Multi-signal amplification electrochemical DNA biosensor based on exonuclease III and tetraferrocene

2020 ◽  
Vol 8 (18) ◽  
pp. 4143-4150
Author(s):  
Zhaojiang Yin ◽  
Hanfeng Cui ◽  
Qingxia Shu ◽  
Chen Jin ◽  
Yan Lin ◽  
...  
Keyword(s):  
Benzoic Acid ◽  
Electrochemical Sensor ◽  
Signal Amplification ◽  
Dna Biosensor ◽  
Exonuclease Iii ◽  
Electrochemical Dna Biosensor ◽  
Exo Iii

A new and efficient signal marker, 3,5-bis(3,5-bisferrocenethoxybenzyloxy)benzoic acid, was firstly synthesized and used for labeling. Construct a homogeneous electrochemical sensor with Exo III assisting the target cycle.

scholarly journals Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy

Nanomaterials ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 104
Author(s):  
Fuyuan Zhang ◽  
Linyang Liu ◽  
Shengnan Ni ◽  
Jiankang Deng ◽  
Guo-Jun Liu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Aflatoxin M1 ◽  
Exonuclease Iii ◽  
Sensor Performance ◽  
Milk Samples ◽  
Increased Sensitivity ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Optimized Conditions ◽  
Magnetic Nanobeads

In order to satisfy the need for sensitive detection of Aflatoxin M1 (AFM1), we constructed a simple and signal-on fluorescence aptasensor based on an autocatalytic Exonuclease III (Exo III)-assisted signal amplification strategy. In this sensor, the DNA hybridization on magnetic nanobeads could be triggered by the target AFM1, resulting in the release of a single-stranded DNA to induce an Exo III-assisted signal amplification, in which numerous G-quadruplex structures would be produced and then associated with the fluorescent dye to generate significantly amplified fluorescence signals resulting in the increased sensitivity. Under the optimized conditions, this aptasensor was able to detect AFM1 with a practical detection limit of 9.73 ng kg−1 in milk samples. Furthermore, the prepared sensor was successfully used for detection of AFM1 in the commercially available milk samples with the recovery percentages ranging from 80.13% to 108.67%. Also, the sensor performance was evaluated by the commercial immunoassay kit with satisfactory results.

A novel electrochemical DNA biosensor for transgenic soybean detection based on triple signal amplification

2019 ◽  
Vol 1078 ◽  
pp. 24-31 ◽  
Author(s):  
Dongli Chen ◽  
Meng Zhang ◽  
Mingyi Ma ◽  
Hong Hai ◽  
Jianping Li ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dna Biosensor ◽  
Transgenic Soybean ◽  
Electrochemical Dna Biosensor

A facile signal-on electrochemical DNA sensing platform for ultrasensitive detection of pathogenic bacteria based on Exo III-assisted autonomous multiple-cycle amplification

The Analyst ◽  
2019 ◽  
Vol 144 (9) ◽  
pp. 3023-3029 ◽  
Author(s):  
Qianqian Pei ◽  
Xiaolei Song ◽  
Su Liu ◽  
Jingfeng Wang ◽  
Xueqi Leng ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Dna Biosensor ◽  
Ultrasensitive Detection ◽  
Dna Sensing ◽  
Electrochemical Dna Biosensor ◽  
Sensing Platform ◽  
Multiple Cycle ◽  
Exo Iii

A facile signal-on electrochemical DNA biosensor for ultrasensitive detection of pathogenic bacteria based on Exo III-assisted autonomous multiple-cycle amplification.

A New Photoelectrochemical Biosensor based on FeOOH and Exonuclease III-aided Dual Recycling Signal Amplification for HPV-16 Detection

2021 ◽  
Author(s):  
Yanlin Wang ◽  
Lingying Xia ◽  
Xuelian Xiang ◽  
Ruo Yuan ◽  
Shangping Wei
Keyword(s):  
Human Papillomavirus ◽  
Signal Amplification ◽  
Iron Oxyhydroxide ◽  
Hpv 16 ◽  
Exonuclease Iii ◽  
Human Papillomavirus 16 ◽  
Photoelectrochemical Biosensor ◽  
Exo Iii

Herein, based on iron oxyhydroxide (FeOOH) as the photoactive material and exonuclease III (Exo III)-aided dual recycling signal amplification, a new photoelectrochemical (PEC) biosensor was successfully developed for human papillomavirus-16...

Label-free detection of nicotinamide adenine dinucleotide based on ligation-triggered exonuclease III-assisted signal amplification

RSC Advances ◽  
2015 ◽  
Vol 5 (105) ◽  
pp. 86625-86630 ◽  
Author(s):  
Haiyan Zhao ◽  
Lei Wang ◽  
Xingti Liu ◽  
Zhiyue Gao ◽  
Wei Jiang
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Signal Amplification ◽  
Nicotinamide Adenine ◽  
Label Free ◽  
Exonuclease Iii ◽  
Label Free Detection ◽  
Exo Iii ◽  
Amplification Strategy

Schematic illustration of the Exo III-assisted amplification strategy for NAD+ detection.

Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4267-4272 ◽  
Author(s):  
Biqing Bao ◽  
Yanrui Pan ◽  
Bingbing Gu ◽  
Jia Chen ◽  
Yu Xu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Conjugated Polyelectrolytes ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Exo Iii ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Cascade Amplification

A ratiometric and cascade amplification strategy that combines the signal amplification and effecitive FRET property of CPEs with the Exo III-assisted target recycling method has been developed for DNA detection.

Ultrasensitive and simple fluorescence biosensor for detection of the mecA gene of Staphylococcus aureus by using an exonuclease III-assisted cascade signal amplification strategy

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5670-5675 ◽  
Author(s):  
Qiong Li ◽  
Danhua Zhou ◽  
Jiafeng Pan ◽  
Zhi Liu ◽  
Junhua Chen
Keyword(s):  
Staphylococcus Aureus ◽  
Signal Amplification ◽  
Meca Gene ◽  
Exonuclease Iii ◽  
G Quadruplex ◽  
Exo Iii ◽  
Fluorescence Biosensor ◽  
Amplification Strategy

An ultrasensitive biosensor was developed for the detection of the mecA gene of Staphylococcus aureus based on the Exo III and G-quadruplex.

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