A PLGA nanofiber microfluidic device for highly efficient isolation and release of different phenotypic circulating tumor cells based on dual aptamers

2021 ◽  
Author(s):  
Zeen Wu ◽  
Yue Pan ◽  
Zhili Wang ◽  
Pi Ding ◽  
Tian Gao ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Device ◽  
Microfluidic Devices ◽  
Highly Efficient

Dual aptamer-modified PLGA nanofiber-based microfluidic devices were fabricated to achieve the highly efficient isolation and specific release of epithelial and mesenchymal CTCs.

scholarly journals Highly efficient capture of circulating tumor cells by microarray in a microfluidic device

2019 ◽  
Vol 33 (S1) ◽  
Author(s):  
Hui‐Yu Liu ◽  
Claudia Hille ◽  
Anna Haller ◽  
Ravi Kumar ◽  
Klaus Pantel ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Device ◽  
Highly Efficient

Interactions Between Circulating Tumor Cells and Aptamer-Functionalized Microposts in a Flow

2017 ◽  
Author(s):  
Kangfu Chen ◽  
Teodor Georgiev ◽  
Z. Hugh Fan
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Device ◽  
Blood Cells ◽  
Volume Ratio ◽  
Microfluidic Devices ◽  
Cancer Prognosis ◽  
Device Performance ◽  
Simulation Methodology ◽  
Geometry Design

Circulating Tumor Cells (CTCs) have been considered as important biomarkers for cancer prognosis and treatment. However, there are only tens of CTCs in one billion of healthy blood cells. This CTC rarity challenge has been addressed by microfluidics technology that sheds light on efficient CTC detection and isolation. Using antibodies or aptamers to capture CTCs is one of the strategies for CTC isolation. A lot of work has been carried out to improve CTC capture efficiency and purity (i.e., specificity). The main consideration to optimize microfluidic device performance includes increasing surface-area-to-volume ratio and reducing shear stress, both of which are closely related to the interaction between CTCs and the microfluidic device. Here we report a detailed study on the interactions between CTCs and aptamer-functionalized microposts in a microfluidic device. We have evaluated the distribution of captured CTCs around a micropost. In addition, simulation was conducted to model CTC capture patterns around microposts. We found the simulated CTC capture pattern largely agree with the experimental results. The simulation methodology could be applicable for other affinity-based CTC isolation devices and approaches. The goal of the study is to improve the microfluidic device performance and provide a rapid and economical way to optimize the geometry design of the microfluidic devices for CTC isolation.

scholarly journals Numerical Simulation of Separation of Circulating Tumor Cells from Blood Stream in Deterministic Lateral Displacement (DLD) Microfluidic Channel

2015 ◽  
Vol 32 (4) ◽  
pp. 463-471 ◽  
Author(s):  
F. Khodaee ◽  
S. Movahed ◽  
N. Fatouraee ◽  
F. Daneshmand
Keyword(s):  
Fluid Flow ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Device ◽  
Flow Condition ◽  
Lateral Displacement ◽  
Microfluidic Devices ◽  
Blood Stream ◽  
Deterministic Lateral Displacement ◽  
Effective Design

AbstractDeterministic Lateral Displacement (DLD) microfluidic devices provide a reliable label-free separation method for detection of circulating tumor cells (CTCs) in blood samples based on their biophysical properties. In this paper, we proposed an effective design of the DLD microfluidic device for the CTC separation in the blood stream. A typical DLD array is designed and numerical simulations are performed to separate the CTC and leukocyte (white blood cells) in different fluid flow conditions. Fluid-Solid Interaction method is used to investigate the behaviour of these deformable cells in fluid flow. In this study, the effects of critical parameters affecting cell separation in the DLD microfluidic devices (e.g.flow condition, cell deformability, and stress) have been investigated. The obtained results show that unlike leukocytes, the CTC’s motion is independent of the flow condition and is laterally displaced even in higher Reynolds number. Larger cells (CTCs) cannot intercept the low-velocity fluid near the wall of the posts; thus, they move faster and become separated from leukocytes. To reduce the cellular stress during separation process, which causes increase of cell viability and more effective design of microfluidic device, the results obtained here may be used as a significant design parameter for the DLD fabrication.

Highly Efficient Isolation of Circulating Tumor Cells Using a Simple Wedge-Shaped Microfluidic Device

2019 ◽  
Vol 66 (6) ◽  
pp. 1536-1541 ◽  
Author(s):  
Luman Qin ◽  
Wei Zhou ◽  
Shoukun Zhang ◽  
Boran Cheng ◽  
Shubin Wang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Device ◽  
Highly Efficient

scholarly journals Aptamer-Conjugated Graphene Oxide Membranes for Highly Efficient Capture and Accurate Identification of Multiple Types of Circulating Tumor Cells

2015 ◽  
Vol 26 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Bhanu Priya Viraka Nellore ◽  
Rajashekhar Kanchanapally ◽  
Avijit Pramanik ◽  
Sudarson Sekhar Sinha ◽  
Suhash Reddy Chavva ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Accurate Identification ◽  
Highly Efficient ◽  
Oxide Membranes ◽  
Graphene Oxide Membranes

Integration of aligned polymer nanofibers within a microfluidic chip for efficient capture and rapid release of circulating tumor cells

2018 ◽  
Vol 2 (5) ◽  
pp. 891-900 ◽  
Author(s):  
Yunchao Xiao ◽  
Mengyuan Wang ◽  
Lizhou Lin ◽  
Lianfang Du ◽  
Mingwu Shen ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Chip ◽  
Polymer Nanofibers ◽  
Highly Efficient ◽  
Rapid Release ◽  
Aligned Nanofibers

Zwitterion-functionalized aligned nanofibers integrated with a microfluidic chip can be used for highly efficient capture and rapid release of CTCs.

scholarly journals Hybrid negative enrichment of circulating tumor cells from whole blood in a 3D-printed monolithic device

Lab on a Chip ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 3427-3437 ◽  
Author(s):  
Chia-Heng Chu ◽  
Ruxiu Liu ◽  
Tevhide Ozkaya-Ahmadov ◽  
Mert Boya ◽  
Brandi E. Swain ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Device ◽  
Whole Blood ◽  
3D Printed

A monolithic 3D-printed microfluidic device integrated with stacked layers of functionalized leukodepletion channels and microfiltration for the negative enrichment of circulating tumor cells directly from clinically relevant volumes of whole blood.

scholarly journals Cancer Diagnostics: Visual Quantitative Detection of Circulating Tumor Cells with Single‐Cell Sensitivity Using a Portable Microfluidic Device (Small 14/2019)

Small ◽  
2019 ◽  
Vol 15 (14) ◽  
pp. 1970075
Author(s):  
Mahlet Fasil Abate ◽  
Shasha Jia ◽  
Metages Gashaw Ahmed ◽  
Xingrui Li ◽  
Li Lin ◽  
...  
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Device ◽  
Cancer Diagnostics ◽  
Quantitative Detection ◽  
Cell Sensitivity

scholarly journals Nanotechnology-Assisted Isolation and Analysis of Circulating Tumor Cells on Microfluidic Devices

Micromachines ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 774 ◽  
Author(s):  
Jie Cheng ◽  
Yang Liu ◽  
Yang Zhao ◽  
Lina Zhang ◽  
Lingqian Zhang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Liquid Biopsy ◽  
Cancer Metastasis ◽  
Human Peripheral Blood ◽  
Microfluidic Devices ◽  
Detection Sensitivity ◽  
Cell Capture ◽  
Primary Tumors ◽  
Downstream Analysis

Circulating tumor cells (CTCs), a type of cancer cell that spreads from primary tumors into human peripheral blood and are considered as a new biomarker of cancer liquid biopsy. It provides the direction for understanding the biology of cancer metastasis and progression. Isolation and analysis of CTCs offer the possibility for early cancer detection and dynamic prognosis monitoring. The extremely low quantity and high heterogeneity of CTCs are the major challenges for the application of CTCs in liquid biopsy. There have been significant research endeavors to develop efficient and reliable approaches to CTC isolation and analysis in the past few decades. With the advancement of microfabrication and nanomaterials, a variety of approaches have now emerged for CTC isolation and analysis on microfluidic platforms combined with nanotechnology. These new approaches show advantages in terms of cell capture efficiency, purity, detection sensitivity and specificity. This review focuses on recent progress in the field of nanotechnology-assisted microfluidics for CTC isolation and detection. Firstly, CTC isolation approaches using nanomaterial-based microfluidic devices are summarized and discussed. The different strategies for CTC release from the devices are specifically outlined. In addition, existing nanotechnology-assisted methods for CTC downstream analysis are summarized. Some perspectives are discussed on the challenges of current methods for CTC studies and promising research directions.

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