scholarly journals Reorientable fluorinated aryl rings in triangular channel Fe-MOFs: an investigation on CO2–matrix interactions

2020 ◽  
Vol 8 (22) ◽  
pp. 11406-11413
Author(s):  
J. Perego ◽  
C. X. Bezuidenhout ◽  
A. Pedrini ◽  
S. Bracco ◽  
M. Negroni ◽  
...  
Keyword(s):  
Triangular Channel ◽  
Matrix Interactions

Flexible aryl rings in fluorinated Fe-MOFs point C–F dipoles towards the corner of triangular channels capturing efficiently CO2 molecules.

Use of F9 teratocarcinoma STEM cells to study cell-matrix interactions in the early mouse embryo

1992 ◽  
Vol 50 (1) ◽  
pp. 602-603
Author(s):  
Marc Lenburg ◽  
Rulang Jiang ◽  
Lengya Cheng ◽  
Laura Grabel
Keyword(s):  
Mouse Embryo ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Cell Types ◽  
Embryoid Bodies ◽  
F9 Cells ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions ◽  
F9 Teratocarcinoma

We are interested in defining the cell-cell and cell-matrix interactions that help direct the differentiation of extraembryonic endoderm in the peri-implantation mouse embryo. At the blastocyst stage the mouse embryo consists of an outer layer of trophectoderm surrounding the fluid-filled blastocoel cavity and an eccentrically located inner cell mass. On the free surface of the inner cell mass, facing the blastocoel cavity, a layer of primitive endoderm forms. Primitive endoderm then generates two distinct cell types; parietal endoderm (PE) which migrates along the inner surface of the trophectoderm and secretes large amounts of basement membrane components as well as tissue-type plasminogen activator (tPA), and visceral endoderm (VE), a columnar epithelial layer characterized by tight junctions, microvilli, and the synthesis and secretion of α-fetoprotein. As these events occur after implantation, we have turned to the F9 teratocarcinoma system as an in vitro model for examining the differentiation of these cell types. When F9 cells are treated in monolayer with retinoic acid plus cyclic-AMP, they differentiate into PE. In contrast, when F9 cells are treated in suspension with retinoic acid, they form embryoid bodies (EBs) which consist of an outer layer of VE and an inner core of undifferentiated stem cells. In addition, we have established that when VE containing embryoid bodies are plated on a fibronectin coated substrate, PE migrates onto the matrix and this interaction is inhibited by RGDS as well as antibodies directed against the β1 integrin subunit. This transition is accompanied by a significant increase in the level of tPA in the PE cells. Thus, the outgrowth system provides a spatially appropriate model for studying the differentiation and migration of PE from a VE precursor.

Cell matrix interactions in asthma

1997 ◽  
Vol 27 (1) ◽  
pp. 22-27
Author(s):  
K. GOLDRING ◽  
J. A. WARNER
Keyword(s):  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions

SHCA PHOSPHOTYROSINE DERIVED SIGNALING IS REQUIRED FOR THE MAINTENANCE OF CARDIAC FUNCTION

2008 ◽  
Vol 31 (4) ◽  
pp. 23
Author(s):  
Rachel Vanderlaan ◽  
Rod Hardy ◽  
Golam Kabir ◽  
Peter Back ◽  
A J Pawson
Keyword(s):  
Extracellular Matrix ◽  
Cardiac Function ◽  
Tyrosine Kinases ◽  
Sh2 Domain ◽  
Scaffolding Protein ◽  
Restricted Domain ◽  
Downstream Signaling ◽  
Matrix Interactions ◽  
Extracellular Matrix Interactions ◽  
Ptb Domain

Background: ShcA, a scaffolding protein, generates signalspecificity by docking to activated tyrosine kinases through distinct phosphotyrosine recognition motifs, while mediating signal complexity through formation of diverse downstream phosphotyrosine complexes. Mammalian ShcA encodes 3 isoforms having a modular architecture of a PTB domain and SH2 domain, separated by a CH1 region containing tyrosine phosphorylation sites important in Ras-MAPK activation. Objective and Methods: ShcA has a necessary role in cardiovascular development^1,2. However, the role of ShcA in the adult myocardium is largely unknown, also unclear, is how ShcA uses its signaling modules to mediate downstream signaling. To this end, cre/loxP technology was employed to generate a conditional ShcA allele series. The myocardial specific ShcA KO (ShcA CKO) and myocardial restricted domain mutant KI mice were generated using cre expressed from the mlc2v locus^3 coupled with the ShcA floxed allele and in combination with the individual ShcA domain mutant KI alleles^2. Results: ShcACKO mice develop a dilated cardiomyopathy phenotype by 3 months of life, typified by depressed cardiac function and enlarged chamber dimensions. Isolated cardiomyocytes from ShcA CKO mice have preserved contractility indicating an uncoupling between global heart function and single myocyte contractile mechanics. Force-length experiments suggest that the loss of shcAmediates the uncoupling through deregulation of extracellular matrix interactions. Subsequent, analysis of the ShcA myocardial restricted domain mutant KImice suggests that ShcA requires PTB domain docking to upstream tyrosine kinases and subsequent phosphorylation of the CH1 tyrosines important for downstream signaling. Conclusion: ShcA is required for proper maintenance of cardiac function, possibly regulation of extracellular matrix interactions. References: 1. Lai KV, Pawson AJ. The ShcA phosphotyrosine docking protein sensitizescardiovascular signaling in the mouse embryo. Genes and Dev 2000;14:1132-45. 2. Hardy WR. et al. Combinatorial ShcA docking interactions supportdiversity in tissue morphogenesis. Science2007;317:251-6. 3.Minamisawa, s. et al. A post-transcriptional compensatory pathway inheterozygous ventricular myosin light chain 2-deficient mice results in lack ofgene dosage effect during normal cardiac growth or hypertrophy. J Biol Chem 1999;274:10066-70.

Moisture-Matrix Interactions in Polymer Based Composite Materials

1995 ◽  
Vol 50 (4) ◽  
pp. 551-571 ◽  
Author(s):  
G. Mensitieri ◽  
M. A. Del Nobile ◽  
A. Apicella ◽  
L. Nicolais
Keyword(s):  
Composite Materials ◽  
Matrix Interactions

scholarly journals A computational framework for modeling cell–matrix interactions in soft biological tissues

2021 ◽  
Author(s):  
Jonas F. Eichinger ◽  
Maximilian J. Grill ◽  
Iman Davoodi Kermani ◽  
Roland C. Aydin ◽  
Wolfgang A. Wall ◽  
...  
Keyword(s):  
Soft Tissues ◽  
Three Dimensional ◽  
Biological Tissues ◽  
Computational Framework ◽  
Cell Matrix ◽  
Physiological Processes ◽  
Matrix Interactions ◽  
Mechanical Homeostasis ◽  
Cell Matrix Interactions ◽  
Short Time

AbstractLiving soft tissues appear to promote the development and maintenance of a preferred mechanical state within a defined tolerance around a so-called set point. This phenomenon is often referred to as mechanical homeostasis. In contradiction to the prominent role of mechanical homeostasis in various (patho)physiological processes, its underlying micromechanical mechanisms acting on the level of individual cells and fibers remain poorly understood, especially how these mechanisms on the microscale lead to what we macroscopically call mechanical homeostasis. Here, we present a novel computational framework based on the finite element method that is constructed bottom up, that is, it models key mechanobiological mechanisms such as actin cytoskeleton contraction and molecular clutch behavior of individual cells interacting with a reconstructed three-dimensional extracellular fiber matrix. The framework reproduces many experimental observations regarding mechanical homeostasis on short time scales (hours), in which the deposition and degradation of extracellular matrix can largely be neglected. This model can serve as a systematic tool for future in silico studies of the origin of the numerous still unexplained experimental observations about mechanical homeostasis.

scholarly journals Numerical and Theoretical Study of Performance and Mechanical Behavior of PEM-FC Using Innovative Channel Geometrical Configurations

2021 ◽  
Vol 11 (12) ◽  
pp. 5597
Author(s):  
Hussein A. Z. AL-bonsrulah ◽  
Mohammed J. Alshukri ◽  
Ammar I. Alsabery ◽  
Ishak Hashim
Keyword(s):  
Fuel Cell ◽  
Cross Section ◽  
Rectangular Channel ◽  
Compressive Load ◽  
Proton Exchange ◽  
Channel Cross Section ◽  
Channel Height ◽  
Cross Sectional ◽  
Triangular Channel ◽  
Higher Power

Proton exchange membrane fuel cell (PEM-FC) aggregation pressure causes extensive strains in cell segments. The compression of each segment takes place through the cell modeling method. In addition, a very heterogeneous compressive load is produced because of the recurrent channel rib design of the dipole plates, so that while high strains are provided below the rib, the domain continues in its initial uncompressed case under the ducts approximate to it. This leads to significant spatial variations in thermal and electrical connections and contact resistances (both in rib–GDL and membrane–GDL interfaces). Variations in heat, charge, and mass transfer rates within the GDL can affect the performance of the fuel cell (FC) and its lifetime. In this paper, two scenarios are considered to verify the performance and lifetime of the PEM-FC using different innovative channel geometries. The first scenario is conducted by adopting a constant channel height (H = 1 mm) for all the differently shaped channels studied. In contrast, the second scenario is conducted by taking a constant channel cross-sectional area (A = 1 mm2) for all the studied channels. Therefore, a computational fluid dynamics model (CFD) for a PEM fuel cell is formed through the assembly of FC to simulate the pressure variations inside it. The simulation results showed that a triangular cross-section channel provided the uniformity of the pressure distribution, with lower deformations and lower mechanical stresses. The analysis helped gain insights into the physical mechanisms that lead to the FC’s durability and identify important parameters under different conditions. The model shows that it can assume the intracellular pressure configuration toward durability and appearance containing limited experimental data. The results also proved that the better cell voltage occurs in the case of the rectangular channel cross-section, and therefore, higher power from the FC, although its durability is much lower compared to the durability of the triangular channel. The results also showed that the rectangular channel cross-section gave higher cell voltages, and therefore, higher power (0.63 W) from the fuel cell, although its durability is much lower compared to the durability of the triangular channel. Therefore, the triangular channel gives better performance compared to other innovative channels.

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