Structure-guided design, generation, and biofunction of PEGylated fibroblast growth factor 2 variants for wound healing

Nanoscale ◽  
2020 ◽  
Vol 12 (35) ◽  
pp. 18200-18213
Author(s):  
Jian Sun ◽  
Jiamin Wu ◽  
Hui Jin ◽  
Te Ying ◽  
Wei Jin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Skin Repair ◽  
Improved Stability ◽  
Design Generation

Structure-guided PEGylation of fibroblast growth factor 2 with improved stability and bioactivity for skin repair.

Stimulation of wound healing by PU/hydrogel composites containing fibroblast growth factor-2

2015 ◽  
Vol 3 (9) ◽  
pp. 1931-1941 ◽  
Author(s):  
Yiu-Jiuan Lin ◽  
Ga-Hwa Lee ◽  
Chih-Wei Chou ◽  
Yi-Peng Chen ◽  
Te-Hsing Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Histological Examination ◽  
Wound Repair ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Hydrogel Composites ◽  
Stimulation Of Wound Healing ◽  
Stimulation Of

Histological examination of wound repair in each group on day 2 after initial wounding. The arrows indicate the vascularization formation.

Photocrosslinkable chitosan hydrogel containing fibroblast growth factor-2 stimulates wound healing in healing-impaired db/db mice

Biomaterials ◽  
2003 ◽  
Vol 24 (20) ◽  
pp. 3437-3444 ◽  
Author(s):  
Kiyohaya Obara ◽  
Masayuki Ishihara ◽  
Toshiaki Ishizuka ◽  
Masanori Fujita ◽  
Yuichi Ozeki ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Chitosan Hydrogel

scholarly journals Collagen Hydrogel Scaffold and Fibroblast Growth Factor-2 Accelerate Periodontal Healing of Class II Furcation Defects in Dog

2016 ◽  
Vol 10 (1) ◽  
pp. 347-359 ◽  
Author(s):  
Takehito Momose ◽  
Hirofumi Miyaji ◽  
Akihito Kato ◽  
Kosuke Ogawa ◽  
Takashi Yoshida ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Class Ii ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Hydrogel Scaffold ◽  
Collagen Hydrogel ◽  
Furcation Defects ◽  
Periodontal Wound Healing

Objective: Collagen hydrogel scaffold exhibits bio-safe properties and facilitates periodontal wound healing. However, regenerated tissue volume is insufficient. Fibroblast growth factor-2 (FGF2) up-regulates cell behaviors and subsequent wound healing. We evaluated whether periodontal wound healing is promoted by application of collagen hydrogel scaffold in combination with FGF2 in furcation defects in beagle dogs. Methods: Collagen hydrogel was fabricated from bovine type I collagen with an ascorbate-copper ion cross-linking system. Collagen hydrogel was mingled with FGF2 and injected into sponge-form collagen. Subsequently, FGF2 (50 µg)/collagen hydrogel scaffold and collagen hydrogel scaffold alone were implanted into class II furcation defects in dogs. In addition, no implantation was performed as a control. Histometric parameters were assessed at 10 days and 4 weeks after surgery. Result: FGF2 application to scaffold promoted considerable cell and tissue ingrowth containing numerous cells and blood vessel-like structure at day 10. At 4 weeks, reconstruction of alveolar bone was stimulated by implantation of scaffold loaded with FGF2. Furthermore, periodontal attachment, consisting of cementum-like tissue, periodontal ligament-like tissue and Sharpey’s fibers, was also repaired, indicating that FGF2-loaded scaffold guided self-assembly and then re-established the function of periodontal organs. Aberrant healing, such as ankylosis and root resorption, was not observed. Conclusion: FGF2-loaded collagen hydrogel scaffold possessed excellent biocompatibility and strongly promoted periodontal tissue engineering, including periodontal attachment re-organization.

Acceleration of wound healing in healing-impaired db/db mice with a photocrosslinkable chitosan hydrogel containing fibroblast growth factor-2

2005 ◽  
Vol 13 (4) ◽  
pp. 390-397 ◽  
Author(s):  
Kiyohaya Obara ◽  
Masayuki Ishihara ◽  
Masanori Fujita ◽  
Yasuhiro Kanatani ◽  
Hidemi Hattori ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Chitosan Hydrogel

scholarly journals Fibroblast Growth Factor 2 Augments Transforming Growth Factor Beta 1 Induced Epithelial-mesenchymal Transition in Lung Cell Culture Model

2020 ◽  
Author(s):  
Lamis M.F. El-Baz ◽  
Nahla M. Shoukry ◽  
Hani S. Hafez ◽  
Robert D. Guzy ◽  
Mohamed Labib Salem
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Epithelial Mesenchymal Transition ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Mesenchymal Transition ◽  
Lung Epithelial

Impaired lung epithelial cell regeneration following injury may contribute to the development of pulmonary fibrosis. Epithelial-mesenchymal transition (EMT) is a critical event in embryonic development, wound healing following injury, and even cancer progression. Previous studies have shown that the combination of transforming growth factor beta-1 (TGFβ1) and fibroblast growth factor 2 (FGF2) induces EMT during cancer metastasis. However, this synergy remains to be elucidated in inducing EMT associated with wound healing after injury. We set out this study to determine the effect of fibroblast growth factor 2 (FGF2) on TGFβ1-induced EMT in the human lung epithelium. BEAS-2B and A549 cells were treated with TGFβ1, FGF2, or both. EMT phenotype was investigated morphologically and by measuring mRNA expression levels; using quantitative real-time PCR. E-cadherin expression was assayed by western blot and immunofluorescence staining. Cell migration was confirmed using a wound-healing assay. TGFβ1 induced a morphological change and a significant increase in cell migration of BEAS-2B cells. TGFβ1 significantly reduced E-cadherin (CDH1) mRNA expression and markedly induced expression of N-cadherin (CDH2), tenascin C (TNC), fibronectin (FN), actin alpha 2 (ACTA2), and collagen I (COL1A1). While FGF2 alone did not significantly alter EMT gene expression, it enhanced TGFβ1-induced suppression of CDH1 and upregulation of ACTA2, but not TNC, FN, and CDH2. FGF2 significantly inhibited TGFβ1-induced COL1A1 expression. Furthermore, FGF2 maintained TGFβ1-induced morphologic changes and increased the migration of TGFβ1-treated cells. This study suggests a synergistic effect between TGFβ1 and FGF2 in inducing EMT in lung epithelial cells, which may play an important role in wound healing and tissue repair after injury.

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