Biomimetic magnetofluorescent ferritin nanoclusters for magnetic resonance and fluorescence-dual modal imaging and targeted tumor therapy

2021 ◽  
Author(s):  
Rongguang Song ◽  
Jian Dai Dai ◽  
Miaoliang Ruan ◽  
Wei Xue
Keyword(s):  
Magnetic Resonance ◽  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Tumor Therapy ◽  
Multiple Imaging

Multiple imaging by combing magnetic resonance (MR) and fluorescence imaging into a single nanosystem displays distinctive merits, which is desirable for precise in vivo imaging. In this work, we proposed...

scholarly journals Responsive Trimodal Probes for In Vivo Imaging of Liver Inflammation by Coassembly and GSH-Driven Disassembly

Research ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Yuxuan Hu ◽  
Yuqi Wang ◽  
Xidan Wen ◽  
Yifan Pan ◽  
Xiaoyang Cheng ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Ex Vivo ◽  
Molecular Probes ◽  
Liver Inflammation ◽  
Diagnosis And Prognosis ◽  
Imaging Probes ◽  
Substantial Decline

Noninvasive in vivo imaging of hepatic glutathione (GSH) levels is essential to early diagnosis and prognosis of acute hepatitis. Although GSH-responsive fluorescence imaging probes have been reported for evaluation of hepatitis conditions, the low penetration depth of light in liver tissue has impeded reliable GSH visualization in the human liver. We present a liver-targeted and GSH-responsive trimodal probe (GdNPs-Gal) for rapid evaluation of lipopolysaccharide- (LPS-) induced acute liver inflammation via noninvasive, real-time in vivo imaging of hepatic GSH depletion. GdNPs-Gal are formed by molecular coassembly of a GSH-responsive Gd(III)-based MRI probe (1-Gd) and a liver-targeted probe (1-Gal) at a mole ratio of 5/1 (1-Gd/1-Gal), which shows high r1 relaxivity with low fluorescence and fluorine magnetic resonance spectroscopic (19F-MRS) signals. Upon interaction with GSH, 1-Gd and 1-Gal are cleaved and GdNPs-Gal rapidly disassemble into small molecules 2-Gd, 2-Gal, and 3, producing a substantial decline in r1 relaxivity with compensatory enhancements in fluorescence and 19F-MRS. By combining in vivo magnetic resonance imaging (1H-MRI) with ex vivo fluorescence imaging and 19F-MRS analysis, GdNPs-Gal efficiently detect hepatic GSH using three independent modalities. We noninvasively visualized LPS-induced liver inflammation and longitudinally monitored its remediation in mice after treatment with an anti-inflammatory drug, dexamethasone (DEX). Findings highlight the potential of GdNPs-Gal for in vivo imaging of liver inflammation by integrating molecular coassembly with GSH-driven disassembly, which can be applied to other responsive molecular probes for improved in vivo imaging.

scholarly journals BIMG-08. DEUTERIUM MAGNETIC RESONANCE SPECTROSCOPY USING 2H-PYRUVATE ALLOWS NON-INVASIVE IN VIVO IMAGING OF TERT EXPRESSION IN BRAIN TUMORS

2021 ◽  
Vol 3 (Supplement_1) ◽  
pp. i2-i2
Author(s):  
Georgios Batsios ◽  
Celine Taglang ◽  
Meryssa Tran ◽  
Anne Marie Gillespie ◽  
Joseph Costello ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
In Vivo Imaging ◽  
Lactate Production ◽  
Telomere Maintenance ◽  
Resonance Spectroscopy ◽  
Low Grade ◽  
Non Invasive ◽  
Tert Expression

Abstract Telomere shortening constitutes a natural barrier to uncontrolled proliferation and all tumors must find a mechanism of maintaining telomere length. Most human tumors, including high-grade primary glioblastomas (GBMs) and low-grade oligodendrogliomas (LGOGs) achieve telomere maintenance via reactivation of the expression of telomerase reverse transcriptase (TERT), which is silenced in normal somatic cells. TERT expression is, therefore, a driver of tumor proliferation and, due to this essential role, TERT is also a therapeutic target. However, non-invasive methods of imaging TERT are lacking. The goal of this study was to identify magnetic resonance spectroscopy (MRS)-detectable metabolic biomarkers of TERT expression that will enable non-invasive visualization of tumor burden in LGOGs and GBMs. First, we silenced TERT expression by RNA interference in patient-derived LGOG (SF10417, BT88) and GBM (GS2) models. Our results linked TERT silencing to significant reductions in steady-state levels of NADH in all models. NADH is essential for the conversion of pyruvate to lactate, suggesting that measuring pyruvate flux to lactate could be useful for imaging TERT status. Recently, deuterium (2H)-MRS has emerged as a novel, clinically translatable method of monitoring metabolic fluxes in vivo. However, to date, studies have solely examined 2H-glucose and the use of [U-2H]pyruvate for non-invasive 2H-MRS has not been tested. Following intravenous injection of a bolus of [U-2H]pyruvate, lactate production was higher in mice bearing orthotopic LGOG (BT88 and SF10417) and GBM (GS2) tumor xenografts relative to tumor-free mice, suggesting that [U-2H]pyruvate has the potential to monitor TERT expression in vivo. In summary, our study, for the first time, shows the feasibility and utility of [U-2H]pyruvate for in vivo imaging. Importantly, since 2H-MRS can be implemented on clinical scanners, our results provide a novel, non-invasive method of integrating information regarding a fundamental cancer hallmark, i.e. TERT, into glioma patient management.

scholarly journals In vivo imaging techniques: a new era for histochemical analysis

2016 ◽  
Author(s):  
A. Busato ◽  
P. Fumene Feruglio ◽  
P.P. Parnigotto ◽  
P. Marzola ◽  
A. Sbarbati
Keyword(s):  
Magnetic Resonance ◽  
In Vivo Imaging ◽  
Diffusion Tensor ◽  
Technological Development ◽  
Imaging Techniques ◽  
Resonance Spectroscopy ◽  
Preclinical Research ◽  
Tissue Contrast ◽  
Magnetic Resonance Imaging Mri

In vivo imaging techniques can be integrated with classical histochemistry to create an actual histochemistry of water. In particular, Magnetic Resonance Imaging (MRI), an imaging technique primarily used as diagnostic tool in clinical/preclinical research, has excellent anatomical resolution, unlimited penetration depth and intrinsic soft tissue contrast. Thanks to the technological development, MRI is not only capable to provide morphological information but also and more interestingly functional, biophysical and molecular. In this paper we describe the main features of several advanced imaging techniques, such as MRI microscopy, Magnetic Resonance Spectroscopy, functional MRI, Diffusion Tensor Imaging and MRI with contrast agent as a useful support to classical histochemistry.

scholarly journals Cellular Magnetic Resonance Imaging: In Vivo Imaging of Melanoma Cells in Lymph Nodes of Mice

Neoplasia ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 207-216 ◽  
Author(s):  
Paula J Foster ◽  
Elizabeth A Dunn ◽  
Kristina E Karl ◽  
Jonatan A Snir ◽  
Colleen M Nycz ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Lymph Nodes ◽  
In Vivo Imaging ◽  
Melanoma Cells ◽  
Resonance Imaging

Stable Twisted Conformation Aza-BODIPY NIR-II Fluorescent Nanoparticles with Ultra-large Stokes Shift for Imaging-Guided Phototherapy

2022 ◽  
Author(s):  
Youliang Tian ◽  
Huiting Zhou ◽  
Quan Cheng ◽  
Huiping Dang ◽  
Hongyun Qian ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Stokes Shift ◽  
Great Promise ◽  
Deep Penetration ◽  
Fluorescent Nanoparticles ◽  
Spatiotemporal Resolution ◽  
Large Stokes Shift

Fluorescence imaging in the second near-infrared window (NIR-II, 1000–1700 nm) holds great promise for in vivo imaging and imaging-guided phototherapy with deep penetration and high spatiotemporal resolution. It is very...

Co-delivery of chlorin e6 and doxorubicin using PEGylated hollow nanocapsules for ‘all-in-one’ tumor theranostics

Nanomedicine ◽  
2019 ◽  
Vol 14 (17) ◽  
pp. 2273-2292 ◽  
Author(s):  
Qian Li ◽  
Mengmeng Hou ◽  
Junjie Ren ◽  
Shiyu Lu ◽  
Zhigang Xu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Tumor Therapy ◽  
Chlorin E6 ◽  
Precipitation Method ◽  
Antitumor Effects ◽  
Cancer Management ◽  
Therapeutic Outcomes ◽  
Poly Ethylene

Aim: Hollow mesoporous copper sulfide nanocapsules conjugated with poly(ethylene glycol) (PEG), doxorubicin and chlorin e6 (HPDC) were synthesized for fluorescence imaging and multimodal tumor therapy. Materials & methods: HPDC were synthesized by encapsulating chlorin e6 and doxorubicin into PEGylated nanocapsules via a simple precipitation method. The photothermal/photodynamic effects, drug release, cellular uptake, imaging capacities and antitumor effects of the HPDCs were evaluated. Results: This smart nanoplatform is stimulus-responsive toward an acidic microenvironment and near infrared laser irradiation. Moreover, fluorescence imaging-guided and combined photothermal/photodynamic/chemotherapies of tumors were promoted under laser activation and led to efficient tumor ablation, as evidenced by exploring animal models in vivo. Conclusion: HPDCs are expected to serve as potent and reliable nanoagents for achieving superior therapeutic outcomes in cancer management.

Some Aspects Of Nuclear Magnetic Resonance (NMR) In Vivo Imaging

1982 ◽  
Author(s):  
I. R. Young ◽  
A. S. Hall ◽  
A. G. Collins ◽  
J. M. Pennock ◽  
D. H. Spencer ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
In Vivo Imaging ◽  
Nuclear Magnetic

In vivo imaging and tumor therapy with the sodium iodide symporter

2003 ◽  
Vol 90 (6) ◽  
pp. 1079-1086 ◽  
Author(s):  
David Dingli ◽  
Stephen J. Russell ◽  
John C. Morris
Keyword(s):  
In Vivo Imaging ◽  
Sodium Iodide ◽  
Tumor Therapy ◽  
Sodium Iodide Symporter

scholarly journals In Vivo Imaging of Tau Pathology Using Magnetic Resonance Imaging Textural Analysis

2017 ◽  
Vol 11 ◽  
Author(s):  
Niall Colgan ◽  
Balaji Ganeshan ◽  
Ian F. Harrison ◽  
Ozama Ismail ◽  
Holly E. Holmes ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
In Vivo Imaging ◽  
Textural Analysis ◽  
Resonance Imaging ◽  
Tau Pathology

Multifunctional gadolinium-labeled silica-coated Fe3O4 and CuInS2 nanoparticles as a platform for in vivo tri-modality magnetic resonance and fluorescence imaging

2015 ◽  
Vol 3 (14) ◽  
pp. 2873-2882 ◽  
Author(s):  
Jianhua Shen ◽  
Yunfeng Li ◽  
Yihua Zhu ◽  
Xiaoling Yang ◽  
Xiuzhong Yao ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Magnetic Resonance ◽  
Fluorescence Imaging

Gd-labeled, peptide-conjugated Fe3O4 NPs and fluorescent CuInS2 QDs were fabricated for imaging of pancreatic cancer.

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