureus (MRSA) from Pigs and Farm Environment in Northwestern Italy

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 676
Author(s):  
Miryam Bonvegna ◽  
Elena Grego ◽  
Bruno Sona ◽  
Maria Cristina Stella ◽  
Patrizia Nebbia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Human Health ◽  
Environmental Samples ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bacterial Species ◽  
Meca Gene ◽  
Nucleotide Sequencing ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Nasal Swabs

Swine farming as a source of methicillin-resistant Staphylococcus aureus (MRSA) has been well documented. Methicillin-resistant coagulase-negative staphylococci (MRCoNS) have been less studied, but their importance as pathogens is increasing. MRCoNS are indeed considered relevant nosocomial pathogens; identifying putative sources of MRCoNS is thus gaining importance to prevent human health hazards. In the present study, we investigated MRSA and MRCoNS in animals and environment in five pigsties in a high farm-density area of northwestern Italy. Farms were three intensive, one intensive with antibiotic-free finishing, and one organic. We tested nasal swabs from 195 animals and 26 environmental samples from three production phases: post-weaning, finishing and female breeders. Phenotypic tests, including MALDI-TOF MS, were used for the identification of Staphylococcus species; PCR and nucleotide sequencing confirmed resistance and bacterial species. MRCoNS were recovered in 64.5% of nasal swabs, in all farms and animal categories, while MRSA was detected only in one post-weaning sample in one farm. The lowest prevalence of MRCoNS was detected in pigs from the organic farm and in the finishing of the antibiotic-free farm. MRCoNS were mainly Staphylococcus sciuri, but we also recovered S. pasteuri, S. haemolyticus, S. cohnii, S. equorum and S. xylosus. Fifteen environmental samples were positive for MRCoNS, which were mainly S. sciuri; no MRSA was found in the farms’ environment. The analyses of the mecA gene and the PBP2-a protein highlighted the same mecA fragment in strains of S. aureus, S. sciuri and S. haemolyticus. Our results show the emergence of MRCoNS carrying the mecA gene in swine farms. Moreover, they suggest that this gene might be horizontally transferred from MRCoNS to bacterial species more relevant for human health, such as S. aureus.

Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4267-4272 ◽  
Author(s):  
Biqing Bao ◽  
Yanrui Pan ◽  
Bingbing Gu ◽  
Jia Chen ◽  
Yu Xu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Conjugated Polyelectrolytes ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Exo Iii ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Cascade Amplification

A ratiometric and cascade amplification strategy that combines the signal amplification and effecitive FRET property of CPEs with the Exo III-assisted target recycling method has been developed for DNA detection.

Gold nanoparticle-based exonuclease III signal amplification for highly sensitive colorimetric detection of folate receptor

Nanoscale ◽  
2014 ◽  
Vol 6 (6) ◽  
pp. 3055-3058 ◽  
Author(s):  
Xinjian Yang ◽  
Zhiqiang Gao
Keyword(s):  
Gold Nanoparticles ◽  
Small Molecule ◽  
Signal Amplification ◽  
Colorimetric Assay ◽  
Folate Receptor ◽  
Colorimetric Detection ◽  
Label Free ◽  
Exonuclease Iii ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

By combining terminal protection of small molecule (folate)-capped DNA probes, exonuclease III signal amplification and gold nanoparticles, we developed a simple and label-free colorimetric assay for highly sensitive detection of folate receptor.

Highly sensitive DNA-based fluorometric mercury(II) bioassay based on graphene oxide and exonuclease III-assisted signal amplification

2015 ◽  
Vol 182 (7-8) ◽  
pp. 1535-1541 ◽  
Author(s):  
Yulin Zhang ◽  
Lina Tang ◽  
Fan Yang ◽  
Zhongyue Sun ◽  
Guo-Jun Zhang
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Exonuclease Iii ◽  
Highly Sensitive

Label-free and highly sensitive fluorescence detection of lead(ii) based on DNAzyme and exonuclease III-assisted cascade signal amplification

2019 ◽  
Vol 43 (15) ◽  
pp. 5857-5862 ◽  
Author(s):  
Jiafeng Pan ◽  
Qiong Li ◽  
Danhua Zhou ◽  
Junhua Chen
Keyword(s):  
Fluorescence Detection ◽  
Signal Amplification ◽  
Label Free ◽  
Exonuclease Iii ◽  
Highly Sensitive ◽  
Exo Iii

A Pb2+ biosensor has been constructed based on Exo III-assisted cascade signal amplification using 2-amino-5,6,7-trimethyl-1,8-naphthyridine as the signal indicator.

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