scholarly journals Self-assembled amphiphilic fluorescent probe: detecting pH-fluctuations within cancer cells and tumour tissues

2020 ◽  
Vol 11 (36) ◽  
pp. 9875-9883 ◽  
Author(s):  
Soo Yeon Kim ◽  
Arup Podder ◽  
Hyunseung Lee ◽  
Youn-Joo Cho ◽  
Eun Hee Han ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probe ◽  
Self Assembled ◽  
Ph Fluctuations

A self-assembled amphiphilic fluorescent probe allows pH-fluctuations within cancer cells and tumour tissues to be readily detected.

Amphiphilic fluorescent probe self-encored in plasma to detect pH fluctuations in cancer cell membranes

2021 ◽  
Author(s):  
Arup Podder ◽  
Manu M. Joseph ◽  
Shayeri Biswas ◽  
Sanjib Samanta ◽  
Kaustabh K. Maiti ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Cancer Cell ◽  
Cell Membranes ◽  
Normal Cells ◽  
Turn On ◽  
Ph Fluctuations

Newly developed an amphiphilic “turn-on” fluorescent probe (P1CS) enables to distinguish of cancer cells from normal cells through mapping of pH fluctuations in cell-surface.

An AIE-based self-assembled fluorescent probe for COX-2 imaging

2019 ◽  
Vol 43 (20) ◽  
pp. 7874-7880 ◽  
Author(s):  
Xiaozheng Cao ◽  
Tang Gao ◽  
Jie Dong ◽  
Xinchen Jiang ◽  
Hui Zou ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probe ◽  
Cyclooxygenase 2 ◽  
Normal Cells ◽  
Cox 2 ◽  
Self Assembled

The first AIE-based fluorescent probe TPI-IMC was developed for imaging of cyclooxygenase-2 (COX-2) in normal cells and cancer cells.

Synthesis and Characterization of ROSA Dye - A Rhodamine B-type Fluorophore, Suitable for Bioconjugation and Fluorescence Studies in Live Cells

2019 ◽  
Vol 26 (10) ◽  
pp. 758-767
Author(s):  
Vicente Rubio ◽  
Vijaya Iragavarapu ◽  
Maciej J. Stawikowski
Keyword(s):  
Quantum Yield ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Extinction Coefficient ◽  
Rhodamine B ◽  
Solid Phase ◽  
Total Yield ◽  
Molar Extinction Coefficient ◽  
Αvβ3 Integrin ◽  
Αvβ3 Integrins

Background: Herein we report the multigram-scale synthesis, characterization and application of a rhodamine B-based fluorophore (ROSA) suitable for fluorescent studies in biological applications. This fluorophore is devoid of rhodamine spirolactone formation and furthermore characterized by a high molar extinction coefficient (ϵ=87250 ± 1630 M-1cm-1) and quantum yield (φ) of 0.589 ± 0.070 in water. Reported here is also the application of ROSA towards synthesis of a ROSA-PEG-GRGDS-NH2 fluorescent probe suitable for live cell imaging of αvβ3 integrins for in vitro assays. Objective: The main objective of this study is to efficiently prepare rhodamine B derivative, devoid of spirolactone formation that would be suitable for bioconjugation and subsequent bioimaging. Methods: Rhodamine B was transformed into rhodamine B succinimide ester (RhoB-OSu) using N-hydroxysuccinimide. RhoB-OSu was further coupled to sarcosine to obtain rhodamine Bsarcosine dye (ROSA) in good yield. The ROSA dye was then coupled to a αvβ3 integrin binding sequence using standard solid-phase conditions. Resulting ROSA-PEG-GRGDS-NH2 probe was used to image integrins on cancer cells. Results: The rhodamine B-sarcosine dye (ROSA) was obtained in multigram scale in good total yield of 47%. Unlike rhodamine B, the ROSA dye does not undergo pH-dependent spirolactone/spirolactam formation as compared with rhodamine B-glycine. It is also characterized by excellent quantum yield (φ) of 0.589 ± 0.070 in water and high molar extinction coefficient of 87250 ± 1630 M-1cm-1. ROSA coupling to the RGD-like peptide was proved to be efficient and straightforward. Imaging using standard filters on multimode plate reader and confocal microscope was performed. The αvβ3 integrins present on the surface of live WM-266-4 (melanoma) and MCF- 7 (breast cancer) cells were successfully imaged. Conclusion: We successfully derivatized rhodamine B to create an inexpensive, stable and convenient to use fluorescent probe. The obtained derivative has excellent photochemical properties and it is suitable for bioconjugation and many imaging applications.

Tracking lipid droplet dynamics for the discrimination of cancer cells by a solvatochromic fluorescent probe

2021 ◽  
Vol 333 ◽  
pp. 129541
Author(s):  
Linlin Yang ◽  
Jianping Wang ◽  
Bianhua Liu ◽  
Guangmei Han ◽  
Hong Wang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probe ◽  
Lipid Droplet ◽  
Droplet Dynamics

Self-Assembled ZnO Nanoparticle Capsules for Carrying and Delivering Isotretinoin to Cancer Cells

2017 ◽  
Vol 9 (22) ◽  
pp. 18474-18481 ◽  
Author(s):  
Wei Zhao ◽  
Ji-Shi Wei ◽  
Peng Zhang ◽  
Jie Chen ◽  
Ji-Lie Kong ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Zno Nanoparticle ◽  
Self Assembled

An “Enhanced PET”-Based Fluorescent Probe with Ultrasensitivity for Imaging Basal and Elesclomol-Induced HClO in Cancer Cells

2014 ◽  
Vol 136 (37) ◽  
pp. 12820-12823 ◽  
Author(s):  
Hao Zhu ◽  
Jiangli Fan ◽  
Jingyun Wang ◽  
Huiying Mu ◽  
Xiaojun Peng
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probe

Self-assembled vesicle and rod-like aggregates of functionalized perylene diimide: reaction-based near-IR intracellular fluorescent probe for selective detection of palladium

2016 ◽  
Vol 4 (21) ◽  
pp. 3750-3759 ◽  
Author(s):  
Prabhpreet Singh ◽  
Lalit Singh Mittal ◽  
Vanita Vanita ◽  
Kapil Kumar ◽  
Amandeep Walia ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hela Cells ◽  
Perylene Diimide ◽  
Selective Detection ◽  
Near Ir ◽  
Absorbance Changes ◽  
Self Assembled ◽  
Ratiometric Absorbance

Aggregates ofPS-PDIafter Pd0based depropargylation show de-aggregation and near-IR, ratiometric absorbance changes in water and live HeLa cells.

A self-assembled π-conjugated system as an anti-proliferative agent in prostate cancer cells and a probe for intra-cellular imaging

RSC Advances ◽  
2014 ◽  
Vol 4 (89) ◽  
pp. 48433-48437 ◽  
Author(s):  
Krishnamoorthy Lalitha ◽  
Preethi Jenifer ◽  
Y. Siva Prasad ◽  
Kumarasamy Muthusamy ◽  
George John ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Renewable Resource ◽  
Cellular Imaging ◽  
Prostate Cancer Cells ◽  
Conjugated Systems ◽  
Pc3 Cells ◽  
Self Assembled

Herein, self-assembled π-conjugated systems derived from renewable resource are reported as a probe for intra-cellular imaging and an anti-proliferative agent for PC3 cells.

Glucose oxidase mediated targeted cancer-starving therapy by biotinylated self-assembled vesicles

2018 ◽  
Vol 54 (71) ◽  
pp. 9929-9932 ◽  
Author(s):  
Soumik Dinda ◽  
Saheli Sarkar ◽  
Prasanta Kumar Das
Keyword(s):  
Glucose Oxidase ◽  
Cancer Cells ◽  
Energy Supply ◽  
Normal Cells ◽  
Trimesic Acid ◽  
Self Assembled

Glucose oxidase (GOx) mediated targeted cancer-starving therapy, by blocking the energy supply to cancer cells, has been demonstrated using GOx encapsulating monolayer vesicles of a trimesic acid based biotinylated amphiphile (TMB). GOx, loaded within the TMB vesicles, was selectively delivered inside the cancer cells, resulting in ∼6-fold higher killing of cancer cells compared to normal cells.

Sign in / Sign up

Export Citation Format

Share Document