Recent advances in AFM-based biological characterization and applications at multiple levels

Soft Matter ◽  
2020 ◽  
Vol 16 (39) ◽  
pp. 8962-8984
Author(s):  
Wenfeng Liang ◽  
Haohao Shi ◽  
Xieliu Yang ◽  
Junhai Wang ◽  
Wenguang Yang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Natural Environments ◽  
Biological Characterization ◽  
Force Microscopy ◽  
The Past ◽  
Atomic Force ◽  
Wide Range ◽  
Multiple Levels

Atomic force microscopy (AFM) has found a wide range of bio-applications in the past few decades due to its ability to measure biological samples in natural environments at a high spatial resolution.

High-Spatial-Resolution Topographic Imaging and Dimer Distance Analysis of Si(100)-(2×1) Using Noncontact Atomic Force Microscopy

2008 ◽  
Vol 47 (7) ◽  
pp. 6085-6087 ◽  
Author(s):  
Daisuke Sawada ◽  
Takashi Namikawa ◽  
Masuhiro Hiragaki ◽  
Yoshiaki Sugimoto ◽  
Masayuki Abe ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Force Microscopy ◽  
Distance Analysis ◽  
Atomic Force ◽  
Topographic Imaging

scholarly journals Acquisition of time–frequency localized mechanical properties of biofilms and single cells with high spatial resolution

Nanoscale ◽  
2019 ◽  
Vol 11 (18) ◽  
pp. 8918-8929 ◽  
Author(s):  
Enrique A. López-Guerra ◽  
Hongchen Shen ◽  
Santiago D. Solares ◽  
Danmeng Shuai
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Single Cells ◽  
Force Microscopy ◽  
Time Frequency ◽  
Viscoelastic Analysis ◽  
Atomic Force ◽  
Frequency Window

History-dependent viscoelastic analysis by atomic force microscopy delivers highly spatial-localized biofilm properties within a wide time–frequency window.

Novel Method for High-Spatial-Resolution Chemical Analysis of Buried Polymer-Metal Interface: Atomic Force Microscopy-Infrared (AFM-IR) Spectroscopy with Low-Angle Microtomy

2021 ◽  
pp. 000370282110071
Author(s):  
Naoki Baden
Keyword(s):  
Atomic Force Microscopy ◽  
Ir Spectroscopy ◽  
Chemical Analysis ◽  
Cross Section ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Force Microscopy ◽  
Atomic Force ◽  
Polymer Metal ◽  
Metal Interfaces

There is a great need for the analysis of the chemical composition, structure, functional groups, and interactions at polymer-metal interfaces in terms of adhesion, corrosion, and insulation. Although atomic force microscopy-based infrared (AFM-IR) spectroscopy can provide chemical analysis with nanoscale spatial resolution, it generally requires to thin a sample to be placed on a substrate that has low absorption of infrared light and high thermal conductivity, which is often difficult for samples that contain hard materials such as metals. This study demonstrates that the combination of AFM-IR with low-angle microtomy (LAM) sample preparation can analyze buried polymer-metal interfaces with higher spatial resolution than that with the conventional sample preparation of a thick vertical cross-section. In the LAM of a polymer layer on a metal substrate, the polymer layer is tapered to be thin in the vicinity of the interface, and thus, sample thinning is not required. An interface between an epoxyacrylate layer and copper wire in a flexible printed circuit cable was measured using this method. A carboxylate interphase layer with a thickness of ∼130 nm was clearly visualized at the interface, and its spectrum was obtained without any signal contamination from the neighboring epoxyacrylate, which was difficult to achieve on a thick vertical cross-section. The combination of AFM-IR with LAM is a simple and useful method for high-spatial-resolution chemical analysis of buried polymer-metal interfaces.

Force dependences for the definition of the atomic force microscopy spatial resolution

1988 ◽  
Vol 132 (6-7) ◽  
pp. 354-358 ◽  
Author(s):  
Yu.N. Moiseev ◽  
V.M. Mostepanenko ◽  
V.I. Panov ◽  
I.Yu. Sokolov
Keyword(s):  
Atomic Force Microscopy ◽  
Spatial Resolution ◽  
Force Microscopy ◽  
Atomic Force ◽  
Definition Of

scholarly journals Wide range local resistance imaging on fragile materials by conducting probe atomic force microscopy in intermittent contact mode

2016 ◽  
Vol 108 (24) ◽  
pp. 243101 ◽  
Author(s):  
Aymeric Vecchiola ◽  
Pascal Chrétien ◽  
Sophie Delprat ◽  
Karim Bouzehouane ◽  
Olivier Schneegans ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Local Resistance ◽  
Contact Mode ◽  
Force Microscopy ◽  
Atomic Force ◽  
Wide Range ◽  
Intermittent Contact

scholarly journals Optical force microscopy: combining light with atomic force microscopy for nanomaterial identification

Nanophotonics ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 1659-1671
Author(s):  
Nusrat Jahan ◽  
Hanwei Wang ◽  
Shensheng Zhao ◽  
Arkajit Dutta ◽  
Hsuan-Kai Huang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Morphology ◽  
Spatial Resolution ◽  
Optical Force ◽  
Scanning Probe ◽  
Force Microscopy ◽  
Atomic Force ◽  
Recent Advances ◽  
Spectroscopic Information

AbstractScanning probe techniques have evolved significantly in recent years to detect surface morphology of materials down to subnanometer resolution, but without revealing spectroscopic information. In this review, we discuss recent advances in scanning probe techniques that capitalize on light-induced forces for studying nanomaterials down to molecular specificities with nanometer spatial resolution.

scholarly journals BioAFMviewer: An interactive interface for simulated AFM scanning of biomolecular structures and dynamics

2020 ◽  
Vol 16 (11) ◽  
pp. e1008444
Author(s):  
Romain Amyot ◽  
Holger Flechsig
Keyword(s):  
Molecular Structure ◽  
Atomic Force Microscopy ◽  
Spatial Resolution ◽  
High Speed ◽  
Graphical Representation ◽  
Force Microscopy ◽  
Interactive Interface ◽  
Atomic Force ◽  
And Control

We provide a stand-alone software, the BioAFMviewer, which transforms biomolecular structures into the graphical representation corresponding to the outcome of atomic force microscopy (AFM) experiments. The AFM graphics is obtained by performing simulated scanning over the molecular structure encoded in the corresponding PDB file. A versatile molecular viewer integrates the visualization of PDB structures and control over their orientation, while synchronized simulated scanning with variable spatial resolution and tip-shape geometry produces the corresponding AFM graphics. We demonstrate the applicability of the BioAFMviewer by comparing simulated AFM graphics to high-speed AFM observations of proteins. The software can furthermore process molecular movies of conformational motions, e.g. those obtained from servers which model functional transitions within a protein, and produce the corresponding simulated AFM movie. The BioAFMviewer software provides the platform to employ the plethora of structural and dynamical data of proteins in order to help in the interpretation of biomolecular AFM experiments.

scholarly journals Atomic Force Microscopy on Biological Materials Related to Pathological Conditions

Scanning ◽  
2019 ◽  
Vol 2019 ◽  
pp. 1-25 ◽  
Author(s):  
Andreas Stylianou ◽  
Stylianos-Vasileios Kontomaris ◽  
Colin Grant ◽  
Eleni Alexandratou
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
Surface Characterization ◽  
Amyloid Fibrils ◽  
Biological Materials ◽  
Force Microscopy ◽  
Fibrous Protein ◽  
Atomic Force ◽  
Pathological Conditions ◽  
Wide Range

Atomic force microscopy (AFM) is an easy-to-use, powerful, high-resolution microscope that allows the user to image any surface and under any aqueous condition. AFM has been used in the investigation of the structural and mechanical properties of a wide range of biological matters including biomolecules, biomaterials, cells, and tissues. It provides the capacity to acquire high-resolution images of biosamples at the nanoscale and allows at readily carrying out mechanical characterization. The capacity of AFM to image and interact with surfaces, under physiologically relevant conditions, is of great importance for realistic and accurate medical and pharmaceutical applications. The aim of this paper is to review recent trends of the use of AFM on biological materials related to health and sickness. First, we present AFM components and its different imaging modes and we continue with combined imaging and coupled AFM systems. Then, we discuss the use of AFM to nanocharacterize collagen, the major fibrous protein of the human body, which has been correlated with many pathological conditions. In the next section, AFM nanolevel surface characterization as a tool to detect possible pathological conditions such as osteoarthritis and cancer is presented. Finally, we demonstrate the use of AFM for studying other pathological conditions, such as Alzheimer’s disease and human immunodeficiency virus (HIV), through the investigation of amyloid fibrils and viruses, respectively. Consequently, AFM stands out as the ideal research instrument for exploring the detection of pathological conditions even at very early stages, making it very attractive in the area of bio- and nanomedicine.

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