Efficient red luminogen with aggregation-induced emission for in vivo three-photon brain vascular imaging

2020 ◽  
Vol 4 (6) ◽  
pp. 1634-1642 ◽  
Author(s):  
Haiyan Tian ◽  
Dongyu Li ◽  
Xi Tang ◽  
Yubo Zhang ◽  
Zhiyong Yang ◽  
...  
Keyword(s):  
High Resolution ◽  
Penetration Depth ◽  
Vascular Imaging ◽  
Quantum Yields ◽  
High Signal ◽  
Aggregation Induced Emission ◽  
Background Ratio ◽  
High Quantum

DCPE-TPA exhibits three morphologies with high quantum yields and nanoparticles of DCPE-TPA are utilized for in vivo 3PF imaging, achieving a penetration depth of 300 μm with a high resolution of 1.8 μm and a high signal-to-background ratio of 14.

scholarly journals High-speed high-resolution laser diode-based photoacoustic microscopy for in vivo microvasculature imaging

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Xiufeng Li ◽  
Victor T C Tsang ◽  
Lei Kang ◽  
Yan Zhang ◽  
Terence T W Wong
Keyword(s):  
High Resolution ◽  
High Speed ◽  
High Performance ◽  
Continuous Wave ◽  
Signal To Noise Ratio ◽  
Cost Effective ◽  
High Signal ◽  
Photoacoustic Microscopy ◽  
Mouse Ear

AbstractLaser diodes (LDs) have been considered as cost-effective and compact excitation sources to overcome the requirement of costly and bulky pulsed laser sources that are commonly used in photoacoustic microscopy (PAM). However, the spatial resolution and/or imaging speed of previously reported LD-based PAM systems have not been optimized simultaneously. In this paper, we developed a high-speed and high-resolution LD-based PAM system using a continuous wave LD, operating at a pulsed mode, with a repetition rate of 30 kHz, as an excitation source. A hybrid scanning mechanism that synchronizes a one-dimensional galvanometer mirror and a two-dimensional motorized stage is applied to achieve a fast imaging capability without signal averaging due to the high signal-to-noise ratio. By optimizing the optical system, a high lateral resolution of 4.8 μm has been achieved. In vivo microvasculature imaging of a mouse ear has been demonstrated to show the high performance of our LD-based PAM system.

scholarly journals Ultrabright red AIEgens for two-photon vascular imaging with high resolution and deep penetration

2018 ◽  
Vol 9 (10) ◽  
pp. 2705-2710 ◽  
Author(s):  
Wei Qin ◽  
Pengfei Zhang ◽  
Hui Li ◽  
Jacky W. Y. Lam ◽  
Yuanjing Cai ◽  
...  
Keyword(s):  
High Resolution ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
High Contrast ◽  
Deep Tissue ◽  
Two Photon ◽  
High Penetration ◽  
Deep Tissue Imaging

A successful strategy for the design of ultrabright red luminogens with aggregation-induced emission (AIE) features is reported. The AIE dots can be utilized as efficient fluorescent probes for in vivo deep-tissue imaging with high penetration depth and high contrast.

Biocompatible aggregation-induced emission nanoparticles with red emission for in vivo three-photon brain vascular imaging

2017 ◽  
Vol 5 (15) ◽  
pp. 2757-2762 ◽  
Author(s):  
Hequn Zhang ◽  
Nuernisha Alifu ◽  
Tao Jiang ◽  
Zhenggang Zhu ◽  
Yalun Wang ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Cross Sections ◽  
Vascular Imaging ◽  
Red Emission ◽  
Aggregation Induced Emission

AIE nanoparticles with large 3PA cross-sections were used for 3PL in vivo angiography of mouse brain, with a depth of 500 μm.

Molecular engineering of a high quantum yield NIR-II molecular fluorophore with aggregation-induced emission (AIE) characteristics for in vivo imaging

Nanoscale ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 5084-5090 ◽  
Author(s):  
Pengfei Xu ◽  
Fei Kang ◽  
Weidong Yang ◽  
Mingru Zhang ◽  
Ruili Dang ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Molecular Engineering ◽  
High Quantum Yield ◽  
Aggregation Induced Emission ◽  
High Quantum

Novel biocompatible NIR-II fluorophore with AIE characteristics has been developed and used for NIR-II biomedical fluorescence imaging.

Tantalum Oxide Nanoparticles as Versatile Contrast Agents for X-ray Computed Tomography

2020 ◽  
Author(s):  
Shatadru Chakravarty ◽  
Jeremy Hix ◽  
Kaitlyn Wieweora ◽  
Maximilian Volk ◽  
Elizabeth Kenyon ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Contrast Agents ◽  
Mesoporous Silica Nanoparticles ◽  
Sol Gel ◽  
Tantalum Oxide ◽  
High Signal ◽  
Drug Delivery Vehicles ◽  
X Ray Computed

Here we describe the synthesis, characterization and in vitro and in vivo performance of a series of tantalum oxide (TaOx) based nanoparticles (NPs) for computed tomography (CT). Five distinct versions of 9-12 nm diameter silane coated TaOx nanocrystals (NCs) were fabricated by a sol-gel method with varying degrees of hydrophilicity and with or without fluorescence, with the highest reported Ta content to date (78%). Highly hydrophilic NCs were left bare and were evaluated in vivo in mice for micro-CT of full body vasculature, where following intravenous injection, TaOx NCs demonstrate high CT contrast, circulation in blood for ~ 3 h, and eventual accumulation in RES organs; and following injection locally in the mammary gland, where the full ductal tree structure can be clearly delineated. Partially hydrophilic NCs were encapsulated within mesoporous silica nanoparticles (MSNPs; TaOx@MSNPs) and hydrophobic NCs were encapsulated within poly(lactic-co-glycolic acid) (PLGA; TaOx@PLGA) NPs, serving as potential CT-imagable drug delivery vehicles. Bolus intramuscular injections of TaOx@PLGA NPs and TaOx@MSNPs to mimic the accumulation of NPs at a tumor site produce high signal enhancement in mice. In vitro studies on bare NCs and formuated NPs demonstrate high cytocompatibility and low dissolution of TaOx. This work solidifies that TaOx-based NPs are versatile contrast agents for CT.

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