Block copolymer [(l-GluA-5-BE)-b-(l-AspA-4-BE)]-based nanoflower capsules with thermosensitive morphology and pH-responsive drug release for cancer therapy

2020 ◽  
Vol 8 (40) ◽  
pp. 9258-9268
Author(s):  
Chander Amgoth ◽  
Shuai Chen ◽  
Tirupathi Malavath ◽  
Guping Tang
Keyword(s):  
Amino Acid ◽  
Block Copolymer ◽  
Cancer Therapy ◽  
Drug Release ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Benzyl Ester ◽  
Ph Responsive

Herein, the synthesis of an amino-acid-based di-block copolymer (di-BCP) in-between an l-glutamic acid-5-benzyl ester and L-aspartic acid-4-benzyl ester [(l-GluA-5-BE)-b-(l-AspA-4-BE)] has been reported.

Mitochondrial alkaline pH-responsive drug release mediated by Celastrol loaded glycolipid-like micelles for cancer therapy

Biomaterials ◽  
2018 ◽  
Vol 154 ◽  
pp. 169-181 ◽  
Author(s):  
Yanan Tan ◽  
Yun Zhu ◽  
Yue Zhao ◽  
Lijuan Wen ◽  
Tingting Meng ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Drug Release ◽  
Alkaline Ph ◽  
Ph Responsive

scholarly journals The effect of Cladophora glomerata exudates on the amino acid composition of Cladophora fracta and Rhizoclonium sp.

2019 ◽  
Vol 17 (1) ◽  
pp. 313-324 ◽  
Author(s):  
Marta Pikosz ◽  
Joanna Czerwik-Marcinkowska ◽  
Beata Messyasz
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Amino Acid Composition ◽  
Aspartic Acid ◽  
Defense Mechanisms ◽  
Chemical Stress ◽  
Cladophora Glomerata ◽  
Adaptation To Stress ◽  
Filamentous Green Algae

AbstractFilamentous green algae (FGA) frequently forms dense mats which can be either mono- or polyspecies. While various defense mechanisms of competition in algae are known, little is known about the interactions between different species of FGA. An experiment in controlled laboratory conditions was conducted to gather data on the changes in amino acids (AA) concentrations in FGA species in the presence of exudates from different other species. The aim of the present study was to identify the AA whose concentrations showed significant changes and to assess if the changes could be adaptation to stress conditions. The major constituents of the AA pool in Cladophora glomerata, C. fracta and Rhizoclonium sp. were Glutamic acid (Glu), Aspartic acid (Asp) and Leucine (Leu). In response to chemical stress, that is the increasing presence of exudates, a significant increase in the concentrations Proline (Pro) and Tryptophan (Trp) was noted. The increase in Proline levels was observed in C. fracta and Rhizoclonium in response to chemical stress induced by C. glomerata exudates. As the concentration of exudates increased in the medium, there was a progressive shift in the pattern of AA group in FGA.

MODIFICATION OF GLUTAMIC AND ASPARTIC ACID RESIDUES OF PLASMINOGEN INHIBITS ITS ABILITY TO FORM AN ACTIVE PLASMINOGEN-STREPTOKINASE COMPLEX

1987 ◽  
Author(s):  
D S Holloway ◽  
L Summaria ◽  
R C Wohl ◽  
J A Caprini
Keyword(s):  
Amino Acid ◽  
Reaction Time ◽  
Glutamic Acid ◽  
Binding Site ◽  
Plasminogen Activator ◽  
Aspartic Acid ◽  
Amino Acid Analysis ◽  
Molar Excess ◽  
Maximum Inhibition ◽  
Time Courses

Plasminogen binds to streptokinase in a 1:1 molar complex that has activity as a plasminogen activator. This function of plasminogen, as a cofactor for streptokinase conversion of plasminogen to plasmin, was studied after treatment of Glu-, Lys-, and Mini-plasminogens with 1-ethyl-3-(3-dimethylamino-propyl)-carbodiimide (EDC). Amino acid analysis showed that both aspartic and glutamic acid residues were modified by EDC. Activity of the complex formed between streptokinase and the modified plasminogen was measured using the cfhromogenic substrate H-D-Val-Leu-Lys-pNA. Plasminogen, 2.8 uM, was incubated with 40 mM EDC in 50 mM MES buffer, pH 6.0, at 25°C. At various times while reacting plasminogen with the EDC, aliquots were removed for assay. Plasminogen function was assayed by mixing with a slight molar excess of streptokinase for 1 min at 37 C, followed by reaction with 0.1 M substrate, and absorbancy monitored at 405 nm. Modifications of 20% of the glutamic and aspartic acid residues occurred after treatment of plasminogen with EDC. This resulted in 80 to 90% inhibition of activation in all three types of plasminogen. Glu- and Lys-plasminogens reacted more quickly with the EDC than did Mini-plasminogen, with 50% inhibition occurring after 16 ± 5, 16 ± 4, and 67 ± 13 min reaction time with EDC for Glu-, Lys-, and Mini-plasminogens, respectively. Maximum inhibition of activation occurred within 1 hr reaction with EDC for Glu- and Lys-plasminogens but required 2.5 hr for Mini-plasminogen. The time courses for activation inhibition and the modification of the glutamic and aspartic acids of treated Mini-plasminogen were compared. A significant decrease in activation occurred (52%) concomitant with modification of only one or two glutamic acids, followed on further reaction with EDC by more loss of activatability as more glutamic and aspartic acids were modified. The inability of plasminogen to form an active plasminogen-streptokinase complex after modification with EDC indicates that glutamic and aspartic acid residues are involved in the binding site of plasminogen for streptokinase.

A pH-responsive prodrug for real-time drug release monitoring and targeted cancer therapy

2014 ◽  
Vol 50 (80) ◽  
pp. 11852-11855 ◽  
Author(s):  
Shi-Ying Li ◽  
Li-Han Liu ◽  
Hui-Zhen Jia ◽  
Wen-Xiu Qiu ◽  
Lei Rong ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Drug Release ◽  
Real Time ◽  
Targeted Cancer Therapy ◽  
Ph Responsive

pH-Responsive Assembly of Gold Nanoparticles and “Spatiotemporally Concerted” Drug Release for Synergistic Cancer Therapy

ACS Nano ◽  
2013 ◽  
Vol 7 (4) ◽  
pp. 3388-3402 ◽  
Author(s):  
Jutaek Nam ◽  
Wan-Geun La ◽  
Sekyu Hwang ◽  
Yeong Su Ha ◽  
Nokyoung Park ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cancer Therapy ◽  
Drug Release ◽  
Ph Responsive

A pH-responsive AIE nanoprobe as a drug delivery system for bioimaging and cancer therapy

2015 ◽  
Vol 3 (37) ◽  
pp. 7401-7407 ◽  
Author(s):  
Haibo Wang ◽  
Gongyan Liu ◽  
Shihua Dong ◽  
Junjie Xiong ◽  
Zongliang Du ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cancer Therapy ◽  
Drug Release ◽  
Drug Delivery System ◽  
Delivery System ◽  
Cellular Imaging ◽  
Ph Responsive ◽  
Triggered Drug Release

A multifunctional drug delivery system with AIE character was designed and constructed for simultaneous cellular imaging and pH-triggered drug release.

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