scholarly journals A new approach based on isoindole formation reaction for sensitive fluorimetric assay of milnacipran in tablets and biological fluids (plasma/urine)

RSC Advances ◽  
2020 ◽  
Vol 10 (64) ◽  
pp. 38884-38889
Author(s):  
Ahmed A. Abu-hassan ◽  
Ramadan Ali ◽  
Sayed M. Derayea
Keyword(s):  
Biological Fluids ◽  
New Approach ◽  
Formation Reaction ◽  
Fluorimetric Assay

The current study describes a new, sensitive, and economic protocol for milnacipran analysis.

An "antibody electrode," preliminary report on a new approach in enzyme immunoassay.

1979 ◽  
Vol 25 (2) ◽  
pp. 318-321 ◽  
Author(s):  
J L Boitieux ◽  
G Desmet ◽  
D Thomas
Keyword(s):  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Solid Phase ◽  
Biological Fluids ◽  
Hepatitis B Surface Antigen ◽  
Antigen Concentration ◽  
New Approach ◽  
Solid Phase Radioimmunoassay ◽  
Reliable Procedure ◽  
Sensitive Electrode

Abstract We report preliminary experiments on a new, sensitive, and reliable procedure for enzyme immunoassay of various antigens in biological fluids. The method, developed from the biological model "hepatitis B surface-antigen/antibodies," is less time consuming than most immunochemical techniques and eliminates many inconveniences arising from use of isotopes. We use a solid-phase "sandwich" procedure, the antibodies being immobilized on gelatin membranes, and determine antigen concentration with the help of an iodide-sensitive electrode modified by fixing the active membrane onto the crystal sensor. We have established the analytical criteria of the method and compared it with the solid-phase radioimmunoassay for the surface antigen in dilution series. One-tenth microgram of antigen per liter can be reproducibly detected with our method. Use of antibody electrode should easily be extended to assay of other antigens and haptens that usually are determined by radioimmunoassay.

scholarly journals Exosomes in disease and regeneration: biological functions, diagnostics, and beneficial effects

2020 ◽  
Vol 319 (6) ◽  
pp. H1162-H1180
Author(s):  
Yun Lin ◽  
Johnathon D. Anderson ◽  
Lily M. A. Rahnama ◽  
Shenwen V. Gu ◽  
Anne A. Knowlton
Keyword(s):  
Biological Fluids ◽  
Cell Types ◽  
Parent Cell ◽  
Biological Functions ◽  
Diagnostic Biomarkers ◽  
New Approach ◽  
Beneficial Effects ◽  
Isolation And Characterization ◽  
Wide Range ◽  
Specific Proteins

Exosomes are a subtype of extracellular vesicles. They range from 30 to 150 nm in diameter and originate from intraluminal vesicles. Exosomes were first identified as the mechanism for releasing unnecessary molecules from reticulocytes as they matured to red blood cells. Since then, exosomes have been shown to be secreted by a broad spectrum of cells and play an important role in the cardiovascular system. Different stimuli are associated with increased exosome release and result in different exosome content. The release of harmful DNA and other molecules via exosomes has been proposed as a mechanism to maintain cellular homeostasis. Because exosomes contain parent cell-specific proteins on the membrane and in the cargo that is delivered to recipient cells, exosomes are potential diagnostic biomarkers of various types of diseases, including cardiovascular disease. As exosomes are readily taken up by other cells, stem cell-derived exosomes have been recognized as a potential cell-free regenerative therapy to repair not only the injured heart but other tissues as well. The objective of this review is to provide an overview of the biological functions of exosomes in heart disease and tissue regeneration. Therefore, state-of-the-art methods for exosome isolation and characterization, as well as approaches to assess exosome functional properties, are reviewed. Investigation of exosomes provides a new approach to the study of disease and biological processes. Exosomes provide a potential “liquid biopsy,” as they are present in most, if not all, biological fluids that are released by a wide range of cell types.

The O–C diagrams of minor planets − a new approach to modelling the rotation

1999 ◽  
Vol 173 ◽  
pp. 185-188
Author(s):  
Gy. Szabó ◽  
K. Sárneczky ◽  
L.L. Kiss
Keyword(s):  
Error Analysis ◽  
Time Dependence ◽  
Theoretical Work ◽  
Minor Planet ◽  
Minor Planets ◽  
New Approach ◽  
Preliminary Results ◽  
Ccd Observations

AbstractA widely used tool in studying quasi-monoperiodic processes is the O–C diagram. This paper deals with the application of this diagram in minor planet studies. The main difference between our approach and the classical O–C diagram is that we transform the epoch (=time) dependence into the geocentric longitude domain. We outline a rotation modelling using this modified O–C and illustrate the abilities with detailed error analysis. The primary assumption, that the monotonity and the shape of this diagram is (almost) independent of the geometry of the asteroids is discussed and tested. The monotonity enables an unambiguous distinction between the prograde and retrograde rotation, thus the four-fold (or in some cases the two-fold) ambiguities can be avoided. This turned out to be the main advantage of the O–C examination. As an extension to the theoretical work, we present some preliminary results on 1727 Mette based on new CCD observations.

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