scholarly journals Amphiphilic gold nanoparticles perturb phase separation in multidomain lipid membranes

Nanoscale ◽  
2020 ◽  
Vol 12 (38) ◽  
pp. 19746-19759
Author(s):  
Ester Canepa ◽  
Sebastian Salassi ◽  
Anna Lucia de Marco ◽  
Chiara Lambruschini ◽  
Davide Odino ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Phase Separation ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Amphiphilic Nanoparticles

Experiments and simulations reveal that amphiphilic nanoparticles suppress phase separation in neuronal-like lipid bilayers and form bilayer-embedded ordered aggregates.

scholarly journals Charge-induced phase separation in lipid membranes

Soft Matter ◽  
2014 ◽  
Vol 10 (40) ◽  
pp. 7959-7967 ◽  
Author(s):  
Hiroki Himeno ◽  
Naofumi Shimokawa ◽  
Shigeyuki Komura ◽  
David Andelman ◽  
Tsutomu Hamada ◽  
...  
Keyword(s):  
Phase Separation ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Membrane Phase ◽  
Lipid Mixtures ◽  
The Stability

Phase separation in lipid bilayers is examined. We observed phase-separated structures in various lipid mixtures and determined membrane miscibility temperatures. It was found that a combination of negatively-charged heads and saturation of hydrocarbon tails is dominant for the stability of membrane phase separation.

scholarly journals Imaging phase separation in model lipid membranes through the use of BODIPY based molecular rotors

2015 ◽  
Vol 17 (28) ◽  
pp. 18393-18402 ◽  
Author(s):  
Michael R. Dent ◽  
Ismael López-Duarte ◽  
Callum J. Dickson ◽  
Niall D. Geoghegan ◽  
Jonathan M. Cooper ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Phase Separation ◽  
Fluorescence Spectroscopy ◽  
Molecular Dynamics Simulations ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Molecular Rotors ◽  
Model Lipid Membranes ◽  
Dynamics Simulations

Viscosity in the phase-separated lipid bilayers is investigated through the use of fluorescence spectroscopy and molecular dynamics simulations.

Observation of Concanavalin-A receptors on hydrated planar erythrocyte membrane film by in situ electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 608-609
Author(s):  
Neng-Bo He ◽  
S.W. Hui
Keyword(s):  
Lipid Bilayers ◽  
Erythrocyte Membrane ◽  
Lipid Membranes ◽  
Surface Film ◽  
Biological Membranes ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Black Lipid Membranes ◽  
Fine Mesh ◽  
Planar Films

Monolayers and planar "black" lipid membranes have been widely used as models for studying the structure and properties of biological membranes. Because of the lack of a suitable method to prepare these membranes for electron microscopic observation, their ultrastructure is so far not well understood. A method of forming molecular bilayers over the holes of fine mesh grids was developed by Hui et al. to study hydrated and unsupported lipid bilayers by electron diffraction, and to image phase separated domains by diffraction contrast. We now adapted the method of Pattus et al. of spreading biological membranes vesicles on the air-water interfaces to reconstitute biological membranes into unsupported planar films for electron microscopic study. hemoglobin-free human erythrocyte membrane stroma was prepared by hemolysis. The membranes were spreaded at 20°C on balanced salt solution in a Langmuir trough until a surface pressure of 20 dyne/cm was reached. The surface film was repeatedly washed by passing to adjacent troughs over shallow partitions (fig. 1).

scholarly journals Cardiolipin-Containing Lipid Membranes Attract the Bacterial Cell Division Protein DivIVA

2021 ◽  
Vol 22 (15) ◽  
pp. 8350
Author(s):  
Naďa Labajová ◽  
Natalia Baranova ◽  
Miroslav Jurásek ◽  
Robert Vácha ◽  
Martin Loose ◽  
...  
Keyword(s):  
Cell Division ◽  
Lipid Bilayers ◽  
Bacterial Cell ◽  
Lipid Membranes ◽  
Model Organism ◽  
Active Role ◽  
Membrane Curvature ◽  
Bacterial Cell Division ◽  
Division Site ◽  
Clostridioides Difficile

DivIVA is a protein initially identified as a spatial regulator of cell division in the model organism Bacillus subtilis, but its homologues are present in many other Gram-positive bacteria, including Clostridia species. Besides its role as topological regulator of the Min system during bacterial cell division, DivIVA is involved in chromosome segregation during sporulation, genetic competence, and cell wall synthesis. DivIVA localizes to regions of high membrane curvature, such as the cell poles and cell division site, where it recruits distinct binding partners. Previously, it was suggested that negative curvature sensing is the main mechanism by which DivIVA binds to these specific regions. Here, we show that Clostridioides difficile DivIVA binds preferably to membranes containing negatively charged phospholipids, especially cardiolipin. Strikingly, we observed that upon binding, DivIVA modifies the lipid distribution and induces changes to lipid bilayers containing cardiolipin. Our observations indicate that DivIVA might play a more complex and so far unknown active role during the formation of the cell division septal membrane.

scholarly journals Salt-Induced Liquid–Liquid Phase Separation and Interfacial Crystal Formation in Poly(N-isopropylacrylamide)-Capped Gold Nanoparticles

2021 ◽  
Vol 125 (9) ◽  
pp. 5349-5362
Author(s):  
Alejandra Londoño-Calderon ◽  
Wenjie Wang ◽  
Jack J. Lawrence ◽  
Wei Bu ◽  
David Vaknin ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Phase Separation ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
Crystal Formation ◽  
Liquid Liquid Phase Separation

scholarly journals The Use of Tethered Bilayer Lipid Membranes to Identify the Mechanisms of Antimicrobial Peptide Interactions with Lipid Bilayers

Antibiotics ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 12 ◽  
Author(s):  
Amani Alghalayini ◽  
Alvaro Garcia ◽  
Thomas Berry ◽  
Charles Cranfield
Keyword(s):  
New Zealand ◽  
Patch Clamp ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Antimicrobial Agents ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Black Lipid Membranes ◽  
Peptide Interactions ◽  
New Research

This review identifies the ways in which tethered bilayer lipid membranes (tBLMs) can be used for the identification of the actions of antimicrobials against lipid bilayers. Much of the new research in this area has originated, or included researchers from, the southern hemisphere, Australia and New Zealand in particular. More and more, tBLMs are replacing liposome release assays, black lipid membranes and patch-clamp electrophysiological techniques because they use fewer reagents, are able to obtain results far more quickly and can provide a uniformity of responses with fewer artefacts. In this work, we describe how tBLM technology can and has been used to identify the actions of numerous antimicrobial agents.

scholarly journals Dynamics of Phase Separation in Lipid Membranes

2010 ◽  
Vol 98 (3) ◽  
pp. 664a
Author(s):  
Brian Camley ◽  
Frank L.H. Brown
Keyword(s):  
Phase Separation ◽  
Lipid Membranes

Aromaticity/Bulkiness of Surface Ligands to Promote the Interaction of Anionic Amphiphilic Gold Nanoparticles with Lipid Bilayers

Langmuir ◽  
2016 ◽  
Vol 32 (6) ◽  
pp. 1601-1610 ◽  
Author(s):  
Jinhong Gao ◽  
Ouyang Zhang ◽  
Jing Ren ◽  
Chuanliu Wu ◽  
Yibing Zhao
Keyword(s):  
Gold Nanoparticles ◽  
Lipid Bilayers ◽  
Surface Ligands

scholarly journals Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers

Nano Letters ◽  
2013 ◽  
Vol 13 (9) ◽  
pp. 4060-4067 ◽  
Author(s):  
Reid C. Van Lehn ◽  
Prabhani U. Atukorale ◽  
Randy P. Carney ◽  
Yu-Sang Yang ◽  
Francesco Stellacci ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Lipid Bilayers ◽  
Surface Composition ◽  
Particle Diameter
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