scholarly journals The influence of structural gradients in large pore organosilica materials on the capabilities for hosting cellular communities

RSC Advances ◽  
2020 ◽  
Vol 10 (29) ◽  
pp. 17327-17335
Author(s):  
Hannah Bronner ◽  
Anna-Katharina Holzer ◽  
Alexander Finke ◽  
Marius Kunkel ◽  
Andreas Marx ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Polymer Nanocomposites ◽  
Control Cell ◽  
Adhesion Properties ◽  
Large Pore ◽  
Organosilica Materials ◽  
Community Systems

Chemical and structural gradients in biofunctionalized organosilica–polymer nanocomposites control cell adhesion properties and open perspectives for artificial cellular community systems.

scholarly journals A simplified approach to control cell adherence on biologically derived in vitro cell culture scaffolds by direct UV-mediated RGD linkage

2020 ◽  
Vol 31 (10) ◽  
Author(s):  
A. M. Porras Hernández ◽  
H. Pohlit ◽  
F. Sjögren ◽  
L. Shi ◽  
D. Ossipov ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Biology ◽  
Reaction Pathway ◽  
Control Cell ◽  
Peptide Sequence ◽  
Good Resolution ◽  
Adhesion Properties ◽  
Simplified Approach ◽  
Mouse Brain Endothelial Cells

Abstract In this work, we present a method to fabricate a hyaluronic acid (HA) hydrogel with spatially controlled cell-adhesion properties based on photo-polymerisation cross-linking and functionalization. The approach utilises the same reaction pathway for both steps meaning that it is user-friendly and allows for adaptation at any stage during the fabrication process. Moreover, the process does not require any additional cross-linkers. The hydrogel is formed by UV-initiated radical addition reaction between acrylamide (Am) groups on the HA backbone. Cell adhesion is modulated by functionalising the adhesion peptide sequence arginine–glycine–aspartate onto the hydrogel surface via radical mediated thiol–ene reaction using the non-reacted Am groups. We show that 10 × 10 µm2 squares could be patterned with sharp features and a good resolution. The smallest area that could be patterned resulting in good cell adhesion was 25 × 25 µm2 squares, showing single-cell adhesion. Mouse brain endothelial cells adhered and remained in culture for up to 7 days on 100 × 100 µm2 square patterns. We see potential for this material combination for future use in novel organ-on-chip models and tissue engineering where the location of the cells is of importance and to further study endothelial cell biology.

scholarly journals Tailoring Pyro- and Orthophosphate Species to Enhance Stem Cell Adhesion to Phosphate Glasses

2021 ◽  
Vol 22 (2) ◽  
pp. 837
Author(s):  
Nigel De Melo ◽  
Lauren Murrell ◽  
Md Towhidul Islam ◽  
Jeremy J. Titman ◽  
Laura Macri-Pellizzeri ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Bone Repair ◽  
Therapeutic Potential ◽  
Glass Structure ◽  
Control Cell ◽  
Phosphate Glasses ◽  
Gradual Transition ◽  
Adhesion Properties ◽  
Degradation Rates

Phosphate-based glasses (PBGs) offer significant therapeutic potential due to their bioactivity, controllable compositions, and degradation rates. Several PBGs have already demonstrated their ability to support direct cell growth and in vivo cytocompatibility for bone repair applications. This study investigated development of PBG formulations with pyro- and orthophosphate species within the glass system (40 − x)P2O5·(16 + x)CaO·20Na2O·24MgO (x = 0, 5, 10 mol%) and their effect on stem cell adhesion properties. Substitution of phosphate for calcium revealed a gradual transition within the glass structure from Q2 to Q0 phosphate species. Human mesenchymal stem cells were cultured directly onto discs made from three PBG compositions. Analysis of cells seeded onto the discs revealed that PBG with higher concentration of pyro- and orthophosphate content (61% Q1 and 39% Q0) supported a 4.3-fold increase in adhered cells compared to glasses with metaphosphate connectivity (49% Q2 and 51% Q1). This study highlights that tuning the composition of PBGs to possess pyro- and orthophosphate species only, enables the possibility to control cell adhesion performance. PBGs with superior cell adhesion profiles represent ideal candidates for biomedical applications, where cell recruitment and support for tissue ingrowth are of critical importance for orthopaedic interventions.

scholarly journals Microgravity Induces Transient EMT in Human Keratinocytes by Early Down-Regulation of E-Cadherin and Cell-Adhesion Remodeling

2020 ◽  
Vol 11 (1) ◽  
pp. 110
Author(s):  
Giulia Ricci ◽  
Alessandra Cucina ◽  
Sara Proietti ◽  
Simona Dinicola ◽  
Francesca Ferranti ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Human Keratinocytes ◽  
Short Exposure ◽  
Migration And Invasion ◽  
Hacat Cells ◽  
Invasive Phenotype ◽  
Adhesion Properties ◽  
Mesenchymal Transition ◽  
Cell Matrix ◽  
E Cadherin

Changes in cell–matrix and cell-to-cell adhesion patterns are dramatically fostered by the microgravity exposure of living cells. The modification of adhesion properties could promote the emergence of a migrating and invasive phenotype. We previously demonstrated that short exposure to the simulated microgravity of human keratinocytes (HaCaT) promotes an early epithelial–mesenchymal transition (EMT). Herein, we developed this investigation to verify if the cells maintain the acquired invasive phenotype after an extended period of weightlessness exposure. We also evaluated cells’ capability in recovering epithelial characteristics when seeded again into a normal gravitational field after short microgravity exposure. We evaluated the ultra-structural junctional features of HaCaT cells by Transmission Electron Microscopy and the distribution pattern of vinculin and E-cadherin by confocal microscopy, observing a rearrangement in cell–cell and cell–matrix interactions. These results are mirrored by data provided by migration and invasion biological assay. Overall, our studies demonstrate that after extended periods of microgravity, HaCaT cells recover an epithelial phenotype by re-establishing E-cadherin-based junctions and cytoskeleton remodeling, both being instrumental in promoting a mesenchymal–epithelial transition (MET). Those findings suggest that cytoskeletal changes noticed during the first weightlessness period have a transitory character, given that they are later reversed and followed by adaptive modifications through which cells miss the acquired mesenchymal phenotype.

Redistribution and dysfunction of integrins in cultured renal epithelial cells exposed to oxidative stress

1993 ◽  
Vol 264 (1) ◽  
pp. F149-F157 ◽  
Author(s):  
J. Gailit ◽  
D. Colflesh ◽  
I. Rabiner ◽  
J. Simone ◽  
M. S. Goligorsky
Keyword(s):  
Oxidative Stress ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Surface ◽  
Apical Cell ◽  
Flow Cytometric Analysis ◽  
Adhesion Properties ◽  
Renal Tubular Cells ◽  
Renal Tubular

Tubular obstruction by detached renal tubular epithelial cells is a major cause of oliguria in acute renal failure. Viable renal tubular cells can be recovered from urine of patients with acute tubular necrosis, suggesting a possible defect in cell adhesion to the basement membrane. To study this process of epithelial cell desquamation in vitro, we investigated the effect of nonlethal oxidative stress on the integrin adhesion receptors of the primate kidney epithelial cell line BS-C-1. Morphological and functional studies of cell adhesion properties included the following: interference reflection microscopy, intravital confocal microscopy and immunocytochemistry, flow cytometric analysis of integrin receptor abundance, and cell-matrix attachment assay. High levels of the integrin subunits alpha 3, alpha v, and beta 1 were detected on the cell surface by fluorescence-activated cell sorting (FACS) analysis, as well as lower levels of alpha 1, alpha 2, alpha 4, alpha 5, alpha 6, and beta 3. Exposure of BS-C-1 cells to nonlethal oxidative stress resulted in the disruption of focal contacts, disappearance of talin from the basal cell surface, and in the redistribution of integrin alpha 3-subunits from predominantly basal location to the apical cell surface. As measured in a quantitative cell attachment assay, oxidative stress decreased BS-C-1 cell adhesion to type IV collagen, laminin, fibronectin, and vitronectin. Defective adhesion was not associated with a loss of alpha 3-, alpha 4-, or alpha v-integrin subunits from the cell surface.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals The Heparin-Binding Activity of Secreted Modular Calcium-Binding Protein 1 (SMOC-1) Modulates Its Cell Adhesion Properties

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56839 ◽  
Author(s):  
Marina Klemenčič ◽  
Marko Novinec ◽  
Silke Maier ◽  
Ursula Hartmann ◽  
Brigita Lenarčič
Keyword(s):  
Cell Adhesion ◽  
Calcium Binding ◽  
Binding Protein ◽  
Binding Activity ◽  
Calcium Binding Protein ◽  
Heparin Binding ◽  
Adhesion Properties

scholarly journals Testing the in vitro performance of hydroxyapatite coated magnesium (AZ91D) and titanium concerning cell adhesion and osteogenic differentiation

2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Claudia Kleinhans ◽  
Gabriele Vacun ◽  
Roman Surmenev ◽  
Maria Surmeneva ◽  
Petra Juliane Kluger
Keyword(s):  
Cell Adhesion ◽  
Osteogenic Differentiation ◽  
Cell Attachment ◽  
Human Mesenchymal Stem Cell ◽  
Adhesion Properties ◽  
Sputtering Deposition ◽  
Initial Cell ◽  
Tissue Culture Polystyrene ◽  
Diffraction Patterns

AbstractIn the current study the in vitro outcome of a degradable magnesium alloy (AZ91D) and standard titanium modified by nanostructured-hydroxyapatite (n-HA) coatings concerning cell adhesion and osteogenic differentiation was investigated by direct cell culture. The n-HA modification was prepared via radio-frequency magnetron sputtering deposition and proven by field emission scanning electron microscopy and X-ray powder diffraction patterns revealing a homogenous surface coating. Human mesenchymal stem cell (hMSCs) adhesion was examined after one and 14 days displaying an enhanced initial cell adhesion on the n-HA modified samples. The osteogenic lineage commitment of the cells was determined by alkaline phosphatase (ALP) quantification. On day one n-HA coated AZ91D exhibited a comparable ALP expression to standard tissue culture polystyrene samples. However, after 14 days solely little DNA and ALP amounts were measurable on n-HA coated AZ91D due to the lack of adherent cells. Titanium displayed excellent cell adhesion properties and ALP was detectable after 14 days. An increased pH of the culture was measured for AZ91D as well as for n-HA coated AZ91D. We conclude that n-HA modification improves initial cell attachment on AZ91D within the first 24 h. However, the effect does not persist for 14 days in in vitro conditions.

Controlling the Cell-Adhesion Properties of Poly(acrylic acid)/Polyacrylamide Hydrogen-Bonded Multilayers

2012 ◽  
Vol 45 (15) ◽  
pp. 6120-6126 ◽  
Author(s):  
Sang-Wook Lee ◽  
Kwadwo E. Tettey ◽  
Iris L. Kim ◽  
Jason A. Burdick ◽  
Daeyeon Lee
Keyword(s):  
Cell Adhesion ◽  
Acrylic Acid ◽  
Adhesion Properties ◽  
Poly Acrylic Acid ◽  
Hydrogen Bonded

scholarly journals Hyperbranched Polyester Hydrogels with Controlled Drug Release and Cell Adhesion Properties

2013 ◽  
Vol 14 (5) ◽  
pp. 1299-1310 ◽  
Author(s):  
Hongbin Zhang ◽  
Alpesh Patel ◽  
Akhilesh K. Gaharwar ◽  
Silvia M. Mihaila ◽  
Giorgio Iviglia ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Drug Release ◽  
Controlled Drug Release ◽  
Hyperbranched Polyester ◽  
Adhesion Properties

Development of Polymer/Nanodiamond Composite Coatings to Control Cell Adhesion, Growth, and Functions

2015 ◽  
pp. 1-26 ◽  
Author(s):  
Milena Keremidarska ◽  
Kamelia Hristova ◽  
Todor Hikov ◽  
Ekaterina Radeva ◽  
Dimitar Mitev ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Composite Coatings ◽  
Control Cell

Uncx4.1 is required for the formation of the pedicles and proximal ribs and acts upstream of Pax9

Development ◽  
2000 ◽  
Vol 127 (11) ◽  
pp. 2251-2258 ◽  
Author(s):  
A. Mansouri ◽  
A.K. Voss ◽  
T. Thomas ◽  
Y. Yokota ◽  
P. Gruss
Keyword(s):  
Cell Adhesion ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Homeobox Gene ◽  
Axial Skeleton ◽  
Adhesion Properties ◽  
Marker Analysis ◽  
Targeted Mutation ◽  
Differential Cell

The expression of the homeobox gene Uncx4.1 in the somite is restricted to the caudal half of the newly formed somite and sclerotome. Here we show that mice with a targeted mutation of the Uncx4.1 gene exhibit defects in the axial skeleton and ribs. In the absence of Uncx4.1, pedicles of the neural arches and proximal ribs are not formed. In addition, dorsal root ganglia are disorganized. Histological and marker analysis revealed that Uncx4.1 is not necessary for somite segmentation. It is required to maintain the condensation of the caudal half-sclerotome, from which the missing skeletal elements are derived. The loss of proximal ribs in Pax1/Pax9 double mutants and the data presented here argue for a role of Uncx4.1 upstream of Pax9 in the caudolateral sclerotome. Our results further indicate that Uncx4.1 may be involved in the differential cell adhesion properties of the somite.

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