An Easily Available Ratiometric AIE Probe for Nitroxyl Visualization in Vitro and in Vivo

2021 ◽  
Author(s):  
Chunbin Li ◽  
Guoyu Jiang ◽  
Xiang Liu ◽  
Qingfang Lai ◽  
Miaomiao Kang ◽  
...  
Keyword(s):  
Reactive Nitrogen Species ◽  
Generation Mechanism ◽  
Pivotal Role ◽  
Reactive Nitrogen ◽  
Nitrogen Species

Nitroxyl (HNO), as a reactive nitrogen species (RNS), has a pivotal role in many physiological and pathological processes, but the generation mechanism of endogenous HNO has not been elucidated, because...

scholarly journals Reactive Nitrogen Species Is Required for the Activation of the AMP-activated Protein Kinase by Statin in Vivo

2008 ◽  
Vol 283 (29) ◽  
pp. 20186-20197 ◽  
Author(s):  
Hyoung Chul Choi ◽  
Ping Song ◽  
Zhonglin Xie ◽  
Yong Wu ◽  
Jian Xu ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Reactive Nitrogen Species ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Amp Activated Protein Kinase

scholarly journals Withdrawal: Reactive nitrogen species is required for the activation of the AMP-activated protein kinase by statin in vivo.

2019 ◽  
Vol 294 (27) ◽  
pp. 10742-10742
Author(s):  
Hyoung Chul Choi ◽  
Ping Son ◽  
Zhonglin Xie ◽  
Yong Wu ◽  
Jian Xu ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Reactive Nitrogen Species ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Amp Activated Protein Kinase

scholarly journals Nitration of endogenous para-hydroxyphenylacetic acid and the metabolism of nitrotyrosine

2003 ◽  
Vol 374 (2) ◽  
pp. 521-527 ◽  
Author(s):  
Ali R. MANI ◽  
Ananth S. PANNALA ◽  
Nelson N. ORIE ◽  
Richard OLLOSSON ◽  
David HARRY ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Plasma Concentration ◽  
Reactive Nitrogen Species ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Integrated Index ◽  
Hydroxyphenylacetic Acid ◽  
Major Route

Reactive nitrogen species, such as peroxynitrite, can nitrate tyrosine in proteins to form nitrotyrosine. Nitrotyrosine is metabolized to 3-nitro-4-hydroxyphenylacetic acid (NHPA), which is excreted in the urine. This has led to the notion that measurement of urinary NHPA may provide a time-integrated index of nitrotyrosine formation in vivo. However, it is not known whether NHPA is derived exclusively from metabolism of nitrotyrosine, or whether it can be formed by nitration of circulating para-hydroxyphenylacetic acid (PHPA), a metabolite of tyrosine. In the present study, we have developed a gas chromatography MS assay for NHPA and PHPA to determine whether or not NHPA can be formed directly by nitration of PHPA. Following the injection of nitrotyrosine, 0.5±0.16% of injected dose was recovered unchanged as nitrotyrosine, and 4.3±0.2% as NHPA in the urine. To determine whether or not NHPA could be formed by the nitration of PHPA, deuterium-labelled PHPA ([2H6]PHPA) was injected, and the formation of deuterated NHPA ([2H5]NHPA) was measured. Of the infused [2H6]PHPA, 78±2% was recovered in the urine unchanged, and approx. 0.23% was recovered as [2H5]NHPA. Since the plasma concentration of PHPA is markedly higher than free nitrotyrosine (approx. 400-fold), the nitration of high-circulating endogenous PHPA to form NHPA becomes very significant and accounts for the majority of NHPA excreted in urine. This is the first study to demonstrate that NHPA can be formed by nitration of PHPA in vivo, and that this is the major route for its formation.

Inhibition of Reactive Nitrogen Species in Vitro and ex Vivo by Trypsin Inhibitor from Sweet Potato ‘Tainong 57' Storage Roots

2007 ◽  
Vol 55 (15) ◽  
pp. 6000-6006 ◽  
Author(s):  
Guan-Jhong Huang ◽  
Ming-Jyh Sheu ◽  
Hsien-Jung Chen ◽  
Yuan-Shiun Chang ◽  
Yaw-Huei Lin
Keyword(s):  
Sweet Potato ◽  
Trypsin Inhibitor ◽  
Reactive Nitrogen Species ◽  
Ex Vivo ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Storage Roots

scholarly journals Myeloperoxidase-generated reactive nitrogen species convert LDL into an atherogenic form in vitro

1999 ◽  
Vol 103 (11) ◽  
pp. 1547-1560 ◽  
Author(s):  
Eugene A. Podrez ◽  
David Schmitt ◽  
Henry F. Hoff ◽  
Stanley L. Hazen
Keyword(s):  
Reactive Nitrogen Species ◽  
Reactive Nitrogen ◽  
Nitrogen Species

scholarly journals Withdrawal: Activation of the AMP-activated protein kinase by the anti-diabetic drug metformin in vivo : Role of mitochondrial reactive nitrogen species.

2019 ◽  
Vol 294 (36) ◽  
pp. 13525-13525 ◽  
Author(s):  
Ming-Hui Zou ◽  
Stacy S. Kirkpatrick ◽  
Bradley J. Davis ◽  
John S. Nelson ◽  
Walger G. Wiles ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Reactive Nitrogen Species ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Amp Activated Protein Kinase

scholarly journals On the Liquid Chemistry of the Reactive Nitrogen Species Peroxynitrite and Nitrogen Dioxide Generated by Physical Plasmas

Biomolecules ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1687
Author(s):  
Giuliana Bruno ◽  
Sebastian Wenske ◽  
Jan-Wilm Lackmann ◽  
Michael Lalk ◽  
Thomas von Woedtke ◽  
...  
Keyword(s):  
Reactive Nitrogen Species ◽  
High Resolution Mass Spectrometry ◽  
Clinical Effectiveness ◽  
Covalent Modification ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Plasma Parameters ◽  
Cold Plasmas ◽  
Major Application

Cold physical plasmas modulate cellular redox signaling processes, leading to the evolution of a number of clinical applications in recent years. They are a source of small reactive species, including reactive nitrogen species (RNS). Wound healing is a major application and, as its physiology involves RNS signaling, a correlation between clinical effectiveness and the activity of plasma-derived RNS seems evident. To investigate the type and reactivity of plasma-derived RNS in aqueous systems, a model with tyrosine as a tracer was utilized. By high-resolution mass spectrometry, 26 different tyrosine derivatives including the physiologic nitrotyrosine were identified. The product pattern was distinctive in terms of plasma parameters, especially gas phase composition. By scavenger experiments and isotopic labelling, gaseous nitric dioxide radicals and liquid phase peroxynitrite ions were determined as dominant RNS. The presence of water molecules in the active plasma favored the generation of peroxynitrite. A pilot study, identifying RNS driven post-translational modifications of proteins in healing human wounds after the treatment with cold plasma (kINPen), demonstrated the presence of in vitro determined chemical pathways. The plasma-driven nitration and nitrosylation of tyrosine allows the conclusion that covalent modification of biomolecules by RNS contributes to the clinically observed impact of cold plasmas.

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