Biocompatible aggregation-induced emission nanoparticles with red emission for in vivo three-photon brain vascular imaging

2017 ◽  
Vol 5 (15) ◽  
pp. 2757-2762 ◽  
Author(s):  
Hequn Zhang ◽  
Nuernisha Alifu ◽  
Tao Jiang ◽  
Zhenggang Zhu ◽  
Yalun Wang ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Cross Sections ◽  
Vascular Imaging ◽  
Red Emission ◽  
Aggregation Induced Emission

AIE nanoparticles with large 3PA cross-sections were used for 3PL in vivo angiography of mouse brain, with a depth of 500 μm.

Efficient red luminogen with aggregation-induced emission for in vivo three-photon brain vascular imaging

2020 ◽  
Vol 4 (6) ◽  
pp. 1634-1642 ◽  
Author(s):  
Haiyan Tian ◽  
Dongyu Li ◽  
Xi Tang ◽  
Yubo Zhang ◽  
Zhiyong Yang ◽  
...  
Keyword(s):  
High Resolution ◽  
Penetration Depth ◽  
Vascular Imaging ◽  
Quantum Yields ◽  
High Signal ◽  
Aggregation Induced Emission ◽  
Background Ratio ◽  
High Quantum

DCPE-TPA exhibits three morphologies with high quantum yields and nanoparticles of DCPE-TPA are utilized for in vivo 3PF imaging, achieving a penetration depth of 300 μm with a high resolution of 1.8 μm and a high signal-to-background ratio of 14.

scholarly journals AIE-nanoparticle assisted ultra-deep microscopy in the in vivo mouse brain under 1300-nm excitation

2020 ◽  
Author(s):  
Dong-yu Li ◽  
He-qun Zhang ◽  
Lina L. Streich ◽  
Ping Lu ◽  
Ling Wang ◽  
...  
Keyword(s):  
Cross Section ◽  
Mouse Brain ◽  
Absorption Cross Section ◽  
Fluorescence Probes ◽  
Absorption Cross ◽  
Aggregation Induced Emission ◽  
Photon Excitation ◽  
Photon Imaging

AbstractAggregation-induced emission nanoparticles serve as promising fluorescence probes for multi-photon excitation microscopy due to the large absorption cross-section at NIR-IIb region. Here we present organic AIE nanoparticles that feature high aborption cross-section under three-photon excitation. We show that these enable ultra-deep NIR-IIa excited three-photon imaging in the in-vivo mouse brain.

scholarly journals AIE-nanoparticle assisted ultra-deep three-photon microscopy in the in vivo mouse brain under 1300-nm excitation

2021 ◽  
Author(s):  
Dongyu Li ◽  
Hequn Zhang ◽  
Lina Streich ◽  
Yalun Wang ◽  
Ping Lu ◽  
...  
Keyword(s):  
Cross Section ◽  
Mouse Brain ◽  
Absorption Cross Section ◽  
Fluorescence Probes ◽  
Absorption Cross ◽  
Aggregation Induced Emission ◽  
Photon Excitation

Aggregation-induced emission nanoparticles serve as promising fluorescence probes for multi-photon excitation microscopy due to the large absorption cross-section at NIR-IIb region. Here we present organic AIE nanoparticles that feature high...

Differential up-regulation of hypoxia-inducible vasoactive factors in fetal mouse brain in vivo upon intrauterine hypoxia

2006 ◽  
Vol 37 (03) ◽  
Author(s):  
R Trollmann ◽  
K Strasser ◽  
J Soliz ◽  
D Wenzel ◽  
W Rascher ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Fetal Mouse ◽  
Vasoactive Factors ◽  
Intrauterine Hypoxia

Sensitive and specific detection of peroxynitrite and in-vivo imaging of inflammation by a "simple" AIE bioprobe

2021 ◽  
Author(s):  
Huilin Xie ◽  
Jingtian Zhang ◽  
Chao Chen ◽  
Feiyi Sun ◽  
Haixiang Liu ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescence Enhancement ◽  
Specific Detection ◽  
Aggregation Induced Emission

A luminogenic bioprobe TPE-DMAB for simple and specific detection of peroxynitrite (ONOO−) has been developed. TPE-DMAB exhibits aggregation-induced emission (AIE) characteristic and shows fluorescence enhancement (up to 100-fold) upon cleavage...

scholarly journals 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jun Zhu ◽  
Hercules Rezende Freitas ◽  
Izumi Maezawa ◽  
Lee-way Jin ◽  
Vivek J. Srinivasan
Keyword(s):  
Minimally Invasive ◽  
Mouse Brain ◽  
Numerical Aperture ◽  
Optical Biopsy ◽  
Optical Coherence ◽  
Label Free ◽  
Optical Coherence Microscopy ◽  
Minimal Invasiveness ◽  
Cortical Regions

AbstractIn vivo, minimally invasive microscopy in deep cortical and sub-cortical regions of the mouse brain has been challenging. To address this challenge, we present an in vivo high numerical aperture optical coherence microscopy (OCM) approach that fully utilizes the water absorption window around 1700 nm, where ballistic attenuation in the brain is minimized. Key issues, including detector noise, excess light source noise, chromatic dispersion, and the resolution-speckle tradeoff, are analyzed and optimized. Imaging through a thinned-skull preparation that preserves intracranial space, we present volumetric imaging of cytoarchitecture and myeloarchitecture across the entire depth of the mouse neocortex, and some sub-cortical regions. In an Alzheimer’s disease model, we report that findings in superficial and deep cortical layers diverge, highlighting the importance of deep optical biopsy. Compared to other microscopic techniques, our 1700 nm OCM approach achieves a unique combination of intrinsic contrast, minimal invasiveness, and high resolution for deep brain imaging.

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