Evidence of protein coronas around soft nanoparticles regardless of the chemical nature of the outer surface: structural features and biological consequences

2021 ◽  
Author(s):  
Carlos E. de Castro ◽  
Karine Panico ◽  
Lucas M. Stangherlin ◽  
Lindomar J. C. Albuquerque ◽  
Caroline A. S. Ribeiro ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Outer Surface ◽  
Chemical Nature ◽  
Structural Features ◽  
Ph Responsive ◽  
Cytotoxic Effects ◽  
Polymeric Assemblies ◽  
Soft Nanoparticles

The presence of thick protein coronas has been found around pH-responsive polymeric assemblies. They reduce the levels of cellular uptake, nevertheless with positive outputs with regard to cytotoxic effects.

Cytotoxic Effect of 5-Fluorouracil-loaded Polymer-coated Magnetite Nanographene Oxide Combined with Radiofrequency

2018 ◽  
Vol 18 (8) ◽  
pp. 1148-1155 ◽  
Author(s):  
Leili Asadi ◽  
Sakine Shirvalilou ◽  
Sepideh Khoee ◽  
Samideh Khoei
Keyword(s):  
Cellular Uptake ◽  
Cancer Cell Line ◽  
Superparamagnetic Iron Oxide ◽  
Colon Cancer Cell Line ◽  
Cytotoxic Effects ◽  
Proliferation Capacity ◽  
Capacity Level ◽  
Colony Number ◽  
Nano Graphene ◽  
Dialysis Bag

Background: Despite the development of conventional therapies including surgery, radiotherapy, chemotherapy and hyperthermia, the prognosis remains very poor. Recently, integration of conventional therapy and multifunctional nanoparticles have attracted a lot of attention because it produces a synergistic effect and better diagnostic and therapeutic efficiency. Objective: This study aimed to investigate the uptake and cytotoxic effects of Polycaprolactone (PCL)/chitosan (CHI)-coated Superparamagnetic Iron Oxide Nano-Graphene Oxide (SPION-NGO) as a carrier of 5-fluorouracil (5-Fu) and Radiofrequency (RF) hyperthermia using an Alternate Magnetic Field (AMF) with 13.56 MHz frequency on the proliferation capacity level of CT26 colon cancer cell line in a monolayer culture. Method: The release of the newly synthesised 5-Fu-loaded PCL/CHI-SPION-NGO was measured in Phosphate Buffered Saline (PBS) using the dialysis bag method. The cellular uptake of 5-Fu-loaded PCL/CHI-SPIONNGO was measured using Atomic Absorption Spectroscopy (AAS). The cytotoxic effects of 5-Fu, 5-Fu- PCL/CHI-SPION-NGO and PCL/CHI-SPION-NGO with and without RF hyperthermia were determined using the colony formation assay. Results: Particle size and zeta potential of 5-Fu-PCL/CHI-SPION-NGO and PCL/CHI-SPION-NGO were 61.2 nm and -1.87 mV and 43.4 nm and -10.19 mV, respectively. Spectroscopy results demonstrated that the cellular uptake of 5-Fu-PCL/CHI-SPION-NGO increased with elevated nanostructure concentrations. The results revealed that the proliferation capacity of the cells decreased with 5-Fu or 5-Fu-PCL/CHI-SPION-NGO in combination with RF hyperthermia. Furthermore, extent of reduction in colony number following treatment with 5-Fu-PCL/CHI-SPION-NGO in combination with AMF was significantly more than 5-Fu + hyperthermia. Conclusion: Therefore, PCL/CHI-SPION-NGO can deliver 5-Fu more efficiently into the CT26 cells.

Dual pH-responsive micelles with both charge-conversional property and hydrophobic–hydrophilic transition for effective cellular uptake and intracellular drug release

2016 ◽  
Vol 7 (12) ◽  
pp. 2202-2208 ◽  
Author(s):  
Shuai Li ◽  
Zhouxiang Zhao ◽  
Wei Wu ◽  
Chunmei Ding ◽  
Jianshu Li
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Cellular Uptake ◽  
Diblock Copolymers ◽  
Effective Drug ◽  
Ph Responsive ◽  
A Charge

Two types of diblock copolymers are synthesized to construct dual pH-responsive micelles with a charge-conversional property for effective drug delivery.

Single Stranded Polydeoxyribonucleotides Compete with the Incorporation of [3H] Thymidine or [3H]dAMP into DNA.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4444-4444
Author(s):  
Ting Yang ◽  
Raymund Buhmann ◽  
Michaela Svihla ◽  
Hans-Jochem Kolb
Keyword(s):  
T Cells ◽  
Fluorescence Microscopy ◽  
Cellular Uptake ◽  
T Cell Activation ◽  
Cell Activation ◽  
Cellular Proliferation ◽  
Trypan Blue Exclusion ◽  
Activation Markers ◽  
Cytotoxic Effects ◽  
Current Survey

Abstract Defibrotide, the sodium salt of a single-stranded polydeoxyribonucleotide is prepared by a controlled depolymerisation of deoxyribonucleic acid (DNA) which is obtained from mammalian organs. In previous studies it was shown to mediate immunosuppressive effects (Ferraresso et al. 1993)1. In the current survey we investigated whether randomly chemically synthesized single-stranded polydeoxyribonucleotides of different length and composition could provide similar effects. For this purpose, purified T-cells were stimulated in the presence of dNTP’s or single-stranded polydeoxyribonucleotides with irradiated, allogeneic PBMC’s, PHA or anti-CD3/CD28 Dynabeads. Cellular proliferation was assessed by incorporation of tritium-labelled thymidine ([3H]thymidine), respectively [3H]dAMP or by staining with CFSE (carboxyfluorescein succinimidyl ester). After 72h or 120h of incubation, the incorporation of [3H]thymidine, or [3H]dAMP as well as the CFSE distribution was assessed. Cell viability was measured by trypan blue exclusion. T-cell activation was measured after 72h by quantifying the number of CD3+ T-cells expressing the activation markers CD25 and CD69. Cellular uptake of fluorochrome-labelled oligos was detected by fluorescence microscopy using an AxioCam HR and visualized by AxioVison. Each experiment was performed at least three times. Polydeoxyribonucleotides of different length, composition or concentrations (up to 5mM) did not cause cytotoxic effects to lymphocytes. But the incorporation of [3H]thymidine or [3H]dAMP was competed by polydeoxyribonucleotides. These effects were found to be dependent on length, concentration and base-composition of the nucleic acids. The proliferative capacity of the T-cells, as assessed by CFSE-staining, seemed to be unaffected. In context with data obtained by fluorescence microscopy, we hypothesise that there might be a cellular uptake of the polydeoxyribonucleotides followed by a subsequent cytoplasmatic degradation and a intracellular reutilisation via the salvage pathway for synthesis of nucleotides. The standard approach to detect cellular proliferation by incorporation of tritium-labelled nucleotides or derivatives is not useful to assess changes in cellular metabolism or proliferation in context with polydeoxyribonucleotides. Moreover, and that might be even more important, treatment approaches using nucleoside analogues like fludarabine, cytarabine e.g. in context with polydeoxyribonucleotides might be critical and diminish the efficacy of these drugs. Further experiments are necessary to elucidate the precise mechanism of these effects.

Synthesis of Gold Nanoparticles Coated with pH-Responsive Polymers and Evaluation of the Cellular Uptake

2012 ◽  
Vol 1416 ◽  
Author(s):  
Takeo Ito ◽  
Eriko Kusaka ◽  
Yu Isobe ◽  
Sei-ichi Nishimoto
Keyword(s):  
Cellular Uptake ◽  
Gold Nanorods ◽  
Near Infrared ◽  
A549 Cells ◽  
Endocytic Pathway ◽  
Human Lung Cancer ◽  
Ph Responsive ◽  
Absorption Bands ◽  
Responsive Polymers ◽  
Ph Responsive Polymers

ABSTRACTGold nanorods (AuNRs) show surface plasmon absorption bands in the near-infrared region. This characteristic property has stimulated utilization of gold nanorods as novel nanoprobes for noninvasive bioimaging, such as photoacoustic tomography. Herein, we discuss the synthesis of a series of gold nanorods coated with pH-responsive polymers to investigate the effect of the surface structure and zeta potential of nanoparticles on cellular uptake via a surface charge-mediated endocytic pathway. The surface of the gold nanorods was modified with polyethylene glycol (PEG@AuNR) and tertiary amine derivatives, specifically, diethylaminoethyl ester (1@AuNRs), its amide analog (2@AuNRs), and dimethylaminoethyl ester (3@AuNRs). It was found that the pH-sensitivity of1@AuNRs was relatively high and the surface was positively charged at lower pH. In contrast, the tertiary amino group of1@AuNRs was deprotonated to form an electrostatically neutral surface at higher pH. The pH-responsive gold nanorods were incubated with A549 cells (human lung cancer cells) to quantify the amount of cellular uptake using inductively coupled plasma mass spectrometry. The results indicate that1@AuNRs can be taken up efficiently in the cells, and thereafter, slowly flow out of the cells. Interestingly, only small amounts of the amide analog (2@AuNRs) were taken into the cells, suggesting minor structural changes may affect the interaction between the cell surface and AuNRs. This study highlights a potential application of pH-sensitive nanorods as a probe for bioimaging the acidic environment of tumors.

scholarly journals Surface-induced assembly of sophorolipids

2017 ◽  
Vol 19 (23) ◽  
pp. 15227-15238 ◽  
Author(s):  
Jessie Peyre ◽  
Ahmed Hamraoui ◽  
Marco Faustini ◽  
Vincent Humblot ◽  
Niki Baccile
Keyword(s):  
Self Assembly ◽  
Chemical Nature ◽  
Ph Responsive

The surface self-assembly properties of acidic sophorolipids, a bolaform microbial glycolipid with pH-responsive properties, were studied based on the chemical nature of the support and pH of the solution.

Preparation of PEG Functionalized Nanoparticles for Drug Delivery to Cancer Cells

2014 ◽  
Vol 893 ◽  
pp. 194-197
Author(s):  
Yan Liu ◽  
Jie Ren ◽  
Jun Zhao Leng ◽  
Jian Bo Li ◽  
Li Deng
Keyword(s):  
Drug Delivery ◽  
Polyethylene Glycol ◽  
Calcium Phosphate ◽  
Zeta Potential ◽  
Cellular Uptake ◽  
Physiological Solution ◽  
Ph Responsive ◽  
Functionalized Nanoparticles ◽  
Calcium Phosphate Nanoparticles ◽  
Reverse Microemulsion Method

Nanosized calcium phosphate was prepared by a reverse microemulsion method, with a 20~40 nm diameter, which is pH-responsive, nontoxic and colloidally stable in physiological solution. Polyethylene glycol modified calcium phosphate nanoparticles shifted the zeta potential to a neutral charge, which prolonged the nanoparticle circulation time and increased cellular uptake efficacy to targeted cells. The PEG-functionalized nanoparticles exhibit a great potential for efficaciously delivering hydrophobic anticancer drug, such as paclitaxel, to cells and tumors.

pH-responsive hydrogel cubes for release of doxorubicin in cancer cells

2014 ◽  
Vol 2 (17) ◽  
pp. 2494-2507 ◽  
Author(s):  
Veronika Kozlovskaya ◽  
Jun Chen ◽  
Chrysanty Tedjo ◽  
Xing Liang ◽  
Javier Campos-Gomez ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Methacrylic Acid ◽  
Ph Responsive

Doxorubicin (DOX)-loaded poly(methacrylic acid) hydrogel cubes release the drug at pH <5. These hydrogels are developed for shape-directed cellular uptake for drug delivery.

Collagen Structure and Its Modifications at Different Diseases

2016 ◽  
Vol 2 (11) ◽  
Author(s):  
Natalia Lisitza
Keyword(s):  
Extracellular Matrix ◽  
Wide Spectrum ◽  
Dominant Role ◽  
Therapy Response ◽  
Structural Features ◽  
Collagen Structure ◽  
Cellular Functions ◽  
Collagen Types ◽  
Abnormal Expression ◽  
Polymeric Assemblies

<p>The collagen superfamily of proteins plays a dominant role in maintaining the integrity of various tissues and also has a number of other important functions. The superfamily includes more than 20 collagen types with altogether. Most collagens form polymeric assemblies. such as fibrils, networks and filaments, and the superfamily can be divided into several families based on these assemblies and structural features. The critical roles of collagens have been clearly illustrated by the wide spectrum of diseases caused by the mutations that occur in genes coding different collagen types. Collagens are also major components of the extracellular matrix (ECM). The abnormal expression, proteolysis and structure of these collagens influence cellular functions to elicit multiple effects on tumors, including proliferation, initiation, invasion, metastasis, and therapy response.</p>

Cellular uptake of pH/reduction responsive phosphorylcholine micelles

2017 ◽  
Vol 41 (20) ◽  
pp. 11828-11838 ◽  
Author(s):  
Yuanyuan Cai ◽  
Shuai Li ◽  
Mengtan Cai ◽  
Yuanwei Chen ◽  
Xianglin Luo
Keyword(s):  
Drug Release ◽  
Cellular Uptake ◽  
Drug Carriers ◽  
Ph Responsive ◽  
Ph Reduction ◽  
The Relationship

We study the relationship between the PDEA content and internalization/intracellular drug release of pH responsive phosphorylcholine micelles as drug carriers.

scholarly journals Development and Evaluation of Multifunctional Poly(Lactic-co-glycolic acid) Nanoparticles Embedded in Carboxymethyl β-Glucan Porous Microcapsules as a Novel Drug Delivery System for Gefitinib

Pharmaceutics ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 469 ◽  
Author(s):  
Xiaonan Li ◽  
Jinglei Wang ◽  
Shang Li ◽  
Zhaorong Liu ◽  
Zhiru Zheng ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Targeted Delivery ◽  
Glycolic Acid ◽  
Lung Tumor ◽  
A549 Cells ◽  
Amorphous State ◽  
Poloxamer 407 ◽  
Hydrodynamic Diameter ◽  
Prolonged Release ◽  
Ph Responsive

In this study, a new kind of folic acid (FA)-conjugated and chitosan (CS)-coated poloxamer 407 (P407)/poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs), FCPP NPs, were prepared, and further micro-encapsulated by carboxymethyl β-glucan microcapsules (MCs) to produce a multifunctional system of NPs embedded in MCs (NEMs) for potential lung tumor-targeted delivery of gefitinib. The prepared gefitinib-loaded FCPP (GFB/FCPP) NPs showed a hydrodynamic diameter of 255.4 ± 14.5 nm and existed in an amorphous state. After encapsulation in carboxymethyl β-glucan MCs, the GFB/FCPP-based NEMs (GFB/FCPP-NEMs) also exhibited a spherical morphology with a median diameter (d50) of around 2.2 μm. After hydration, GFB/FCPP- NEMs can quickly dissociate into its primary particles, GFB/FCPP NPs. Our in vitro drug release study revealed that these GFB/FCPP-NEMs exhibited a pH-responsive prolonged release property. In addition, the cellular uptake study demonstrated that FCPP-NEMs serve as an efficient carrier to enhance the delivery of the entrapped drug into the target lung tumor cells. Moreover, the GFB/FCPP-NEMs induced a superior cytotoxic effect compared with free gefitinib. The inhibitory concentration to achieve 50% cell death (IC50) of GFB/FCPP-NEMs in A549 cells was 3.82-fold lower than that of free gefitinib. According to these results, FCPP-NEMs hold a great potential as a multifunctional and high-performance biomaterial for lung tumor targeting delivery, pH-responsive sustained release, facilitated cellular uptake, and enhanced antitumor effect of antitumor drugs, like gefitinib.

Sign in / Sign up

Export Citation Format

Share Document