Inhibition of Reactive Nitrogen Species in Vitro and ex Vivo by Trypsin Inhibitor from Sweet Potato ‘Tainong 57' Storage Roots

2007 ◽  
Vol 55 (15) ◽  
pp. 6000-6006 ◽  
Author(s):  
Guan-Jhong Huang ◽  
Ming-Jyh Sheu ◽  
Hsien-Jung Chen ◽  
Yuan-Shiun Chang ◽  
Yaw-Huei Lin
Keyword(s):  
Sweet Potato ◽  
Trypsin Inhibitor ◽  
Reactive Nitrogen Species ◽  
Ex Vivo ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Storage Roots

Inhibition of reactive nitrogen species in vitro and ex vivo by thioredoxin h2 from sweet potato ‘Tainong 57’ storage roots

2012 ◽  
Vol 131 (2) ◽  
pp. 552-557 ◽  
Author(s):  
Guan-Jhong Huang ◽  
Jeng-Shyan Deng ◽  
Hsien-Jung Chen ◽  
Shyh-Shyun Huang ◽  
Chieh-Hsi Wu ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Reactive Nitrogen Species ◽  
Ex Vivo ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Storage Roots

scholarly journals Myeloperoxidase-generated reactive nitrogen species convert LDL into an atherogenic form in vitro

1999 ◽  
Vol 103 (11) ◽  
pp. 1547-1560 ◽  
Author(s):  
Eugene A. Podrez ◽  
David Schmitt ◽  
Henry F. Hoff ◽  
Stanley L. Hazen
Keyword(s):  
Reactive Nitrogen Species ◽  
Reactive Nitrogen ◽  
Nitrogen Species

scholarly journals On the Liquid Chemistry of the Reactive Nitrogen Species Peroxynitrite and Nitrogen Dioxide Generated by Physical Plasmas

Biomolecules ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1687
Author(s):  
Giuliana Bruno ◽  
Sebastian Wenske ◽  
Jan-Wilm Lackmann ◽  
Michael Lalk ◽  
Thomas von Woedtke ◽  
...  
Keyword(s):  
Reactive Nitrogen Species ◽  
High Resolution Mass Spectrometry ◽  
Clinical Effectiveness ◽  
Covalent Modification ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Plasma Parameters ◽  
Cold Plasmas ◽  
Major Application

Cold physical plasmas modulate cellular redox signaling processes, leading to the evolution of a number of clinical applications in recent years. They are a source of small reactive species, including reactive nitrogen species (RNS). Wound healing is a major application and, as its physiology involves RNS signaling, a correlation between clinical effectiveness and the activity of plasma-derived RNS seems evident. To investigate the type and reactivity of plasma-derived RNS in aqueous systems, a model with tyrosine as a tracer was utilized. By high-resolution mass spectrometry, 26 different tyrosine derivatives including the physiologic nitrotyrosine were identified. The product pattern was distinctive in terms of plasma parameters, especially gas phase composition. By scavenger experiments and isotopic labelling, gaseous nitric dioxide radicals and liquid phase peroxynitrite ions were determined as dominant RNS. The presence of water molecules in the active plasma favored the generation of peroxynitrite. A pilot study, identifying RNS driven post-translational modifications of proteins in healing human wounds after the treatment with cold plasma (kINPen), demonstrated the presence of in vitro determined chemical pathways. The plasma-driven nitration and nitrosylation of tyrosine allows the conclusion that covalent modification of biomolecules by RNS contributes to the clinically observed impact of cold plasmas.

In vitro reduction of trypsin inhibitor by purified NADPH/thioredoxin system from sprouts of sweet potato (Ipomoea batatas (L) Lam.) storage roots

Plant Science ◽  
2004 ◽  
Vol 166 (2) ◽  
pp. 435-441 ◽  
Author(s):  
Dong-Jiann Huang ◽  
Hsien-Jung Chen ◽  
Wen-chi Hou ◽  
Tzeng-Err Chen ◽  
Yaw-Huei Lin
Keyword(s):  
Sweet Potato ◽  
Trypsin Inhibitor ◽  
Ipomoea Batatas ◽  
Storage Roots ◽  
Thioredoxin System

scholarly journals Host-derived Reactive Nitrogen Species mediate the Cryptococcus neoformans yeast-to-titan switch via fungal-derived superoxide

2021 ◽  
Author(s):  
Xin Zhou ◽  
Guillaume E. Desanti ◽  
Robin C May ◽  
Ivy M Dambuza ◽  
Elizabeth R Ballou
Keyword(s):  
Cryptococcus Neoformans ◽  
Reactive Nitrogen Species ◽  
Molecular Mechanisms ◽  
Protein A ◽  
Genotoxic Stress ◽  
Strand Break ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Sod Activity

In the host lung, the human fungal pathogen Cryptococcus neoformans undergoes a morphological switch from small haploid yeast to large polyploid titan cell, contributing to C. neoformans virulence. Titan cells are less readily phagocytosed and can survive host nitrosative and oxidative stresses. We and others previously showed that titanization is triggered by host-relevant signals including CO2 and lung-resident bacteria, and addition of these factor is sufficient to induce titan cells in vitro. Here we investigate the molecular mechanisms that drive this transition and demonstrate that host-derived immune signals can increase the degree and frequency of titanization. Specifically, host-relevant reactive nitrogen species increase the accumulation of endogenous superoxide within cryptococcal cells, particularly within nuclei, where it can cause genotoxic stress. Consistent with this, we observe the accumulation of Rad51 protein, a marker of the double strand break repair pathway, in titanizing cultures. Blocking superoxide accumulation inhibits titanization, yet titanization also requires superoxide detoxification through Superoxide Dismutase (SOD) activity. Loss of mitochondrial Sod2 activity locks cells in the yeast phase, while Sod1 is required for the production of viable titan daughter cells. We hypothesize that the redox responsive transcription factor Yap1 in part mediates this response by regulating SOD2/SOD1. In addition, we show that Sod1 translocates to the nucleus, where it is likely involved in the detoxification of genotoxic superoxide. Together, these findings reveal a major new regulatory mechanism for the yeast-to-titan transition.

Reactive Nitrogen Species Modulate the Effects of Rhein, an Active Component of Senna Laxatives, on Human Epithelium In Vitro

2002 ◽  
Vol 34 (5) ◽  
pp. 529-534 ◽  
Author(s):  
Francesco Raimondi ◽  
Pasquale Santoro ◽  
Luigi Maiuri ◽  
Marco Londei ◽  
Simona Annunziata ◽  
...  
Keyword(s):  
Reactive Nitrogen Species ◽  
Active Component ◽  
Reactive Nitrogen ◽  
Nitrogen Species

Silica nanoparticles based on an AIE-active molecule for ratiometric detection of RNS in vitro

2017 ◽  
Vol 5 (46) ◽  
pp. 9197-9203 ◽  
Author(s):  
Leijing Liu ◽  
Fengli Zhang ◽  
Bin Xu ◽  
Wenjing Tian
Keyword(s):  
Silica Nanoparticles ◽  
Reactive Nitrogen Species ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Active Molecule ◽  
Ratiometric Detection

Silica nanoparticles based on an AIE-active molecule were prepared for ratiometric detection of reactive nitrogen species (RNS) in vitro.

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