scholarly journals CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

2021 ◽  
Author(s):  
Wei Feng ◽  
Ashley M. Newbigging ◽  
Jeffrey Tao ◽  
Yiren Cao ◽  
Hanyong Peng ◽  
...  
Keyword(s):  
Analytical Chemistry ◽  
Nucleic Acids ◽  
Small Molecules ◽  
Genome Engineering ◽  
Molecular Assays ◽  
Diagnostic Technology

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) protein systems revolutionize genome engineering and advance analytical chemistry and diagnostic technology.

Structural Insights into the Recognition of DNA Defects by Small Molecules

2021 ◽  
Author(s):  
David Dayanidhi Paul Elisa Sundar ◽  
Vaidyanathan Ganesan
Keyword(s):  
Nucleic Acids ◽  
Metal Complexes ◽  
Small Molecules ◽  
Chemical Properties ◽  
Biological Applications ◽  
Binding Interaction ◽  
Structural Insights

Studies on the binding interaction of small molecules and nucleic acids have been explored for their biological applications. With excellent photophysical/chemical properties, numerous metal complexes have been studied as structural...

scholarly journals Fluorinated molecular beacons as functional DNA nanomolecules for cellular imaging

2017 ◽  
Vol 8 (10) ◽  
pp. 7082-7086 ◽  
Author(s):  
Cheng Jin ◽  
Ting Fu ◽  
Ruowen Wang ◽  
Hui Liu ◽  
Jianmei Zou ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Small Molecules ◽  
Cellular Imaging ◽  
Molecular Beacons ◽  
Functional Dna

Molecular beacons (MBs) are simple, but practical, fluorescent nanoprobes widely used to detect small molecules, nucleic acids and proteins.

scholarly journals The energetics of small molecules binding with nucleic acids

2019 ◽  
Vol 139 ◽  
pp. 105887
Author(s):  
Viktor V. Kostjukov ◽  
Maxim P. Evstigneev
Keyword(s):  
Nucleic Acids ◽  
Small Molecules

scholarly journals Molecular assays for the detection of prostate tumor derived nucleic acids in peripheral blood

2010 ◽  
Vol 9 (1) ◽  
pp. 174 ◽  
Author(s):  
Matthias Jost ◽  
John R Day ◽  
Ryan Slaughter ◽  
Theodore D Koreckij ◽  
Deanna Gonzales ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Peripheral Blood ◽  
Prostate Tumor ◽  
Molecular Assays

scholarly journals Carbon-based dots for electrochemiluminescence sensing

2020 ◽  
Vol 4 (2) ◽  
pp. 369-385 ◽  
Author(s):  
Ying Chen ◽  
Yue Cao ◽  
Cheng Ma ◽  
Jun-Jie Zhu
Keyword(s):  
Nucleic Acids ◽  
Metal Ions ◽  
Small Molecules ◽  
Analytical Approaches ◽  
Carbon Based

This review summarizes the recent development of ECL sensors based on carbon-based dots. Particularly, various analytical approaches involving metal ions, small molecules, proteins, nucleic acids and cells are thoroughly presented.

scholarly journals How ‘Protein-Docking’ Translates into the New Emerging Field of Docking Small Molecules to Nucleic Acids?

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2749
Author(s):  
Francesca Tessaro ◽  
Leonardo Scapozza
Keyword(s):  
Molecular Docking ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Small Molecules ◽  
Viral Infections ◽  
Therapeutic Potential ◽  
Mrna Level ◽  
Protein Docking ◽  
Structural Nature ◽  
Rigid Docking

In this review, we retraced the ‘40-year evolution’ of molecular docking algorithms. Over the course of the years, their development allowed to progress from the so-called ‘rigid-docking’ searching methods to the more sophisticated ‘semi-flexible’ and ‘flexible docking’ algorithms. Together with the advancement of computing architecture and power, molecular docking’s applications also exponentially increased, from a single-ligand binding calculation to large screening and polypharmacology profiles. Recently targeting nucleic acids with small molecules has emerged as a valuable therapeutic strategy especially for cancer treatment, along with bacterial and viral infections. For example, therapeutic intervention at the mRNA level allows to overcome the problematic of undruggable proteins without modifying the genome. Despite the promising therapeutic potential of nucleic acids, molecular docking programs have been optimized mostly for proteins. Here, we have analyzed literature data on nucleic acid to benchmark some of the widely used docking programs. Finally, the comparison between proteins and nucleic acid targets docking highlighted similarity and differences, which are intrinsically related to their chemical and structural nature.

Target-directed enzyme-free dual-amplification DNA circuit for rapid signal amplification

2020 ◽  
Vol 8 (47) ◽  
pp. 10770-10775
Author(s):  
Chao Xing ◽  
Ziyi Chen ◽  
Cheng Zhang ◽  
Jun Wang ◽  
Chunhua Lu
Keyword(s):  
Nucleic Acids ◽  
Small Molecules ◽  
Signal Amplification ◽  
Fluorescence Analysis ◽  
Single Step ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Dual Amplification

An enzyme-free, single-step and rapid signal amplification DNA circuit was developed by integrating target-directed entropy-driven catalysis and hybridization chain reaction for fluorescence analysis of nucleic acids and small molecules.

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