Osteopontin sequence modified mesoporous calcium silicate scaffolds to promote angiogenesis in bone tissue regeneration

2020 ◽  
Vol 8 (27) ◽  
pp. 5849-5861
Author(s):  
Min Zhu ◽  
He He ◽  
Qingxi Meng ◽  
Yufang Zhu ◽  
Xiaojian Ye ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Calcium Silicate ◽  
Bone Tissue Regeneration ◽  
Surface Grafting

Surface grafting and encapsulation of SVVYGLR peptides in MCS promote vessel/bone formation all over the scaffold.

Fabrication, characterization, bioactivity, and biocompatibility of novel mesoporous calcium silicate/polyetheretherketone composites

RSC Advances ◽  
2016 ◽  
Vol 6 (62) ◽  
pp. 57131-57137 ◽  
Author(s):  
G. F. Hu ◽  
R. F. Quan ◽  
Y. M. Chen ◽  
D. W. Bi ◽  
X. S. Jiang ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Calcium Silicate ◽  
Bone Tissue Regeneration

Composite consisting of polyetheretherketone and mesoporous calcium silicate were fabricated. The composite with improved hydrophilicity, bioactivity and biocompatibility might be a great candidate for bone tissue regeneration.

scholarly journals Polydopamine-modified poly(l-lactic acid) nanofiber scaffolds immobilized with an osteogenic growth peptide for bone tissue regeneration

RSC Advances ◽  
2019 ◽  
Vol 9 (21) ◽  
pp. 11722-11736 ◽  
Author(s):  
Yong Liu ◽  
Changlu Xu ◽  
Yong Gu ◽  
Xiaofeng Shen ◽  
Yanxia Zhang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bone Formation ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration ◽  
Nanofiber Scaffolds

Polydopamine-modified PLLA nanofiber scaffolds immobilized with osteogenic growth peptide were designed and prepared for promoting bone formation.

scholarly journals Icaritin Enhancing Bone Formation Initiated by Sub-Microstructured Calcium Phosphate Ceramic for Critical Size Defect Repair

2020 ◽  
Vol 7 ◽  
Author(s):  
Haitao Peng ◽  
Jianxiao Li ◽  
Yanan Xu ◽  
Guoyu Lv
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Calcium Phosphate Ceramic ◽  
Dose Dependent

Adequate bone tissue regeneration has been challenging to achieve at critical-sized bone defects caused by disease. Bone tissue engineering using a combination of scaffolds and bioactive factors provides new hope for the treatment of this extreme condition. Icaritin, a herb-derived chemical, has shown its ability to enhance bone formation both in vitro and in vivo, and it has been found that sub-micron surface structure instructs bone formation in calcium phosphate ceramics (CaPs). Here, we evaluated the possibility of using a submicron surface structured CaP ceramic as the carrier of icaritin for bone tissue regeneration in critical-sized bone defects. Icaritin, an herb-derived chemical, was loaded into a submicron surface structured porous calcium phosphate ceramic (Ø12.8 × 3 mm) to get samples with 0, 10, 50, 250, and 1,250 µg icaritin per CaP disc (M0, M10, M50, M250, M1250 groups, respectively). In vitro evaluation with the certain dosages correlated to those released from the samples showed a dose-dependent enhancement of osteogenic differentiation and mineralization of human bone marrow stromal cells with the presence of osteogenic factors in the culture medium, indicating icaritin is an osteopromotive factor. After intramuscular implantation of the samples in dogs for 8 weeks, a dose-dependent of bone formation was seen with enhanced bone formation at the dosage of 50 and 250 µg. To evaluate the in vivo osteogenic potentials of icaritin-containing CaP ceramic scaffolds in the orthopedic site, a 12.8 mm calvarial defect model in rabbits was established. Micro-computed tomography (micro-CT) and histology results at weeks 4, 8 and 12 post-surgery showed more newly formed bone in M250 group, with correspondingly more new vessel ingrowth. The results presented herein suggested that being osteopromotive, icaritin could enhance bone formation initiated by sub-microstructured CaP ceramics and the CaP ceramics scaffold incorporating icaritin is a promising biomaterial for the treatment of critical-sized defect.

Microenvironment construction of strontium–calcium-based biomaterials for bone tissue regeneration: the equilibrium effect of calcium to strontium

2018 ◽  
Vol 6 (15) ◽  
pp. 2332-2339 ◽  
Author(s):  
Huixu Xie ◽  
Zhipeng Gu ◽  
Yan He ◽  
Jia Xu ◽  
Chun Xu ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration ◽  
Beneficial Effects ◽  
Equilibrium Effect

Strontium-doped calcium phosphate-based biomaterials have gained increased recognition due to their beneficial effects on bone formation.

scholarly journals Effect of Honeycomb β-TCP Geometrical Structure on Bone Tissue Regeneration in Skull Defect

Materials ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 4761 ◽  
Author(s):  
Toshiyuki Watanabe ◽  
Kiyofumi Takabatake ◽  
Hidetsugu Tsujigiwa ◽  
Satoko Watanabe ◽  
Ryoko Nakagiri ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Histological Finding ◽  
Collagen Gel ◽  
Bone Tissue Regeneration ◽  
Bone Microenvironment ◽  
Skull Defects ◽  
Collagen Group ◽  
Skull Bone Defect

The effect of the geometric structure of artificial biomaterials on skull regeneration remains unclear. In a previous study, we succeeded in developing honeycomb β-tricalcium phosphate (β-TCP), which has through-and-through holes and is able to provide the optimum bone microenvironment for bone tissue regeneration. We demonstrated that β-TCP with 300-μm hole diameters induced vigorous bone formation. In the present study, we investigated how differences in hole directions of honeycomb β-TCP (horizontal or vertical holes) influence bone tissue regeneration in skull defects. Honeycomb β-TCP with vertical and horizontal holes was loaded with BMP-2 using Matrigel and Collagen gel as carriers, and transplanted into skull bone defect model rats. The results showed that in each four groups (Collagen alone group, Matrigel alone group, Collagen + BMP group and Matrigel + BMP-2), vigorous bone formation was observed on the vertical β-TCP compared with horizontal β-TCP. The osteogenic area was larger in the Matrigel groups (with and without BMP-2) than in the Collagen group (with and without BMP-2) in both vertical β-TCP and horizontal β-TCP. However, when BMP-2 was added, the bone formation area was not significantly different between the Collagen group and the Matrigel group in the vertical β-TCP. Histological finding showed that, in vertical honeycomb β-TCP, new bone formation extended to the upper part of the holes and was observed from the dura side to the periosteum side as added to the inner walls of the holes. Therefore, we can control efficient bone formation by creating a bone microenvironment provided by vertical honeycomb β-TCP. Vertical honeycomb β-TCP has the potential to be an excellent biomaterial for bone tissue regeneration in skull defects and is expected to have clinical applications.

scholarly journals Scaffold Structural Microenvironmental Cues to Guide Tissue Regeneration in Bone Tissue Applications

Nanomaterials ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 960 ◽  
Author(s):  
Xuening Chen ◽  
Hongyuan Fan ◽  
Xiaowei Deng ◽  
Lina Wu ◽  
Tao Yi ◽  
...  
Keyword(s):  
Bone Formation ◽  
Pore Structure ◽  
Surface Topography ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration ◽  
New Bone Formation ◽  
Cell Fates ◽  
Physico Chemical ◽  
Tissue Cells

In the process of bone regeneration, new bone formation is largely affected by physico-chemical cues in the surrounding microenvironment. Tissue cells reside in a complex scaffold physiological microenvironment. The scaffold should provide certain circumstance full of structural cues to enhance multipotent mesenchymal stem cell (MSC) differentiation, osteoblast growth, extracellular matrix (ECM) deposition, and subsequent new bone formation. This article reviewed advances in fabrication technology that enable the creation of biomaterials with well-defined pore structure and surface topography, which can be sensed by host tissue cells (esp., stem cells) and subsequently determine cell fates during differentiation. Three important cues, including scaffold pore structure (i.e., porosity and pore size), grain size, and surface topography were studied. These findings improve our understanding of how the mechanism scaffold microenvironmental cues guide bone tissue regeneration.

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