scholarly journals Bio-orthogonal triazolinedione (TAD) crosslinked protein nanocapsules affect protein adsorption and cell interaction

2020 ◽  
Vol 11 (23) ◽  
pp. 3821-3830 ◽  
Author(s):  
Marie-Luise Frey ◽  
Johanna Simon ◽  
Maximilian Brückner ◽  
Volker Mailänder ◽  
Svenja Morsbach ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Click Chemistry ◽  
Protein Adsorption ◽  
Drug Delivery Systems ◽  
Cell Interaction ◽  
Delivery Systems ◽  
High Loading ◽  
Loading Capacity ◽  
Low Toxicity

Albumin-based protein nanocarriers obtained by TAD click chemistry have been widely exploited as drug delivery systems, since they show excellent degradability, low toxicity, but at the same time provide high loading capacity and relevant uptake into cells.

scholarly journals Natural Polysaccharides for siRNA Delivery: Nanocarriers Based on Chitosan, Hyaluronic Acid, and Their Derivatives

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2570 ◽  
Author(s):  
Inés Serrano-Sevilla ◽  
Álvaro Artiga ◽  
Scott G. Mitchell ◽  
Laura De Matteis ◽  
Jesús M. de la Fuente
Keyword(s):  
Drug Delivery ◽  
Hyaluronic Acid ◽  
Small Interfering Rna ◽  
Drug Delivery Systems ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Low Toxicity ◽  
Interfering Rna

Natural polysaccharides are frequently used in the design of drug delivery systems due to their biocompatibility, biodegradability, and low toxicity. Moreover, they are diverse in structure, size, and charge, and their chemical functional groups can be easily modified to match the needs of the final application and mode of administration. This review focuses on polysaccharidic nanocarriers based on chitosan and hyaluronic acid for small interfering RNA (siRNA) delivery, which are highly positively and negatively charged, respectively. The key properties, strengths, and drawbacks of each polysaccharide are discussed. In addition, their use as efficient nanodelivery systems for gene silencing applications is put into context using the most recent examples from the literature. The latest advances in this field illustrate effectively how chitosan and hyaluronic acid can be modified or associated with other molecules in order to overcome their limitations to produce optimized siRNA delivery systems with promising in vitro and in vivo results.

scholarly journals Study of subchronic toxicity of the developed antibacterial drug based on exosomal particles

2020 ◽  
Vol 175 ◽  
pp. 07002
Author(s):  
Elena Kastarnova ◽  
Vladimir Orobets ◽  
Valeria Shahova ◽  
Olga Sevostyanova ◽  
Ilya Belyaev ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Antibacterial Drug ◽  
Subchronic Toxicity ◽  
Respiratory Epithelial Cells ◽  
White Rats ◽  
Safety Parameters ◽  
Low Toxicity ◽  
Respiratory Epithelial

One of the main promising directions in the development of pharmacology is the development of drugs that provide targeted drug concentration and effective approaches to obtaining them using nanoscale drug delivery systems. In our opinion, the most promising is the use of biodegradable delivery systems due to their low toxicity and xenobiotic effects on the patient’s body. The need to develop regulated drug delivery systems is due to their clear advantage over analogues in standardized dosage forms. Based on modern research publications we have developed an exosomal antibacterial form of azithromycin that has pronounced selective properties for respiratory epithelial cells. The aim of the work was to study the safety parameters of the developed drug based on exosomal particles, in particular subchronic toxicity. For the study 3 experimental and 1 control groups of white rats weighing 190-220 g were formed with 10 heads each. The drug was administered intragastrically daily for 90 days in doses of 1/10; 1/20 and 1/50 of the LD50 set in the acute experiment. The study found that the use of the developed exosomal drug intragastrically once a day for 90 days does not affect the behavioral responses of animals and their external condition.

scholarly journals Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

2017 ◽  
Vol 8 ◽  
pp. 1457-1468 ◽  
Author(s):  
Gamze Varan ◽  
Juan M Benito ◽  
Carmen Ortiz Mellet ◽  
Erem Bilensoy
Keyword(s):  
Drug Delivery ◽  
Cell Line ◽  
Anticancer Drug ◽  
Drug Delivery Systems ◽  
Drug Carrier ◽  
Delivery Systems ◽  
Loading Capacity ◽  
Promising Alternative ◽  
Cytotoxicity Studies ◽  
Amphiphilic Cyclodextrin

Background: Paclitaxel is a potent anticancer drug that is effective against a wide spectrum of cancers. To overcome its bioavailability problems arising from very poor aqueous solubility and tendency to recrystallize upon dilution, paclitaxel is commercially formulated with co-solvents such as Cremophor EL® that are known to cause serious side effects during chemotherapy. Amphiphilic cyclodextrins are favored oligosaccharides as drug delivery systems for anticancer drugs, having the ability to spontaneously form nanoparticles without surfactant or co-solvents. In the past few years, polycationic, amphiphilic cyclodextrins were introduced as effective agents for gene delivery in the form of nanoplexes. In this study, the potential of polycationic, amphiphilic cyclodextrin nanoparticles were evaluated in comparison to non-ionic amphiphilic cyclodextrins and core–shell type cyclodextrin nanoparticles for paclitaxel delivery to breast tumors. Pre-formulation studies were used as a basis for selecting the suitable organic solvent and surfactant concentration for the novel polycationic cyclodextrin nanoparticles. The nanoparticles were then extensively characterized with particle size distribution, polydispersity index, zeta potential, drug loading capacity, in vitro release profiles and cytotoxicity studies. Results: Paclitaxel-loaded cyclodextrin nanoparticles were obtained in the diameter range of 80−125 nm (depending on the nature of the cyclodextrin derivative) where the smallest diameter nanoparticles were obtained with polycationic (PC) βCDC6. A strong positive charge also helped to increase the loading capacity of the nanoparticles with paclitaxel up to 60%. Interestingly, cyclodextrin nanoparticles were able to stabilize paclitaxel in aqueous solution for 30 days. All blank cyclodextrin nanoparticles were demonstrated to be non-cytotoxic against L929 mouse fibroblast cell line. In addition, paclitaxel-loaded nanoparticles have a significant anticancer effect against MCF-7 human breast cancer cell line as compared with a paclitaxel solution in DMSO. Conclusion: According to the results of this study, both amphiphilic cyclodextrin derivatives provide suitable nanometer-sized drug delivery systems for safe and efficient intravenous paclitaxel delivery for chemotherapy. In the light of these studies, it can be said that amphiphilic cyclodextrin nanoparticles of different surface charge can be considered as a promising alternative for self-assembled nanometer-sized drug carrier systems for safe and efficient chemotherapy.

scholarly journals Aptamers Chemistry: Chemical Modifications and Conjugation Strategies

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 3 ◽  
Author(s):  
Fadwa Odeh ◽  
Hamdi Nsairat ◽  
Walhan Alshaer ◽  
Mohammad A. Ismail ◽  
Ezaldeen Esawi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Pharmacological Properties ◽  
Chemical Modifications ◽  
New Class ◽  
Advantages And Disadvantages ◽  
Dna Oligonucleotides ◽  
Low Toxicity ◽  
Group Diversity

Soon after they were first described in 1990, aptamers were largely recognized as a new class of biological ligands that can rival antibodies in various analytical, diagnostic, and therapeutic applications. Aptamers are short single-stranded RNA or DNA oligonucleotides capable of folding into complex 3D structures, enabling them to bind to a large variety of targets ranging from small ions to an entire organism. Their high binding specificity and affinity make them comparable to antibodies, but they are superior regarding a longer shelf life, simple production and chemical modification, in addition to low toxicity and immunogenicity. In the past three decades, aptamers have been used in a plethora of therapeutics and drug delivery systems that involve innovative delivery mechanisms and carrying various types of drug cargos. However, the successful translation of aptamer research from bench to bedside has been challenged by several limitations that slow down the realization of promising aptamer applications as therapeutics at the clinical level. The main limitations include the susceptibility to degradation by nucleases, fast renal clearance, low thermal stability, and the limited functional group diversity. The solution to overcome such limitations lies in the chemistry of aptamers. The current review will focus on the recent arts of aptamer chemistry that have been evolved to refine the pharmacological properties of aptamers. Moreover, this review will analyze the advantages and disadvantages of such chemical modifications and how they impact the pharmacological properties of aptamers. Finally, this review will summarize the conjugation strategies of aptamers to nanocarriers for developing targeted drug delivery systems.

scholarly journals Novel Delivery Systems of Polyphenols and Their Potential Health Benefits

2021 ◽  
Vol 14 (10) ◽  
pp. 946
Author(s):  
Bianca Enaru ◽  
Sonia Socaci ◽  
Anca Farcas ◽  
Carmen Socaciu ◽  
Corina Danciu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Environmental Factors ◽  
Active Compound ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Potential Health ◽  
Future Studies ◽  
Beneficial Effects ◽  
Low Toxicity ◽  
Novel Delivery Systems

Liposome-based delivery systems have been studied and used more frequently in recent years due to their advantages, such as low toxicity, specificity, and the ability to protect the encapsulated substance from environmental factors, which could otherwise degrade the active compound and reduce its effectiveness. Given these benefits, many researchers have encapsulated polyphenols in liposomes, thus increasing their bioavailability and stability. Similarly, polyphenols encapsulated in liposomes are known to produce more substantial effects on targeted cells than unencapsulated polyphenols, while having minimal cytotoxicity in healthy cells. Although polyphenols play a role in preventing many types of disease and generally have beneficial effects on health, we solely focused on their chemopreventive effects on cancer through liposomes in this review. Our goal was to summarize the applicability and efficacy of liposomes encapsulated with different classes of polyphenols on several types of cancer, thus opening the opportunity for future studies based on these drug delivery systems.

The potential application of novel drug delivery systems for phytopharmaceuticals and natural extracts – current status and future perspectives

2020 ◽  
Vol 20 ◽  
Author(s):  
Mohamad Fawzi Mahomoodally ◽  
Nabeelah Sadeer ◽  
Mehtaab Edoo ◽  
Katharigatta N. Venugopala
Keyword(s):  
Drug Delivery ◽  
Therapeutic Efficacy ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Current Status ◽  
Novel Technologies ◽  
Alternative Drug ◽  
Low Toxicity ◽  
Novel Drug ◽  
Herbal Formulations

: Phytocompounds isolated from plants are well appraised for their broad pharmacological propensities in several pathologies. One key benefit of phytoconstituents is their relatively low toxicity and adverse effects. Nonetheless, poor solubility, permeation, and poor specificity at the target site tend to hinder its therapeutic efficacy. Hence, novel technologies for drug delivery systems are being developed via the use of various nanoformulation strategies to overcome these challenges and give uniform medication focusing at the dynamic site in desired concentration and improved therapeutic efficacy. Such approaches comprise of novel drug delivery systems (NDDS). The utilisation of herbal formulations for NDDS is more beneficious and advantageous as opposed to others. The utilisation of ethosome, liposome, emulsion, phytosomes, microsphere, and strong lipid nanoparticles of herbal formulation have improved the remedial impacts of plant extricates. With the utilisation of all these, directed delivery of the formulation is accomplished, because of which the formulation exhibits impact on the site, and the bioavailability of the formulation is likewise expanded. With these novel medication conveyance frameworks, the actives and concentrates which are utilised as part of natural formulations exhibit sustained release of formulation, enhancement in stability, improved therapeutic efficacy, and protection from toxicity. The primary motivation behind creating alternative drug delivery technologies is to expand the effectiveness of drug conveyance and safety in the process of drug conveyance and give more comfort to the patient. In this review, the importance of various phytocompounds in the delivery of drugs are highlighted as well as their importance in reducing the risk or diseases.

“Click” functionalization of dual stimuli-responsive polymer nanocapsules for drug delivery systems

2017 ◽  
Vol 8 (20) ◽  
pp. 3056-3065 ◽  
Author(s):  
Wen Jing Yang ◽  
Tingting Zhao ◽  
Peng Zhou ◽  
Simou Chen ◽  
Yu Gao ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Click Chemistry ◽  
Surface Functionalization ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Stimuli Responsive ◽  
Responsive Polymer ◽  
Stimuli Responsive Polymer

“Clickable” and dual stimuli-responsive nanocapsules were developed for facile surface functionalizationviathiol–yne click chemistry and employed as drug nano-carriers.

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