scholarly journals Direct in situ measurement of specific capacitance, monolayer tension, and bilayer tension in a droplet interface bilayer

Soft Matter ◽  
2015 ◽  
Vol 11 (38) ◽  
pp. 7592-7605 ◽  
Author(s):  
Graham J. Taylor ◽  
Guru A. Venkatesan ◽  
C. Patrick Collier ◽  
Stephen A. Sarles
Keyword(s):  
Specific Capacitance ◽  
Cell Membranes ◽  
In Situ Measurement ◽  
Physical Parameters ◽  
Model Cell ◽  
Droplet Interface Bilayer

Thickness and tension are important physical parameters of model cell membranes.

scholarly journals Exploring the Interactions of Ruthenium (II) Carbosilane Metallodendrimers and Precursors with Model Cell Membranes through a Dual Spin-Label Spin-Probe Technique Using EPR

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 540 ◽  
Author(s):  
Riccardo Carloni ◽  
Natalia Sanz del Olmo ◽  
Paula Ortega ◽  
Alberto Fattori ◽  
Rafael Gómez ◽  
...  
Keyword(s):  
Spin Label ◽  
Spin Probe ◽  
Cell Membranes ◽  
Antitumor Agents ◽  
Hydrophobic Interactions ◽  
High Surface ◽  
High Surface Area ◽  
Surface Groups ◽  
Model Cell

Dendrimers exhibit unique interactions with cell membranes, arising from their nanometric size and high surface area. To a great extent, these interactions define their biological activity and can be reported in situ by spin-labelling techniques. Schiff-base carbosilane ruthenium (II) metallodendrimers are promising antitumor agents with a mechanism of action yet to explore. In order to study their in situ interactions with model cell membranes occurring at a molecular level, namely cetyltrimethylammonium bromide micelles (CTAB) and lecithin liposomes (LEC), electron paramagnetic resonance (EPR) was selected. Both a spin probe, 4-(N,N-dimethyl-N-dodecyl)ammonium-2,2,6,6-tetramethylpiperidine-1-oxyl bromide (CAT12), able to enter the model membranes, and a spin label, 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) covalently attached at newly synthesized heterofunctional dendrimers, were used to provide complementary information on the dendrimer–membrane interactions. The computer-aided EPR analysis demonstrated a good agreement between the results obtained for the spin probe and spin label experiments. Both points of view suggested the partial insertion of the dendrimer surface groups into the surfactant aggregates, mainly CTAB micelles, and the occurrence of both polar and hydrophobic interactions, while dendrimer–LEC interactions involved more polar interactions between surface groups. We found out that subtle changes in the dendrimer structure greatly modified their interacting abilities and, subsequently, their anticancer activity.

In Situ Measurement of Soil Heat Flux with the Gradient Method

2003 ◽  
Vol 2 (4) ◽  
pp. 589
Author(s):  
Douglas R. Cobos ◽  
John M. Baker
Keyword(s):  
Heat Flux ◽  
Gradient Method ◽  
Soil Heat Flux ◽  
In Situ Measurement

In-Situ Measurement Technique of Stress and Structure Change

2015 ◽  
Vol 84 (8) ◽  
pp. 567-572
Author(s):  
Tadafumi HASHIMOTO ◽  
Masahito MOCHIZUKI
Keyword(s):  
Measurement Technique ◽  
Structure Change ◽  
In Situ Measurement
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