Membrane determinants for the passive translocation of analytes through droplet interface bilayers

Soft Matter ◽  
2020 ◽  
Vol 16 (25) ◽  
pp. 5970-5980
Author(s):  
Vincent Faugeras ◽  
Olivier Duclos ◽  
Didier Bazile ◽  
Abdou Rachid Thiam
Keyword(s):  
Small Molecules ◽  
Cell Membranes ◽  
Droplet Interface Bilayer

Identifying droplet interface bilayer conditions reproducing the permeability of cell membranes to small molecules.

TRANSPORT OF SMALL MOLECULES ACROSS CELL MEMBRANES: WATER CHANNELS AND UREA TRANSPORTERS

2002 ◽  
Vol 26 (3) ◽  
pp. 146-157 ◽  
Author(s):  
Barbara E. Goodman
Keyword(s):  
Mathematical Models ◽  
Small Molecules ◽  
Membrane Transport ◽  
Lipid Bilayer ◽  
Cell Membranes ◽  
Water Channels ◽  
Transport Proteins ◽  
Transmembrane Transport

How do small hydrophilic nonelectrolytes cross cell membranes? Which pathways are most important for small lipid insoluble molecules to cross cell membranes? These are questions that have been basic to membrane transport physiology for decades. More importantly, these are questions whose answers have changed significantly within the last 10 years. This review discusses the evidence that pathways other than the lipid bilayer itself exist for the transport across cell membranes of specific small hydrophilic nonelectrolytes. The description begins with briefly analyzing the relevance of well accepted basic mathematical models for transport for understanding the permeability of representative physiologically important molecules across actual cell membranes. Particular emphasis is placed on describing recently discovered proteins that facilitate the transport of some of the smallest physiologically important lipid-insoluble molecules, water, and urea. Evidence also exists for transport proteins that selectively enhance the transmembrane transport of other small lipid-insoluble molecules. Do nonselective pores for small molecules exist in cell membranes?

scholarly journals Interaction of polypeptide hormones with lipid momolayers

1968 ◽  
Vol 108 (3) ◽  
pp. 369-373 ◽  
Author(s):  
R. S. Snart ◽  
N. N. Sanyal
Keyword(s):  
Angiotensin Ii ◽  
Small Molecules ◽  
Cell Membranes ◽  
Arginine Vasotocin ◽  
Lipid Monolayer ◽  
Lipid Monolayers ◽  
Suitable Model ◽  
Adsorbed Films ◽  
Polypeptide Hormones

1. The interaction between lipid monolayers spread on the surface of water and oxytocin, [8-arginine]-vasotocin and [1-asparagine-5-valine]-angiotensin II in the subphase was investigated in a Langmuir surface trough by studying the changes in pressure produced on injection of various quantities of the polypeptide solution under the film. 2. The effect of 2m- and 4m-urea on the character of the adsorption is reported. 3. Structures for the adsorbed films formed in this way are suggested. 4. If the lipid monolayer is taken as a suitable model of cell membranes, then it may be supposed that the effect of such structures forming in cell membranes would be to provide effective ‘pores’ to facilitate the movement of water and other small molecules across the membrane.

scholarly journals Direct in situ measurement of specific capacitance, monolayer tension, and bilayer tension in a droplet interface bilayer

Soft Matter ◽  
2015 ◽  
Vol 11 (38) ◽  
pp. 7592-7605 ◽  
Author(s):  
Graham J. Taylor ◽  
Guru A. Venkatesan ◽  
C. Patrick Collier ◽  
Stephen A. Sarles
Keyword(s):  
Specific Capacitance ◽  
Cell Membranes ◽  
In Situ Measurement ◽  
Physical Parameters ◽  
Model Cell ◽  
Droplet Interface Bilayer

Thickness and tension are important physical parameters of model cell membranes.

The role of temperature in the formation of human–mimetic artificial cell membranes using droplet interface bilayers (DIBs)

Soft Matter ◽  
2021 ◽  
Vol 17 (39) ◽  
pp. 8891-8901
Author(s):  
Jaime L. Korner ◽  
Katherine S. Elvira
Keyword(s):  
Systematic Study ◽  
Cell Membranes ◽  
Artificial Cell ◽  
Droplet Interface Bilayer

A systematic study of the role of temperature in human–mimetic droplet interface bilayer (DIB) formation.

Photoreceptor disk membrane substructures by means of the complementary freeze-fracture-replica methods

1978 ◽  
Vol 36 (2) ◽  
pp. 156-157
Author(s):  
A. Tonosaki ◽  
M. Yamasaki ◽  
H. Washioka ◽  
J. Mizoguchi
Keyword(s):  
Cell Membranes ◽  
Freeze Fracture ◽  
Purple Membrane ◽  
Remarkable Case ◽  
Single Face ◽  
Disk Membrane ◽  
Associated Proteins ◽  
Photoreceptor Membranes

A vertebrate disk membrane is composed of 40 % lipids and 60 % proteins. Its fracture faces have been classed into the plasmic (PF) and exoplasmic faces (EF), complementary with each other, like those of most other types of cell membranes. The hypothesis assuming the PF particles as representing membrane-associated proteins has been challenged by serious questions if they in fact emerge from the crystalline formation or decoration effects during freezing and shadowing processes. This problem seems to be yet unanswered, despite the remarkable case of the purple membrane of Halobacterium, partly because most observations have been made on the replicas from a single face of specimen, and partly because, in the case of photoreceptor membranes, the conformation of a rhodopsin and its relatives remains yet uncertain. The former defect seems to be partially fulfilled with complementary replica methods.

Introduction to Cell Membranes at Molecular Resolution

1978 ◽  
Vol 36 (3) ◽  
pp. 497-502
Author(s):  
R.J. Barrnett
Keyword(s):  
Cell Membranes ◽  
International Federation ◽  
Mountain Range ◽  
Cellular Membranes ◽  
Short History ◽  
Biological Organization ◽  
Macromolecular Assemblies ◽  
The Past

This subject, is like observing the panorama of a mountain range, magnificent towering peaks, but it doesn't take much duration of observation to recognize that they are still in the process of formation. The mountains consist of approaches, materials and methods and the rocky substance of information has accumulated to such a degree that I find myself concentrating on the foothills in the foreground in order to keep up with the advance; the edifices behind form a wonderous, substantive background. It's a short history for such an accumulation and much of it has been moved by the members of the societies that make up this International Federation. My panel of speakers are here to provide what we hope is an interesting scientific fare, based on the fact that there is a continuum of biological organization from biochemical molecules through macromolecular assemblies and cellular membranes to the cell itself. Indeed, this fact explains the whole range of towering peaks that have emerged progressively during the past 25 years.

Gap junctions in the prothoracic glands of the tobacco hornworm, Manduca sexta

1992 ◽  
Vol 50 (1) ◽  
pp. 706-707
Author(s):  
Ji-da Dai ◽  
M. Joseph Costello ◽  
Lawrence I. Gilbert
Keyword(s):  
Gap Junctions ◽  
Small Molecules ◽  
Manduca Sexta ◽  
Freeze Fracture ◽  
Cell Communication ◽  
Cellular Level ◽  
Thin Sections ◽  
Prothoracic Glands ◽  
Polyhydroxylated Steroids ◽  
Stimulation Of

Insect molting and metamorphosis are elicited by a class of polyhydroxylated steroids, ecdysteroids, that originate in the prothoracic glands (PGs). Prothoracicotropic hormone stimulation of steroidogenesis by the PGs at the cellular level involves both calcium and cAMP. Cell-to-cell communication mediated by gap junctions may play a key role in regulating signal transduction by controlling the transmission of small molecules and ions between adjacent cells. This is the first report of gap junctions in the PGs, the evidence obtained by means of SEM, thin sections and freeze-fracture replicas.

Fusion and Fission Processes in Exocytosis: Possible Roles for Synexin and Osmotic Lysis in the Two Events

1980 ◽  
Vol 38 ◽  
pp. 594-597
Author(s):  
H.B. Pollard ◽  
C.E. Creutz ◽  
C.J. Pazoles ◽  
J.H. Scott
Keyword(s):  
Small Molecules ◽  
Chromaffin Cells ◽  
Adrenal Glands ◽  
General Concept ◽  
Extracellular Calcium ◽  
Large Protein ◽  
Granule Membrane ◽  
Osmotic Lysis ◽  
Per Se ◽  
Chemical Evidence

Exocytosis is a general concept describing secretion of enzymes, hormones and transmitters that are otherwise sequestered in intracellular granules. Chemical evidence for this concept was first gathered from studies on chromaffin cells in perfused adrenal glands, in which it was found that granule contents, including both large protein and small molecules such as adrenaline and ATP, were released together while the granule membrane was retained in the cell. A number of exhaustive reviews of this early work have been published and are summarized in Reference 1. The critical experiments demonstrating the importance of extracellular calcium for exocytosis per se were also first performed in this system (2,3), further indicating the substantial service given by chromaffin cells to those interested in secretory phenomena over the years.

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