Dynamic behavior of an artificial protein needle contacting a membrane observed by high-speed atomic force microscopy

Nanoscale ◽  
2020 ◽  
Vol 12 (15) ◽  
pp. 8166-8173 ◽  
Author(s):  
Takafumi Ueno ◽  
Kento Niwase ◽  
Daisho Tsubokawa ◽  
Kosuke Kikuchi ◽  
Natsumi Takai ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dynamic Behavior ◽  
High Speed ◽  
Molecular Level ◽  
Bacteriophage T4 ◽  
Force Microscopy ◽  
Atomic Force ◽  
Artificial Protein

An artificial protein needle designed from bacteriophage T4 can contact a membrane. The dynamic behavior is directly observed with a single-molecular level by high-speed atomic force microscopy (HS-AFM).

High-Speed Atomic Force Microscopy for Studying the Dynamic Behavior of Protein Molecules at Work

2006 ◽  
Vol 45 (3B) ◽  
pp. 1897-1903 ◽  
Author(s):  
Toshio Ando ◽  
Takayuki Uchihashi ◽  
Noriyuki Kodera ◽  
Atsushi Miyagi ◽  
Ryo Nakakita ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dynamic Behavior ◽  
High Speed ◽  
Force Microscopy ◽  
Atomic Force ◽  
Protein Molecules

scholarly journals High-speed Atomic Force Microscopy for Capturing Dynamic Behavior of Protein Molecules at Work

2005 ◽  
Vol 3 ◽  
pp. 384-392 ◽  
Author(s):  
Toshio Ando ◽  
Noriyuki Kodera ◽  
Takayuki Uchihashi ◽  
Atsushi Miyagi ◽  
Ryo Nakakita ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dynamic Behavior ◽  
High Speed ◽  
Force Microscopy ◽  
Atomic Force ◽  
Protein Molecules

scholarly journals 1SB03 Dynamic behavior of dynein and myosin V revealed by high-speed atomic force microscopy

2004 ◽  
Vol 44 (supplement) ◽  
pp. S4
Author(s):  
N. Kodera ◽  
A. Miyagi ◽  
D. Maeda ◽  
H. Sakakibara ◽  
K. Oiwa ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dynamic Behavior ◽  
High Speed ◽  
Myosin V ◽  
Force Microscopy ◽  
Atomic Force

Probing Structural Dynamics of an Artificial Protein Cage Using High-Speed Atomic Force Microscopy

Nano Letters ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 1331-1335 ◽  
Author(s):  
Motonori Imamura ◽  
Takayuki Uchihashi ◽  
Toshio Ando ◽  
Annika Leifert ◽  
Ulrich Simon ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Structural Dynamics ◽  
High Speed ◽  
Force Microscopy ◽  
Protein Cage ◽  
Atomic Force ◽  
Artificial Protein

scholarly journals High-speed atomic force microscopy highlights new molecular mechanism of daptomycin action

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Francesca Zuttion ◽  
Adai Colom ◽  
Stefan Matile ◽  
Denes Farago ◽  
Frédérique Pompeo ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Speed ◽  
Molecular Mechanisms ◽  
Molecular Level ◽  
Force Microscopy ◽  
Multi Scale ◽  
Atomic Force ◽  
Level Information ◽  
Bacterial Lipid ◽  
Membrane Deformations

AbstractThe increase in speed of the high-speed atomic force microscopy (HS-AFM) compared to that of the conventional AFM made possible the first-ever visualisation at the molecular-level of the activity of an antimicrobial peptide on a membrane. We investigated the medically prescribed but poorly understood lipopeptide Daptomycin under infection-like conditions (37 °C, bacterial lipid composition and antibiotic concentrations). We confirmed so far hypothetical models: Dap oligomerization and the existence of half pores. Moreover, we detected unknown molecular mechanisms: new mechanisms to form toroidal pores or to resist Dap action, and to unprecedently quantify the energy profile of interacting oligomers. Finally, the biological and medical relevance of the findings was ensured by a multi-scale multi-nativeness—from the molecule to the cell—correlation of molecular-level information from living bacteria (Bacillus subtilis strains) to liquid-suspended vesicles and supported-membranes using electron and optical microscopies and the lipid tension probe FliptR, where we found that the cells with a healthier state of their cell wall show smaller membrane deformations.

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