Probing Structural Dynamics of an Artificial Protein Cage Using High-Speed Atomic Force Microscopy

Nano Letters ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 1331-1335 ◽  
Author(s):  
Motonori Imamura ◽  
Takayuki Uchihashi ◽  
Toshio Ando ◽  
Annika Leifert ◽  
Ulrich Simon ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Structural Dynamics ◽  
High Speed ◽  
Force Microscopy ◽  
Protein Cage ◽  
Atomic Force ◽  
Artificial Protein

scholarly journals High-speed atomic force microscopy reveals structural dynamics of α-synuclein monomers and dimers

2018 ◽  
Vol 148 (12) ◽  
pp. 123322 ◽  
Author(s):  
Yuliang Zhang ◽  
Mohtadin Hashemi ◽  
Zhengjian Lv ◽  
Benfeard Williams ◽  
Konstantin I. Popov ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Structural Dynamics ◽  
High Speed ◽  
Force Microscopy ◽  
Atomic Force

[P1-232]: HIGH-SPEED ATOMIC FORCE MICROSCOPY REVEALS STRUCTURAL DYNAMICS OF AMYLOID β1-42 AGGREGATES

2017 ◽  
Vol 13 (7S_Part_6) ◽  
pp. P332-P333
Author(s):  
Kenjiro Ono ◽  
Takahiro Nakayama ◽  
Masahiro Itami ◽  
Ryoichi Takahashi ◽  
David B. Teplow ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Structural Dynamics ◽  
High Speed ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals High-Speed Atomic Force Microscopy Reveals the Structural Dynamics of the Amyloid-β and Amylin Aggregation Pathways

2020 ◽  
Vol 21 (12) ◽  
pp. 4287
Author(s):  
Takahiro Watanabe-Nakayama ◽  
Bikash R. Sahoo ◽  
Ayyalusamy Ramamoorthy ◽  
Kenjiro Ono
Keyword(s):  
Atomic Force Microscopy ◽  
Protein Structure ◽  
Real Time ◽  
Structural Dynamics ◽  
High Speed ◽  
Amyloid Β ◽  
Force Microscopy ◽  
Structure And Dynamics ◽  
Atomic Force ◽  
Novel Therapeutic Strategies

Individual Alzheimer’s disease (AD) patients have been shown to have structurally distinct amyloid-β (Aβ) aggregates, including fibrils, in their brain. These findings suggest the possibility of a relationship between AD progression and Aβ fibril structures. Thus, the characterization of the structural dynamics of Aβ could aid the development of novel therapeutic strategies and diagnosis. Protein structure and dynamics have typically been studied separately. Most of the commonly used biophysical approaches are limited in providing substantial details regarding the combination of both structure and dynamics. On the other hand, high-speed atomic force microscopy (HS-AFM), which simultaneously visualizes an individual protein structure and its dynamics in liquid in real time, can uniquely link the structure and the kinetic details, and it can also unveil novel insights. Although amyloidogenic proteins generate heterogeneously aggregated species, including transient unstable states during the aggregation process, HS-AFM elucidated the structural dynamics of individual aggregates in real time in liquid without purification and isolation. Here, we review and discuss the HS-AFM imaging of amyloid aggregation and strategies to optimize the experiments showing findings from Aβ and amylin, which is associated with type II diabetes, shares some common biological features with Aβ, and is reported to be involved in AD.

Dynamic behavior of an artificial protein needle contacting a membrane observed by high-speed atomic force microscopy

Nanoscale ◽  
2020 ◽  
Vol 12 (15) ◽  
pp. 8166-8173 ◽  
Author(s):  
Takafumi Ueno ◽  
Kento Niwase ◽  
Daisho Tsubokawa ◽  
Kosuke Kikuchi ◽  
Natsumi Takai ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dynamic Behavior ◽  
High Speed ◽  
Molecular Level ◽  
Bacteriophage T4 ◽  
Force Microscopy ◽  
Atomic Force ◽  
Artificial Protein

An artificial protein needle designed from bacteriophage T4 can contact a membrane. The dynamic behavior is directly observed with a single-molecular level by high-speed atomic force microscopy (HS-AFM).

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