scholarly journals Fucosylated ubiquitin and orthogonally glycosylated mutant A28C: conceptually new ligands for Burkholderia ambifaria lectin (BambL)

2020 ◽  
Vol 11 (47) ◽  
pp. 12662-12670
Author(s):  
Sakonwan Kuhaudomlarp ◽  
Linda Cerofolini ◽  
Sabrina Santarsia ◽  
Emilie Gillon ◽  
Silvia Fallarini ◽  
...  
Keyword(s):  
Protein Scaffolds ◽  
Click Reactions ◽  
Metal Free ◽  
High Affinity ◽  
Burkholderia Ambifaria

Metal free click reactions used to glycosylate ubiquitin and its mutant A28C afforded two protein scaffolds with high affinity for Burkholderia ambifaria lectin (BambL).

scholarly journals Protein Scaffolds for Multivalent Display of High‐Affinity CD22 Ligands

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Mary K. O’Reilly ◽  
Liang Liao ◽  
Cory Rillahan ◽  
Brian E. Collins ◽  
Shoufa Han ◽  
...  
Keyword(s):  
Protein Scaffolds ◽  
High Affinity ◽  
Multivalent Display

External electric field control: driving the reactivity of metal-free azide–alkyne click reactions

2017 ◽  
Vol 19 (33) ◽  
pp. 22482-22486 ◽  
Author(s):  
Kalishankar Bhattacharyya ◽  
Sharmistha Karmakar ◽  
Ayan Datta
Keyword(s):  
Electric Field ◽  
External Electric Field ◽  
Efficient Catalyst ◽  
Click Reactions ◽  
Metal Free ◽  
Field Control

An external electric field is demonstrated as an efficient catalyst to accelerate click reactions.

Three-Fold Metal-Free Efficient (“Click”) Reactions onto a Multifunctional Poly(2-oxazoline) Designer Scaffold

2011 ◽  
Vol 44 (16) ◽  
pp. 6424-6432 ◽  
Author(s):  
Kristian Kempe ◽  
Richard Hoogenboom ◽  
Michael Jaeger ◽  
Ulrich S. Schubert
Keyword(s):  
Click Reactions ◽  
Metal Free

(Z)-Selective anti-Markovnikov or Markovnikov thiol–yne-click reactions of an internal alkyne by amide hydrogen bond control

2020 ◽  
Vol 56 (20) ◽  
pp. 2991-2994 ◽  
Author(s):  
Milan Pramanik ◽  
Khokan Choudhuri ◽  
Subhayan Chakraborty ◽  
Arindam Ghosh ◽  
Prasenjit Mal
Keyword(s):  
Hydrogen Bond ◽  
Hydrogen Bonding ◽  
Amide Hydrogen ◽  
Click Reactions ◽  
Metal Free ◽  
Hydrogen Bonding Interactions ◽  
Internal Alkyne

Hydrogen bonding interactions like N–H⋯S, N–H⋯N and C–H⋯O helped to obtain either (Z)-selective anti-Markovnikov or Markovnikov Thiol–Yne-Click products exclusively with excellent yields from an internal alkyne under metal free conditions.

All in one: The preparation of polyester/silica hybrid nanocomposites via three different metal-free click reactions

2021 ◽  
pp. 110532
Author(s):  
Ozgun Daglar ◽  
Emrah Çakmakçi ◽  
Ufuk Saim Gunay ◽  
Gurkan Hizal ◽  
Umit Tunca ◽  
...  
Keyword(s):  
Hybrid Nanocomposites ◽  
Click Reactions ◽  
Metal Free ◽  
Silica Hybrid

Novel, Metal-Free, Superacid-Catalyzed “Click” Reactions of Isatins with Linear, Nonactivated, Multiring Aromatic Hydrocarbons

2010 ◽  
Vol 43 (17) ◽  
pp. 6968-6979 ◽  
Author(s):  
M. Carmen G. Hernandez ◽  
Mikhail G. Zolotukhin ◽  
Serguei Fomine ◽  
Gerardo Cedillo ◽  
Salvador L. Morales ◽  
...  
Keyword(s):  
Aromatic Hydrocarbons ◽  
Click Reactions ◽  
Metal Free

scholarly journals Fucosylated Ubiquitin and Orthogonally Glycosylated Mutant A28C: Conceptually New Ligands for Burkholderia Ambifaria Lectin (BambL)†

2020 ◽  
Author(s):  
Cristina Nativi ◽  
Sakonwan Kuhaudomlarp ◽  
Linda Cerofolini ◽  
Sabrina Santarsia ◽  
Emilie Gillon ◽  
...  
Keyword(s):  
High Affinity ◽  
Burkholderia Ambifaria

Ubiquitin as <i>scaffold</i> <i>protein</i> and aryl-α-O-fucoside as determinant to achieve conceptually new ligands with high affinity for <i>Burkholderia ambifaria </i>lectin are described.<div>Ub mutant A28C which displays a Cys residue in addition to the eight Lys residues was expressed. </div><div>The resulting <i>scaffold</i> was orthogonally glycosylated </div><div>to display one thio-rhamnose and up to eight residues of aryl-α-O-fucoside. </div><div>The glycosylated Ub-based scaffolds have unprecedented immune properties.<br></div>

scholarly journals A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization

Research ◽  
2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Xianglong Hu ◽  
Xueqian Zhao ◽  
Benzhao He ◽  
Zheng Zhao ◽  
Zheng Zheng ◽  
...  
Keyword(s):  
Fluorescence Labeling ◽  
Hydroxyl Groups ◽  
Metal Catalysis ◽  
Click Reactions ◽  
Metal Free ◽  
Gram Positive Bacteria ◽  
Natural Polysaccharide ◽  
High Efficient ◽  
Activated Alkynes

The efficient bioconjugation of functional groups/molecules to targeted matrix and bio-related species drives the great development of material science and biomedicine, while the dilemma of metal catalysis, uneasy premodification, and limited reaction efficiency in traditional bioconjugation has restricted the booming development to some extent. Here, we provide a strategy for metal-free click bioconjugation at diverse levels based on activated alkynes. As a proof-of-concept, the abundant native groups including amine, thiol, and hydroxyl groups can directly react with activated alkynes without any modification in the absence of metal catalysis. Through this strategy, high-efficient modification and potential functionalization can be achieved for natural polysaccharide, biocompatible polyethylene glycol (PEG), synthetic polymers, cell penetrating peptide, protein, fast whole-cell mapping, and even quick differentiation and staining of Gram-positive bacteria, etc. Therefore, current metal-free click bioconjugation strategy based on activated alkynes is promising for the development of quick fluorescence labeling and functional modification of many targets and can be widely applied towards the fabrication of complex biomaterials and future in vivo labeling and detection.

Environmentally Benign Large-Scale Synthesis of a Precursor to Vortioxetine

Synthesis ◽  
2020 ◽  
Vol 52 (18) ◽  
pp. 2662-2666
Author(s):  
John K. Gallos ◽  
Stavroula A. Zisopoulou ◽  
Anastasia E. Pafili ◽  
Petros Gkizis ◽  
Thanos Andreou ◽  
...  
Keyword(s):  
Major Depressive Disorder ◽  
Transition Metal ◽  
Depressive Disorder ◽  
Large Scale ◽  
Choline Chloride ◽  
Deep Eutectic Solvents ◽  
Environmentally Benign ◽  
Major Depressive ◽  
Metal Free ◽  
High Affinity

An eco-friendly, high-yielding, and transition-metal-free synthesis of 2-[(2,4-dimethylphenyl)thio]aniline precursor to vortioxetine is reported. Vortioxetine, a multi-modal acting drug with high affinity for a range of serotonergic targets, is used for the treatment of major depressive disorder (MDD). The synthesis – applicable in multi-gram scale – involves the reaction of bis(2,4-dimethyl)iodonium bromide with commercial 2-aminophenyl disulfide, whereas its reaction with 2-aminothiophenol afforded the same product but in low to moderate yields. This method works equally well in deep eutectic solvents (DESs), based on choline chloride (ChCl).

Sign in / Sign up

Export Citation Format

Share Document